DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.

Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the me...

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Autores principales:	Stefania Romeo, Luigi Zeni, Maurizio Sarti, Anna Sannino, Maria Rosaria Scarfì, P Thomas Vernier, Olga Zeni
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fe8758bbeab49af9c37e04e24fc3ada
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spelling	oai:doaj.org-article:7fe8758bbeab49af9c37e04e24fc3ada2021-11-18T07:33:06ZDNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.1932-620310.1371/journal.pone.0028419https://doaj.org/article/7fe8758bbeab49af9c37e04e24fc3ada2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22164287/?tool=EBIhttps://doaj.org/toc/1932-6203Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the mechanisms underlying such bioelectrical effects. In this work, we explored relatively mild exposure conditions to provide insight into weak, reversible effects, laying a foundation for a better understanding of the interaction mechanisms and kinetics underlying nsPEF bio-effects. In particular, we report changes in the nucleus of Jurkat cells (human lymphoblastoid T cells) exposed to single pulses of 60 ns duration and 1.0, 1.5 and 2.5 MV/m amplitudes, which do not affect cell growth and viability. A dose-dependent reduction in alkaline comet-assayed DNA migration is observed immediately after nsPEF exposure, accompanied by permeabilization of the plasma membrane (YO-PRO-1 uptake). Comet assay profiles return to normal within 60 minutes after pulse delivery at the highest pulse amplitude tested, indicating that our exposure protocol affects the nucleus, modifying DNA electrophoretic migration patterns.Stefania RomeoLuigi ZeniMaurizio SartiAnna SanninoMaria Rosaria ScarfìP Thomas VernierOlga ZeniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28419 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefania Romeo Luigi Zeni Maurizio Sarti Anna Sannino Maria Rosaria Scarfì P Thomas Vernier Olga Zeni DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.
description	Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the mechanisms underlying such bioelectrical effects. In this work, we explored relatively mild exposure conditions to provide insight into weak, reversible effects, laying a foundation for a better understanding of the interaction mechanisms and kinetics underlying nsPEF bio-effects. In particular, we report changes in the nucleus of Jurkat cells (human lymphoblastoid T cells) exposed to single pulses of 60 ns duration and 1.0, 1.5 and 2.5 MV/m amplitudes, which do not affect cell growth and viability. A dose-dependent reduction in alkaline comet-assayed DNA migration is observed immediately after nsPEF exposure, accompanied by permeabilization of the plasma membrane (YO-PRO-1 uptake). Comet assay profiles return to normal within 60 minutes after pulse delivery at the highest pulse amplitude tested, indicating that our exposure protocol affects the nucleus, modifying DNA electrophoretic migration patterns.
format	article
author	Stefania Romeo Luigi Zeni Maurizio Sarti Anna Sannino Maria Rosaria Scarfì P Thomas Vernier Olga Zeni
author_facet	Stefania Romeo Luigi Zeni Maurizio Sarti Anna Sannino Maria Rosaria Scarfì P Thomas Vernier Olga Zeni
author_sort	Stefania Romeo
title	DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.
title_short	DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.
title_full	DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.
title_fullStr	DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.
title_full_unstemmed	DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.
title_sort	dna electrophoretic migration patterns change after exposure of jurkat cells to a single intense nanosecond electric pulse.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7fe8758bbeab49af9c37e04e24fc3ada
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DNA electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse.

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