Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.

Trypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket...

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Autores principales:	Mélanie Bonhivers, Sophie Nowacki, Nicolas Landrein, Derrick R Robinson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7fe882aa5dda45fcbac74bdf134a32eb
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spelling	oai:doaj.org-article:7fe882aa5dda45fcbac74bdf134a32eb2021-11-25T05:33:22ZBiogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.1544-91731545-788510.1371/journal.pbio.0060105https://doaj.org/article/7fe882aa5dda45fcbac74bdf134a32eb2008-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18462016/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Trypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket (FP), an invagination of the pellicular membrane. The pocket is the sole site for specific receptors thus maintaining them inaccessible to components of the innate immune system of the mammalian host. The FP is also responsible for the sorting of protective parasite glycoproteins targeted to, or recycling from, the pellicular membrane, and for the removal of host antibodies from the cell surface. Here, we describe the first characterisation of a flagellar pocket cytoskeletal protein, BILBO1. BILBO1 functions to form a cytoskeleton framework upon which the FP is made and which is also required and essential for FP biogenesis and cell survival. Remarkably, RNA interference (RNAi)-mediated ablation of BILBO1 in insect procyclic-form parasites prevents FP biogenesis and induces vesicle accumulation, Golgi swelling, the aberrant repositioning of the new flagellum, and cell death. Cultured bloodstream-form parasites are also nonviable when subjected to BILBO1 RNAi. These results provide the first molecular evidence for cytoskeletally mediated FP biogenesis.Mélanie BonhiversSophie NowackiNicolas LandreinDerrick R RobinsonPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 5, p e105 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Mélanie Bonhivers Sophie Nowacki Nicolas Landrein Derrick R Robinson Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
description	Trypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket (FP), an invagination of the pellicular membrane. The pocket is the sole site for specific receptors thus maintaining them inaccessible to components of the innate immune system of the mammalian host. The FP is also responsible for the sorting of protective parasite glycoproteins targeted to, or recycling from, the pellicular membrane, and for the removal of host antibodies from the cell surface. Here, we describe the first characterisation of a flagellar pocket cytoskeletal protein, BILBO1. BILBO1 functions to form a cytoskeleton framework upon which the FP is made and which is also required and essential for FP biogenesis and cell survival. Remarkably, RNA interference (RNAi)-mediated ablation of BILBO1 in insect procyclic-form parasites prevents FP biogenesis and induces vesicle accumulation, Golgi swelling, the aberrant repositioning of the new flagellum, and cell death. Cultured bloodstream-form parasites are also nonviable when subjected to BILBO1 RNAi. These results provide the first molecular evidence for cytoskeletally mediated FP biogenesis.
format	article
author	Mélanie Bonhivers Sophie Nowacki Nicolas Landrein Derrick R Robinson
author_facet	Mélanie Bonhivers Sophie Nowacki Nicolas Landrein Derrick R Robinson
author_sort	Mélanie Bonhivers
title	Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
title_short	Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
title_full	Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
title_fullStr	Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
title_full_unstemmed	Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
title_sort	biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/7fe882aa5dda45fcbac74bdf134a32eb
work_keys_str_mv	AT melaniebonhivers biogenesisofthetrypanosomeendoexocytoticorganelleiscytoskeletonmediated AT sophienowacki biogenesisofthetrypanosomeendoexocytoticorganelleiscytoskeletonmediated AT nicolaslandrein biogenesisofthetrypanosomeendoexocytoticorganelleiscytoskeletonmediated AT derrickrrobinson biogenesisofthetrypanosomeendoexocytoticorganelleiscytoskeletonmediated
_version_	1718414646101147648

Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.

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