Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.

Activation-induced cytidine deminase (Aid), a unique enzyme that deaminates cytosine in DNA, shuttles between the nucleus and the cytoplasm. A recent study proposed a novel function of Aid in active DNA demethylation via deamination of 5-hydroxymethylcytosine, which is converted from 5-methylcytosin...

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Autores principales:	Yuko Arioka, Akira Watanabe, Kuniaki Saito, Yasuhiro Yamada
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/800022fb773949099c6f01821e33d4e8
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spelling	oai:doaj.org-article:800022fb773949099c6f01821e33d4e82021-11-25T06:10:27ZActivation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.1932-620310.1371/journal.pone.0045031https://doaj.org/article/800022fb773949099c6f01821e33d4e82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028748/?tool=EBIhttps://doaj.org/toc/1932-6203Activation-induced cytidine deminase (Aid), a unique enzyme that deaminates cytosine in DNA, shuttles between the nucleus and the cytoplasm. A recent study proposed a novel function of Aid in active DNA demethylation via deamination of 5-hydroxymethylcytosine, which is converted from 5-methylcytosine by the Ten-eleven translocation (Tet) family of enzymes. In this study, we examined the effect of simultaneous expression of Aid and Tet family proteins on the subcellular localization of each protein. We found that overexpressed Aid is mainly localized in the cytoplasm, whereas Tet1 and Tet2 are localized in the nucleus, and Tet3 is localized in both the cytoplasm and the nucleus. However, nuclear Tet proteins were gradually translocated to the cytoplasm when co-expressed with Aid. We also show that Aid-mediated translocation of Tet proteins is associated with Aid shuttling. Here we propose a possible role for Aid as a regulator of the subcellular localization of Tet family proteins.Yuko AriokaAkira WatanabeKuniaki SaitoYasuhiro YamadaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45031 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuko Arioka Akira Watanabe Kuniaki Saito Yasuhiro Yamada Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.
description	Activation-induced cytidine deminase (Aid), a unique enzyme that deaminates cytosine in DNA, shuttles between the nucleus and the cytoplasm. A recent study proposed a novel function of Aid in active DNA demethylation via deamination of 5-hydroxymethylcytosine, which is converted from 5-methylcytosine by the Ten-eleven translocation (Tet) family of enzymes. In this study, we examined the effect of simultaneous expression of Aid and Tet family proteins on the subcellular localization of each protein. We found that overexpressed Aid is mainly localized in the cytoplasm, whereas Tet1 and Tet2 are localized in the nucleus, and Tet3 is localized in both the cytoplasm and the nucleus. However, nuclear Tet proteins were gradually translocated to the cytoplasm when co-expressed with Aid. We also show that Aid-mediated translocation of Tet proteins is associated with Aid shuttling. Here we propose a possible role for Aid as a regulator of the subcellular localization of Tet family proteins.
format	article
author	Yuko Arioka Akira Watanabe Kuniaki Saito Yasuhiro Yamada
author_facet	Yuko Arioka Akira Watanabe Kuniaki Saito Yasuhiro Yamada
author_sort	Yuko Arioka
title	Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.
title_short	Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.
title_full	Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.
title_fullStr	Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.
title_full_unstemmed	Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.
title_sort	activation-induced cytidine deaminase alters the subcellular localization of tet family proteins.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/800022fb773949099c6f01821e33d4e8
work_keys_str_mv	AT yukoarioka activationinducedcytidinedeaminasealtersthesubcellularlocalizationoftetfamilyproteins AT akirawatanabe activationinducedcytidinedeaminasealtersthesubcellularlocalizationoftetfamilyproteins AT kuniakisaito activationinducedcytidinedeaminasealtersthesubcellularlocalizationoftetfamilyproteins AT yasuhiroyamada activationinducedcytidinedeaminasealtersthesubcellularlocalizationoftetfamilyproteins
_version_	1718414111654543360

Activation-induced cytidine deaminase alters the subcellular localization of Tet family proteins.

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