UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

Background Osteoporosis has gradually become a public health problem in the world. However, the exact molecular mechanism of osteoporosis still remains unclear. Senescence and osteogenic differentiation inhibition of bone marrow mesenchymal stem cells (BMSCs ) are supposed to play an important part...

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Autores principales:	Yalin Liu, Guangping Cai, Peng Chen, Tiejian Jiang, Zhuying Xia
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	UBE2E3 Osteoporosis Osteogenic differentiation Senescence Nrf2 Medicine R
Acceso en línea:	https://doaj.org/article/8005adf9ae834890bb5a191d5798d839
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spelling	oai:doaj.org-article:8005adf9ae834890bb5a191d5798d8392021-11-20T15:05:05ZUBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging10.7717/peerj.122532167-8359https://doaj.org/article/8005adf9ae834890bb5a191d5798d8392021-11-01T00:00:00Zhttps://peerj.com/articles/12253.pdfhttps://peerj.com/articles/12253/https://doaj.org/toc/2167-8359Background Osteoporosis has gradually become a public health problem in the world. However, the exact molecular mechanism of osteoporosis still remains unclear. Senescence and osteogenic differentiation inhibition of bone marrow mesenchymal stem cells (BMSCs ) are supposed to play an important part in osteoporosis. Methods We used two gene expression profiles (GSE35956 and GSE35958) associated with osteoporosis and selected the promising gene Ubiquitin-conjugating enzyme E2 E3 (UBE2E3). We then verified its function and mechanism by in vitro experiments. Results UBE2E3 was highly expressed in the bone marrow and positively associated with osteogenesis related genes. Besides, UBE2E3 expression reduced in old BMSCs compared with that in young BMSCs. In in vitro experiments, knockdown of UBE2E3 accelerated cellular senescence and inhibited osteogenic differentiation of young BMSCs. On the other hand, overexpression of UBE2E3 attenuated cellular senescence as well as enhanced osteogenic differentiation of old BMSCs. Mechanistically, UBE2E3 might regulate the nuclear factor erythroid 2-related factor (Nrf2) and control its function, thus affecting the senescence and osteogenic differentiation of BMSCs. Conclusion UBE2E3 may be potentially involved in the pathogenesis of osteoporosis by regulating cellular senescence and osteogenic differentiation of BMSCs.Yalin LiuGuangping CaiPeng ChenTiejian JiangZhuying XiaPeerJ Inc.articleUBE2E3OsteoporosisOsteogenic differentiationSenescenceNrf2MedicineRENPeerJ, Vol 9, p e12253 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	UBE2E3 Osteoporosis Osteogenic differentiation Senescence Nrf2 Medicine R
spellingShingle	UBE2E3 Osteoporosis Osteogenic differentiation Senescence Nrf2 Medicine R Yalin Liu Guangping Cai Peng Chen Tiejian Jiang Zhuying Xia UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging
description	Background Osteoporosis has gradually become a public health problem in the world. However, the exact molecular mechanism of osteoporosis still remains unclear. Senescence and osteogenic differentiation inhibition of bone marrow mesenchymal stem cells (BMSCs ) are supposed to play an important part in osteoporosis. Methods We used two gene expression profiles (GSE35956 and GSE35958) associated with osteoporosis and selected the promising gene Ubiquitin-conjugating enzyme E2 E3 (UBE2E3). We then verified its function and mechanism by in vitro experiments. Results UBE2E3 was highly expressed in the bone marrow and positively associated with osteogenesis related genes. Besides, UBE2E3 expression reduced in old BMSCs compared with that in young BMSCs. In in vitro experiments, knockdown of UBE2E3 accelerated cellular senescence and inhibited osteogenic differentiation of young BMSCs. On the other hand, overexpression of UBE2E3 attenuated cellular senescence as well as enhanced osteogenic differentiation of old BMSCs. Mechanistically, UBE2E3 might regulate the nuclear factor erythroid 2-related factor (Nrf2) and control its function, thus affecting the senescence and osteogenic differentiation of BMSCs. Conclusion UBE2E3 may be potentially involved in the pathogenesis of osteoporosis by regulating cellular senescence and osteogenic differentiation of BMSCs.
format	article
author	Yalin Liu Guangping Cai Peng Chen Tiejian Jiang Zhuying Xia
author_facet	Yalin Liu Guangping Cai Peng Chen Tiejian Jiang Zhuying Xia
author_sort	Yalin Liu
title	UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging
title_short	UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging
title_full	UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging
title_fullStr	UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging
title_full_unstemmed	UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging
title_sort	ube2e3 regulates cellular senescence and osteogenic differentiation of bmscs during aging
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/8005adf9ae834890bb5a191d5798d839
work_keys_str_mv	AT yalinliu ube2e3regulatescellularsenescenceandosteogenicdifferentiationofbmscsduringaging AT guangpingcai ube2e3regulatescellularsenescenceandosteogenicdifferentiationofbmscsduringaging AT pengchen ube2e3regulatescellularsenescenceandosteogenicdifferentiationofbmscsduringaging AT tiejianjiang ube2e3regulatescellularsenescenceandosteogenicdifferentiationofbmscsduringaging AT zhuyingxia ube2e3regulatescellularsenescenceandosteogenicdifferentiationofbmscsduringaging
_version_	1718419433795354624

UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

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