Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults

Abstract Robust biological markers of dietary exposure are essential in improving the understanding of the link between diet and health outcomes. Polyphenolic compounds, including flavonoids, have been proposed to mitigate the risk of chronic diseases where oxidative stress and inflammation play a c...

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Autores principales:	David Charles, Lee A. Gethings, James F. Potts, Peter G. J. Burney, Vanessa Garcia-Larsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8006c4dc784947648bcb93b26fdbeb8b
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spelling	oai:doaj.org-article:8006c4dc784947648bcb93b26fdbeb8b2021-12-02T15:53:45ZMass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults10.1038/s41598-021-85190-w2045-2322https://doaj.org/article/8006c4dc784947648bcb93b26fdbeb8b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85190-whttps://doaj.org/toc/2045-2322Abstract Robust biological markers of dietary exposure are essential in improving the understanding of the link between diet and health outcomes. Polyphenolic compounds, including flavonoids, have been proposed to mitigate the risk of chronic diseases where oxidative stress and inflammation play a central role. Biomarkers can provide objective measurement of the levels of polyphenolic compounds. In this study, we provide methodology to identify potential candidate markers of polyphenol intake in human serum. Seventeen participants from the UK arm of the Global Allergy and Asthma Network of Excellence (GA2LEN) had their dietary intake estimated using a validated food frequency questionnaire, and serum samples were assessed using mass spectrometry to identify potential candidate markers. 144 features were assigned identities, of these we identified four biologically relevant compounds (rhamnazin 3-rutinoside, 2-galloyl-1,4-galactarolactone methyl ester, 2″,32″-di-O-p-coumaroylafzelin and cyclocommunin), which were significantly increased in the serum of participants with high predicted level of fruit and vegetable intake. 2-galloyl-1,4-galactarolactone methyl ester was strongly correlated with total flavonoids (r = 0.62; P = 0.005), flavan-3-ols (r = 0.67; P = 0.002) as well as with other four subclasses. Rhamnazin 3-rutinoside showed strong correlation with pro-anthocyanidins (r = 0.68; P = 0.001), flavones (r = 0.62; P = 0.005). Our results suggest that serum profiling for these compounds might be an effective way of establishing the relative intake of flavonoids and could contribute to improve the accuracy of epidemiological methods to ascertain flavonoid intake.David CharlesLee A. GethingsJames F. PottsPeter G. J. BurneyVanessa Garcia-LarsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Charles Lee A. Gethings James F. Potts Peter G. J. Burney Vanessa Garcia-Larsen Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults
description	Abstract Robust biological markers of dietary exposure are essential in improving the understanding of the link between diet and health outcomes. Polyphenolic compounds, including flavonoids, have been proposed to mitigate the risk of chronic diseases where oxidative stress and inflammation play a central role. Biomarkers can provide objective measurement of the levels of polyphenolic compounds. In this study, we provide methodology to identify potential candidate markers of polyphenol intake in human serum. Seventeen participants from the UK arm of the Global Allergy and Asthma Network of Excellence (GA2LEN) had their dietary intake estimated using a validated food frequency questionnaire, and serum samples were assessed using mass spectrometry to identify potential candidate markers. 144 features were assigned identities, of these we identified four biologically relevant compounds (rhamnazin 3-rutinoside, 2-galloyl-1,4-galactarolactone methyl ester, 2″,32″-di-O-p-coumaroylafzelin and cyclocommunin), which were significantly increased in the serum of participants with high predicted level of fruit and vegetable intake. 2-galloyl-1,4-galactarolactone methyl ester was strongly correlated with total flavonoids (r = 0.62; P = 0.005), flavan-3-ols (r = 0.67; P = 0.002) as well as with other four subclasses. Rhamnazin 3-rutinoside showed strong correlation with pro-anthocyanidins (r = 0.68; P = 0.001), flavones (r = 0.62; P = 0.005). Our results suggest that serum profiling for these compounds might be an effective way of establishing the relative intake of flavonoids and could contribute to improve the accuracy of epidemiological methods to ascertain flavonoid intake.
format	article
author	David Charles Lee A. Gethings James F. Potts Peter G. J. Burney Vanessa Garcia-Larsen
author_facet	David Charles Lee A. Gethings James F. Potts Peter G. J. Burney Vanessa Garcia-Larsen
author_sort	David Charles
title	Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults
title_short	Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults
title_full	Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults
title_fullStr	Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults
title_full_unstemmed	Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults
title_sort	mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8006c4dc784947648bcb93b26fdbeb8b
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Mass spectrometry-based metabolomics for the discovery of candidate markers of flavonoid and polyphenolic intake in adults

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