Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization

Yong Wang,1,* Rongfen Gao,2,* Jinpeng Li,3,* Shaotao Tang,1 Shuai Li,1 Qiangsong Tong,1 Shiwang Li1 1Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People’s Republic of China; 2Department of Rheu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang Y, Gao R, Li J, Tang S, Li S, Tong Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	circular rnas hepatocellular carcinoma exosomes macrophage polarization invasion. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7fee7af60536468e87c314ea599a6e74
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7fee7af60536468e87c314ea599a6e74
record_format	dspace
spelling	oai:doaj.org-article:7fee7af60536468e87c314ea599a6e742021-12-02T17:00:24ZDownregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization1178-2013https://doaj.org/article/7fee7af60536468e87c314ea599a6e742021-04-01T00:00:00Zhttps://www.dovepress.com/downregulation-of-hsacirc0074854-suppresses-the-migration-and-invasion-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yong Wang,1,* Rongfen Gao,2,* Jinpeng Li,3,* Shaotao Tang,1 Shuai Li,1 Qiangsong Tong,1 Shiwang Li1 1Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People’s Republic of China; 2Department of Rheumatology and Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People’s Republic of China; 3Department of Thyroid and Breast Surgery, Wuhan University Zhongnan Hospital, Wuhan, 430071, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yong WangDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei, 430022, People’s Republic of ChinaEmail wangyong_678@126.comBackground: Circular RNAs (circRNAs) have been identified as key factors in the development of hepatocellular carcinoma (HCC). However, the role and potential molecular mechanism of circRNAs in HCC remain largely unclear. In addition, exosomes are known as important messengers of the cross-talk between tumor cells and immune cells, while the role of extracellular circRNAs in the cell-to-cell communication of tumor cells and immune cells remains not unclear.Methods: The level of hsa_circ_0074854 in HCC cell lines and HCC cell-derived exosomes was assessed using RT-qPCR assay. In addition, CCK-8 and transwell assays were used to determine the viability, migration and invasion of HCC cells.Results: Hsa_circ_0074854 expression was upregulated in HCC tissues and HCC cell lines. Additionally, hsa_circ_0074854 knockdown was found to inhibit HCC growth in vitro and in vivo. Mechanistically, hsa_circ_0074854 knockdown inhibited the migration and invasion of HCC cells via interacting with human antigen R (HuR) to reduce its stability. Furthermore, hsa_circ_0074854 can be transferred from HCC cells to macrophages via exosomes. Exosomes with downregulated hsa_circ_0074854 suppressed macrophage M2 polarization, which in turn suppressing migration and invasion of HCC cells both in vitro and in vivo.Conclusion: Downregulation of hsa_circ_0074854 suppresses the migration and invasion in hepatocellular carcinoma via interacting with HuR and via suppressing exosomes-mediated macrophage M2 polarization. Collectively, these findings may help to understand the diagnosis and treatment of HCC.Keywords: circular RNAs, hepatocellular carcinoma, exosomes, macrophage polarization, invasionWang YGao RLi JTang SLi STong QLi SDove Medical Pressarticlecircular rnashepatocellular carcinomaexosomesmacrophage polarizationinvasion.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 2803-2818 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circular rnas hepatocellular carcinoma exosomes macrophage polarization invasion. Medicine (General) R5-920
spellingShingle	circular rnas hepatocellular carcinoma exosomes macrophage polarization invasion. Medicine (General) R5-920 Wang Y Gao R Li J Tang S Li S Tong Q Li S Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization
