Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells

The aim of our study was to investigate the effect of three lignans (schisandrol A, schisandrol B, and schisandrin C) on insulin secretion in rat INS-1 pancreatic β-cells and glucose uptake in mouse C2C12 skeletal muscle cells. Schisandrol A and schisandrin C enhanced insulin secretion in response t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dahae Lee, Young-Mi Kim, Hyun Woo Kim, You-Kyoung Choi, Bang Ju Park, Sang Hoon Joo, Ki Sung Kang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	schisandrin C glucose-stimulated insulin secretion glucose uptake PDX-1 GLUT-4 Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/7ffd087582584124af668994bd5dc60b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7ffd087582584124af668994bd5dc60b
record_format	dspace
spelling	oai:doaj.org-article:7ffd087582584124af668994bd5dc60b2021-11-11T18:30:17ZSchisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells10.3390/molecules262165091420-3049https://doaj.org/article/7ffd087582584124af668994bd5dc60b2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6509https://doaj.org/toc/1420-3049The aim of our study was to investigate the effect of three lignans (schisandrol A, schisandrol B, and schisandrin C) on insulin secretion in rat INS-1 pancreatic β-cells and glucose uptake in mouse C2C12 skeletal muscle cells. Schisandrol A and schisandrin C enhanced insulin secretion in response to high glucose levels with no toxic effects on INS-1 cells. The effect of schisandrin C was superior to that of gliclazide (positive control), a drug commonly used to treat type 2 diabetes (T2D). In addition, western blot analysis showed that the expression of associated proteins, including peroxisome proliferator-activated receptor γ (PPARγ), pancreatic and duodenal homeobox 1 (PDX-1), phosphatidylinositol 3-kinase (PI3K), Akt, and insulin receptor substrate-2 (IRS-2), was increased in INS-1 cells after treatment with schisandrin C. In addition, insulin secretion effect of schisandrin C were enhanced by the Bay K 8644 (L-type Ca<sup>2+</sup> channel agonist) and glibenclamide (K<sup>+</sup> channel blocker), were abolished by the nifedipine (L-type Ca<sup>2+</sup> channel blocker) and diazoxide (K<sup>+</sup> channel activator). Moreover, schisandrin C enhanced glucose uptake with no toxic effects on C2C12 cells. Western blot analysis showed that the expression of associated proteins, including insulin receptor substrate-1 (IRS-1), AMP-activated protein kinase (AMPK), PI3K, Akt, glucose transporter type 4 (GLUT-4), was increased in C2C12 cells after treatment with schisandrin C. Schisandrin C may improve hyperglycemia by enhancing insulin secretion in pancreatic β-cells and improving glucose uptake into skeletal muscle cells. Our findings may provide evidence that schisandrin C may be beneficial in devising novel anti-T2D strategies.Dahae LeeYoung-Mi KimHyun Woo KimYou-Kyoung ChoiBang Ju ParkSang Hoon JooKi Sung KangMDPI AGarticleschisandrin Cglucose-stimulated insulin secretionglucose uptakePDX-1GLUT-4Organic chemistryQD241-441ENMolecules, Vol 26, Iss 6509, p 6509 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	schisandrin C glucose-stimulated insulin secretion glucose uptake PDX-1 GLUT-4 Organic chemistry QD241-441
spellingShingle	schisandrin C glucose-stimulated insulin secretion glucose uptake PDX-1 GLUT-4 Organic chemistry QD241-441 Dahae Lee Young-Mi Kim Hyun Woo Kim You-Kyoung Choi Bang Ju Park Sang Hoon Joo Ki Sung Kang Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells
description	The aim of our study was to investigate the effect of three lignans (schisandrol A, schisandrol B, and schisandrin C) on insulin secretion in rat INS-1 pancreatic β-cells and glucose uptake in mouse C2C12 skeletal muscle cells. Schisandrol A and schisandrin C enhanced insulin secretion in response to high glucose levels with no toxic effects on INS-1 cells. The effect of schisandrin C was superior to that of gliclazide (positive control), a drug commonly used to treat type 2 diabetes (T2D). In addition, western blot analysis showed that the expression of associated proteins, including peroxisome proliferator-activated receptor γ (PPARγ), pancreatic and duodenal homeobox 1 (PDX-1), phosphatidylinositol 3-kinase (PI3K), Akt, and insulin receptor substrate-2 (IRS-2), was increased in INS-1 cells after treatment with schisandrin C. In addition, insulin secretion effect of schisandrin C were enhanced by the Bay K 8644 (L-type Ca<sup>2+</sup> channel agonist) and glibenclamide (K<sup>+</sup> channel blocker), were abolished by the nifedipine (L-type Ca<sup>2+</sup> channel blocker) and diazoxide (K<sup>+</sup> channel activator). Moreover, schisandrin C enhanced glucose uptake with no toxic effects on C2C12 cells. Western blot analysis showed that the expression of associated proteins, including insulin receptor substrate-1 (IRS-1), AMP-activated protein kinase (AMPK), PI3K, Akt, glucose transporter type 4 (GLUT-4), was increased in C2C12 cells after treatment with schisandrin C. Schisandrin C may improve hyperglycemia by enhancing insulin secretion in pancreatic β-cells and improving glucose uptake into skeletal muscle cells. Our findings may provide evidence that schisandrin C may be beneficial in devising novel anti-T2D strategies.
format	article
author	Dahae Lee Young-Mi Kim Hyun Woo Kim You-Kyoung Choi Bang Ju Park Sang Hoon Joo Ki Sung Kang
author_facet	Dahae Lee Young-Mi Kim Hyun Woo Kim You-Kyoung Choi Bang Ju Park Sang Hoon Joo Ki Sung Kang
author_sort	Dahae Lee
title	Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells
title_short	Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells
title_full	Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells
title_fullStr	Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells
title_full_unstemmed	Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells
title_sort	schisandrin c affects glucose-stimulated insulin secretion in pancreatic β-cells and glucose uptake in skeletal muscle cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7ffd087582584124af668994bd5dc60b
work_keys_str_mv	AT dahaelee schisandrincaffectsglucosestimulatedinsulinsecretioninpancreaticbcellsandglucoseuptakeinskeletalmusclecells AT youngmikim schisandrincaffectsglucosestimulatedinsulinsecretioninpancreaticbcellsandglucoseuptakeinskeletalmusclecells AT hyunwookim schisandrincaffectsglucosestimulatedinsulinsecretioninpancreaticbcellsandglucoseuptakeinskeletalmusclecells AT youkyoungchoi schisandrincaffectsglucosestimulatedinsulinsecretioninpancreaticbcellsandglucoseuptakeinskeletalmusclecells AT bangjupark schisandrincaffectsglucosestimulatedinsulinsecretioninpancreaticbcellsandglucoseuptakeinskeletalmusclecells AT sanghoonjoo schisandrincaffectsglucosestimulatedinsulinsecretioninpancreaticbcellsandglucoseuptakeinskeletalmusclecells AT kisungkang schisandrincaffectsglucosestimulatedinsulinsecretioninpancreaticbcellsandglucoseuptakeinskeletalmusclecells
_version_	1718431831521492992

Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells

Ejemplares similares