Transcriptional activators of human genes with programmable DNA-specificity.

TAL (transcription activator-like) effectors are translocated by Xanthomonas bacteria into plant cells where they activate transcription of target genes. DNA target sequence recognition occurs in a unique mode involving a central domain of tandem repeats. Each repeat recognizes a single base pair in...

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Autores principales:	René Geissler, Heidi Scholze, Simone Hahn, Jana Streubel, Ulla Bonas, Sven-Erik Behrens, Jens Boch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ff6afeaea36486f9008c321137fc741
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spelling	oai:doaj.org-article:7ff6afeaea36486f9008c321137fc7412021-11-18T06:53:37ZTranscriptional activators of human genes with programmable DNA-specificity.1932-620310.1371/journal.pone.0019509https://doaj.org/article/7ff6afeaea36486f9008c321137fc7412011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625585/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203TAL (transcription activator-like) effectors are translocated by Xanthomonas bacteria into plant cells where they activate transcription of target genes. DNA target sequence recognition occurs in a unique mode involving a central domain of tandem repeats. Each repeat recognizes a single base pair in a contiguous DNA sequence and a pair of adjacent hypervariable amino acid residues per repeat specifies which base is bound. Rearranging the repeats allows the design of novel TAL proteins with predictable DNA-recognition specificities. TAL protein-based transcriptional activation in plant cells is mediated by a C-terminal activation domain (AD). Here, we created synthetic TAL proteins with designed repeat compositions using a novel modular cloning strategy termed "Golden TAL Technology". Newly programmed TAL proteins were not only functional in plant cells, but also in human cells and activated targeted expression of exogenous as well as endogenous genes. Transcriptional activation in different human cell lines was markedly improved by replacing the TAL-AD with the VP16-AD of herpes simplex virus. The creation of TAL proteins with potentially any desired DNA-recognition specificity allows their versatile use in biotechnology.René GeisslerHeidi ScholzeSimone HahnJana StreubelUlla BonasSven-Erik BehrensJens BochPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19509 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q René Geissler Heidi Scholze Simone Hahn Jana Streubel Ulla Bonas Sven-Erik Behrens Jens Boch Transcriptional activators of human genes with programmable DNA-specificity.
description	TAL (transcription activator-like) effectors are translocated by Xanthomonas bacteria into plant cells where they activate transcription of target genes. DNA target sequence recognition occurs in a unique mode involving a central domain of tandem repeats. Each repeat recognizes a single base pair in a contiguous DNA sequence and a pair of adjacent hypervariable amino acid residues per repeat specifies which base is bound. Rearranging the repeats allows the design of novel TAL proteins with predictable DNA-recognition specificities. TAL protein-based transcriptional activation in plant cells is mediated by a C-terminal activation domain (AD). Here, we created synthetic TAL proteins with designed repeat compositions using a novel modular cloning strategy termed "Golden TAL Technology". Newly programmed TAL proteins were not only functional in plant cells, but also in human cells and activated targeted expression of exogenous as well as endogenous genes. Transcriptional activation in different human cell lines was markedly improved by replacing the TAL-AD with the VP16-AD of herpes simplex virus. The creation of TAL proteins with potentially any desired DNA-recognition specificity allows their versatile use in biotechnology.
format	article
author	René Geissler Heidi Scholze Simone Hahn Jana Streubel Ulla Bonas Sven-Erik Behrens Jens Boch
author_facet	René Geissler Heidi Scholze Simone Hahn Jana Streubel Ulla Bonas Sven-Erik Behrens Jens Boch
author_sort	René Geissler
title	Transcriptional activators of human genes with programmable DNA-specificity.
title_short	Transcriptional activators of human genes with programmable DNA-specificity.
title_full	Transcriptional activators of human genes with programmable DNA-specificity.
title_fullStr	Transcriptional activators of human genes with programmable DNA-specificity.
title_full_unstemmed	Transcriptional activators of human genes with programmable DNA-specificity.
title_sort	transcriptional activators of human genes with programmable dna-specificity.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7ff6afeaea36486f9008c321137fc741
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Transcriptional activators of human genes with programmable DNA-specificity.

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