description	Yong Wang,1,* Rongfen Gao,2,* Jinpeng Li,3,* Shaotao Tang,1 Shuai Li,1 Qiangsong Tong,1 Shiwang Li1 1Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People’s Republic of China; 2Department of Rheumatology and Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People’s Republic of China; 3Department of Thyroid and Breast Surgery, Wuhan University Zhongnan Hospital, Wuhan, 430071, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yong WangDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei, 430022, People’s Republic of ChinaEmail wangyong_678@126.comBackground: Circular RNAs (circRNAs) have been identified as key factors in the development of hepatocellular carcinoma (HCC). However, the role and potential molecular mechanism of circRNAs in HCC remain largely unclear. In addition, exosomes are known as important messengers of the cross-talk between tumor cells and immune cells, while the role of extracellular circRNAs in the cell-to-cell communication of tumor cells and immune cells remains not unclear.Methods: The level of hsa_circ_0074854 in HCC cell lines and HCC cell-derived exosomes was assessed using RT-qPCR assay. In addition, CCK-8 and transwell assays were used to determine the viability, migration and invasion of HCC cells.Results: Hsa_circ_0074854 expression was upregulated in HCC tissues and HCC cell lines. Additionally, hsa_circ_0074854 knockdown was found to inhibit HCC growth in vitro and in vivo. Mechanistically, hsa_circ_0074854 knockdown inhibited the migration and invasion of HCC cells via interacting with human antigen R (HuR) to reduce its stability. Furthermore, hsa_circ_0074854 can be transferred from HCC cells to macrophages via exosomes. Exosomes with downregulated hsa_circ_0074854 suppressed macrophage M2 polarization, which in turn suppressing migration and invasion of HCC cells both in vitro and in vivo.Conclusion: Downregulation of hsa_circ_0074854 suppresses the migration and invasion in hepatocellular carcinoma via interacting with HuR and via suppressing exosomes-mediated macrophage M2 polarization. Collectively, these findings may help to understand the diagnosis and treatment of HCC.Keywords: circular RNAs, hepatocellular carcinoma, exosomes, macrophage polarization, invasion
format	article
author	Wang Y Gao R Li J Tang S Li S Tong Q Li S
author_facet	Wang Y Gao R Li J Tang S Li S Tong Q Li S
author_sort	Wang Y
title	Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization
title_short	Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization
title_full	Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization
title_fullStr	Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization
title_full_unstemmed	Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization
title_sort	downregulation of hsa_circ_0074854 suppresses the migration and invasion in hepatocellular carcinoma via interacting with hur and via suppressing exosomes-mediated macrophage m2 polarization
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/7fee7af60536468e87c314ea599a6e74
work_keys_str_mv	AT wangy downregulationofhsacirc0074854suppressesthemigrationandinvasioninhepatocellularcarcinomaviainteractingwithhurandviasuppressingexosomesmediatedmacrophagem2polarization AT gaor downregulationofhsacirc0074854suppressesthemigrationandinvasioninhepatocellularcarcinomaviainteractingwithhurandviasuppressingexosomesmediatedmacrophagem2polarization AT lij downregulationofhsacirc0074854suppressesthemigrationandinvasioninhepatocellularcarcinomaviainteractingwithhurandviasuppressingexosomesmediatedmacrophagem2polarization AT tangs downregulationofhsacirc0074854suppressesthemigrationandinvasioninhepatocellularcarcinomaviainteractingwithhurandviasuppressingexosomesmediatedmacrophagem2polarization AT lis downregulationofhsacirc0074854suppressesthemigrationandinvasioninhepatocellularcarcinomaviainteractingwithhurandviasuppressingexosomesmediatedmacrophagem2polarization AT tongq downregulationofhsacirc0074854suppressesthemigrationandinvasioninhepatocellularcarcinomaviainteractingwithhurandviasuppressingexosomesmediatedmacrophagem2polarization AT lis downregulationofhsacirc0074854suppressesthemigrationandinvasioninhepatocellularcarcinomaviainteractingwithhurandviasuppressingexosomesmediatedmacrophagem2polarization
_version_	1718382219671633920

Downregulation of hsa_circ_0074854 Suppresses the Migration and Invasion in Hepatocellular Carcinoma via Interacting with HuR and via Suppressing Exosomes-Mediated Macrophage M2 Polarization

Ejemplares similares