The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.

GTPases of the Rho family are important molecular switches that regulate many basic cellular processes. The function of the Rho2 and Rho5 proteins from Saccharomyces cerevisiae and of their homologs in other species is poorly understood. Here, we report on the analysis of the AgRho2 and AgRho5 prote...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Doris Nordmann, Manuela Lickfeld, Verena Warnsmann, Johanna Wiechert, Arne Jendretzki, Hans-Peter Schmitz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/800c0a527a204196a1ad4e3b4f4e0535
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:800c0a527a204196a1ad4e3b4f4e0535
record_format	dspace
spelling	oai:doaj.org-article:800c0a527a204196a1ad4e3b4f4e05352021-11-25T06:02:43ZThe small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.1932-620310.1371/journal.pone.0106236https://doaj.org/article/800c0a527a204196a1ad4e3b4f4e05352014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25171205/?tool=EBIhttps://doaj.org/toc/1932-6203GTPases of the Rho family are important molecular switches that regulate many basic cellular processes. The function of the Rho2 and Rho5 proteins from Saccharomyces cerevisiae and of their homologs in other species is poorly understood. Here, we report on the analysis of the AgRho2 and AgRho5 proteins of the filamentous fungus Ashbya gossypii. In contrast to S. cerevisiae mutants of both encoding genes displayed a strong morphological phenotype. The Agrho2 mutants showed defects in tip-branching, while Agrho5 mutants had a significantly decreased growth rate and failed to maintain their growth axis. In addition, the Agrho5 mutants had highly defective actin rings at septation sites. We also found that a deletion mutant of a putative GDP-GTP-exchange factor (GEF) that was homologous to a Rac-GEF from other species phenocopied the Agrho5 mutant, suggesting that both proteins act in the same pathway, but the AgRho5 protein has acquired functions that are fulfilled by Rac-proteins in other species.Doris NordmannManuela LickfeldVerena WarnsmannJohanna WiechertArne JendretzkiHans-Peter SchmitzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e106236 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Doris Nordmann Manuela Lickfeld Verena Warnsmann Johanna Wiechert Arne Jendretzki Hans-Peter Schmitz The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.
description	GTPases of the Rho family are important molecular switches that regulate many basic cellular processes. The function of the Rho2 and Rho5 proteins from Saccharomyces cerevisiae and of their homologs in other species is poorly understood. Here, we report on the analysis of the AgRho2 and AgRho5 proteins of the filamentous fungus Ashbya gossypii. In contrast to S. cerevisiae mutants of both encoding genes displayed a strong morphological phenotype. The Agrho2 mutants showed defects in tip-branching, while Agrho5 mutants had a significantly decreased growth rate and failed to maintain their growth axis. In addition, the Agrho5 mutants had highly defective actin rings at septation sites. We also found that a deletion mutant of a putative GDP-GTP-exchange factor (GEF) that was homologous to a Rac-GEF from other species phenocopied the Agrho5 mutant, suggesting that both proteins act in the same pathway, but the AgRho5 protein has acquired functions that are fulfilled by Rac-proteins in other species.
format	article
author	Doris Nordmann Manuela Lickfeld Verena Warnsmann Johanna Wiechert Arne Jendretzki Hans-Peter Schmitz
author_facet	Doris Nordmann Manuela Lickfeld Verena Warnsmann Johanna Wiechert Arne Jendretzki Hans-Peter Schmitz
author_sort	Doris Nordmann
title	The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.
title_short	The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.
title_full	The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.
title_fullStr	The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.
title_full_unstemmed	The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.
title_sort	small gtp-binding proteins agrho2 and agrho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus ashbya gossypii.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/800c0a527a204196a1ad4e3b4f4e0535
work_keys_str_mv	AT dorisnordmann thesmallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT manuelalickfeld thesmallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT verenawarnsmann thesmallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT johannawiechert thesmallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT arnejendretzki thesmallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT hanspeterschmitz thesmallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT dorisnordmann smallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT manuelalickfeld smallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT verenawarnsmann smallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT johannawiechert smallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT arnejendretzki smallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii AT hanspeterschmitz smallgtpbindingproteinsagrho2andagrho5regulatetipbranchingmaintenanceofthegrowthaxisandactinringintegrityinthefilamentousfungusashbyagossypii
_version_	1718414231751098368

The small GTP-binding proteins AgRho2 and AgRho5 regulate tip-branching, maintenance of the growth axis and actin-ring-integrity in the filamentous fungus Ashbya gossypii.

Ejemplares similares