First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.

Natural background radiation is a permanent multicomponent factor. It has an influence on biological organisms, but effects of its deprivation still remain unclear. The aim of our work was to study for the first time responses of D. melanogaster to conditions of the Deep Underground Low-Background L...

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Autores principales:	Mikhail Zarubin, Albert Gangapshev, Yuri Gavriljuk, Vladimir Kazalov, Elena Kravchenko
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fe2024faebe481a9ce971531ad954c9
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spelling	oai:doaj.org-article:7fe2024faebe481a9ce971531ad954c92021-12-02T20:15:13ZFirst transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.1932-620310.1371/journal.pone.0255066https://doaj.org/article/7fe2024faebe481a9ce971531ad954c92021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255066https://doaj.org/toc/1932-6203Natural background radiation is a permanent multicomponent factor. It has an influence on biological organisms, but effects of its deprivation still remain unclear. The aim of our work was to study for the first time responses of D. melanogaster to conditions of the Deep Underground Low-Background Laboratory DULB-4900 (BNO, INR, RAS, Russia) at the transcriptome level by RNA-seq profiling. Overall 77 transcripts demonstrated differential abundance between flies exposed to low and natural background radiation. Enriched biological process functional categories were established for all genes with differential expression. The results showed down-regulation of primary metabolic processes and up-regulation of both the immune system process and the response to stimuli. The comparative analysis of our data and publicly available transcriptome data on D. melanogaster exposed to low and high doses of ionizing radiation did not reveal common DEGs in them. We hypothesize that the observed changes in gene expression can be explained by the influence of the underground conditions in DULB-4900, in particular, by the lack of stimuli. Thus, our study challenges the validity of the LNT model for the region of background radiation doses below a certain level (~16.4 nGy h-1) and the presence of a dose threshold for D. melanogaster.Mikhail ZarubinAlbert GangapshevYuri GavriljukVladimir KazalovElena KravchenkoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255066 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mikhail Zarubin Albert Gangapshev Yuri Gavriljuk Vladimir Kazalov Elena Kravchenko First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.
description	Natural background radiation is a permanent multicomponent factor. It has an influence on biological organisms, but effects of its deprivation still remain unclear. The aim of our work was to study for the first time responses of D. melanogaster to conditions of the Deep Underground Low-Background Laboratory DULB-4900 (BNO, INR, RAS, Russia) at the transcriptome level by RNA-seq profiling. Overall 77 transcripts demonstrated differential abundance between flies exposed to low and natural background radiation. Enriched biological process functional categories were established for all genes with differential expression. The results showed down-regulation of primary metabolic processes and up-regulation of both the immune system process and the response to stimuli. The comparative analysis of our data and publicly available transcriptome data on D. melanogaster exposed to low and high doses of ionizing radiation did not reveal common DEGs in them. We hypothesize that the observed changes in gene expression can be explained by the influence of the underground conditions in DULB-4900, in particular, by the lack of stimuli. Thus, our study challenges the validity of the LNT model for the region of background radiation doses below a certain level (~16.4 nGy h-1) and the presence of a dose threshold for D. melanogaster.
format	article
author	Mikhail Zarubin Albert Gangapshev Yuri Gavriljuk Vladimir Kazalov Elena Kravchenko
author_facet	Mikhail Zarubin Albert Gangapshev Yuri Gavriljuk Vladimir Kazalov Elena Kravchenko
author_sort	Mikhail Zarubin
title	First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.
title_short	First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.
title_full	First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.
title_fullStr	First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.
title_full_unstemmed	First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.
title_sort	first transcriptome profiling of d. melanogaster after development in a deep underground low radiation background laboratory.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7fe2024faebe481a9ce971531ad954c9
work_keys_str_mv	AT mikhailzarubin firsttranscriptomeprofilingofdmelanogasterafterdevelopmentinadeepundergroundlowradiationbackgroundlaboratory AT albertgangapshev firsttranscriptomeprofilingofdmelanogasterafterdevelopmentinadeepundergroundlowradiationbackgroundlaboratory AT yurigavriljuk firsttranscriptomeprofilingofdmelanogasterafterdevelopmentinadeepundergroundlowradiationbackgroundlaboratory AT vladimirkazalov firsttranscriptomeprofilingofdmelanogasterafterdevelopmentinadeepundergroundlowradiationbackgroundlaboratory AT elenakravchenko firsttranscriptomeprofilingofdmelanogasterafterdevelopmentinadeepundergroundlowradiationbackgroundlaboratory
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First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.

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