Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation

The carcinogenicity of radon has been convincingly documented through epidemiological studies of underground miners. However, there is a lack of early warning indicators for radon radiation damage. In this study, mixed serum samples of 3 groups were collected from 27 underground uranium miners and s...

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Autores principales:	Xuhong Dang, Haipeng Lin, Yayi Yuan, Biao Yang, Juancong Dong, Zhongxin Zhang, Kai Yang, Youchen Li, Xiaozhen Li, Dongliang Chai, Yahui Zuo
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7ff86e4a43e345008d548946face1835
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spelling	oai:doaj.org-article:7ff86e4a43e345008d548946face18352021-11-21T02:03:32ZQuantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation1559-325810.1177/15593258211056190https://doaj.org/article/7ff86e4a43e345008d548946face18352021-10-01T00:00:00Zhttps://doi.org/10.1177/15593258211056190https://doaj.org/toc/1559-3258The carcinogenicity of radon has been convincingly documented through epidemiological studies of underground miners. However, there is a lack of early warning indicators for radon radiation damage. In this study, mixed serum samples of 3 groups were collected from 27 underground uranium miners and seven aboveground miners according to the radiation exposure dose. The differentially expressed proteins in the serum were identified using the isobaric tags for the relative and absolute quantitation (iTRAQ)-based method. Some differentially expressed proteins were validated by enzyme-linked immunosorbent assay (ELISA) in 84 underground and 32 aboveground miners. A total of 25 co-differentially expressed proteins in 2 underground miner groups were screened, of which 9 were downregulated and 13 were upregulated. Biological process analysis of these proteins using Metascape showed that 5 GO terms were enriched, such as negative regulation of very-low-density lipoprotein particle clearance, endocytosis, and regulated exocytosis. The results of the ELISA for the expression levels of GCN1, CIP2A, and IGHV1-24 in the serum of 116 miners’ serum showed that the levels of GCN1 and CIP2A were consistent with the iTRAQ results. In conclusion, APOC1, APOC2, APOC3, ORM1, ORM2, ANTXR1, GCN1, and CIP2A may be potential early markers of radon radiation damage.Xuhong DangHaipeng LinYayi YuanBiao YangJuancong DongZhongxin ZhangKai YangYouchen LiXiaozhen LiDongliang ChaiYahui ZuoSAGE PublishingarticleTherapeutics. PharmacologyRM1-950ENDose-Response, Vol 19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950
spellingShingle	Therapeutics. Pharmacology RM1-950 Xuhong Dang Haipeng Lin Yayi Yuan Biao Yang Juancong Dong Zhongxin Zhang Kai Yang Youchen Li Xiaozhen Li Dongliang Chai Yahui Zuo Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation
description	The carcinogenicity of radon has been convincingly documented through epidemiological studies of underground miners. However, there is a lack of early warning indicators for radon radiation damage. In this study, mixed serum samples of 3 groups were collected from 27 underground uranium miners and seven aboveground miners according to the radiation exposure dose. The differentially expressed proteins in the serum were identified using the isobaric tags for the relative and absolute quantitation (iTRAQ)-based method. Some differentially expressed proteins were validated by enzyme-linked immunosorbent assay (ELISA) in 84 underground and 32 aboveground miners. A total of 25 co-differentially expressed proteins in 2 underground miner groups were screened, of which 9 were downregulated and 13 were upregulated. Biological process analysis of these proteins using Metascape showed that 5 GO terms were enriched, such as negative regulation of very-low-density lipoprotein particle clearance, endocytosis, and regulated exocytosis. The results of the ELISA for the expression levels of GCN1, CIP2A, and IGHV1-24 in the serum of 116 miners’ serum showed that the levels of GCN1 and CIP2A were consistent with the iTRAQ results. In conclusion, APOC1, APOC2, APOC3, ORM1, ORM2, ANTXR1, GCN1, and CIP2A may be potential early markers of radon radiation damage.
format	article
author	Xuhong Dang Haipeng Lin Yayi Yuan Biao Yang Juancong Dong Zhongxin Zhang Kai Yang Youchen Li Xiaozhen Li Dongliang Chai Yahui Zuo
author_facet	Xuhong Dang Haipeng Lin Yayi Yuan Biao Yang Juancong Dong Zhongxin Zhang Kai Yang Youchen Li Xiaozhen Li Dongliang Chai Yahui Zuo
author_sort	Xuhong Dang
title	Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation
title_short	Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation
title_full	Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation
title_fullStr	Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation
title_full_unstemmed	Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation
title_sort	quantitative proteomics analysis of differentially expressed proteins in serum of former uranium miners by isobaric tags for the relative and absolute quantitation
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/7ff86e4a43e345008d548946face1835
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Quantitative Proteomics Analysis of Differentially Expressed Proteins in Serum of Former Uranium Miners by Isobaric Tags for the Relative and Absolute Quantitation

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