The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis

IgA nephropathy is the most common glomerular disease in the world and has become a serious threat to human health. Accurate and non-invasive molecular imaging to detect and recognize the IgA nephropathy is critical for the subsequent timely treatment; otherwise, it may progress to end-stage renal d...

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Autores principales:	Yaoyao Wu, Qiang Huang, Junli Wang, Yuhua Dai, Ming Xiao, Yangyang Li, Hongbo Zhang, Wenbo Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	IgA nephropathy (IgAN) Fe3O4-RGD αvβ3-targeted noninvasively diagnosis T2 weighted MR imaging Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/801c823dc20748a59e0d8caed4273d84
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spelling	oai:doaj.org-article:801c823dc20748a59e0d8caed4273d842021-12-01T01:36:05ZThe Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis2296-418510.3389/fbioe.2021.755692https://doaj.org/article/801c823dc20748a59e0d8caed4273d842021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.755692/fullhttps://doaj.org/toc/2296-4185IgA nephropathy is the most common glomerular disease in the world and has become a serious threat to human health. Accurate and non-invasive molecular imaging to detect and recognize the IgA nephropathy is critical for the subsequent timely treatment; otherwise, it may progress to end-stage renal disease and lead to glomerular dysfunction. In this study, we have developed a sensitive, specific, and biocompatible integrin αvβ3-targeted superparamagnetic Fe3O4 nanoparticles (NPs) for the noninvasive magnetic resonance imaging (MRI) of integrin αvβ3, which is overexpressed in glomerular mesangial region of IgA nephropathy. The rat model of IgA nephropathy was successfully established and verified by biochemical tests and histological staining. Meanwhile, the clinical 18F-AlF-NOTA-PRGD2 probe molecule was utilized to visualize and further confirmed the IgA nephropathy in vivo via positron emission computed tomography. Subsequently, the Fe3O4 NPs were conjugated with arginine–glycine–aspartic acid (RGD) molecules (Fe3O4-RGD), and their integrin αvβ3-targeted T2-weighted imaging (T2WI) potential has been carefully evaluated. The Fe3O4-RGD demonstrated great relaxation in vivo. The T2WI signal of renal layers in the targeted group at 3 h after intravenous injection of Fe3O4-RGD was distinctly lower than baseline, indicating MRI signal decreased in the established IgA nephropathy rat model. Moreover, the TEM characterization and Prussian blue staining confirmed that the Fe3O4-RGD was located at the region of glomerulus and tubular interstitium. Moreover, no obvious signal decreased was detected in the untargeted Fe3O4 treated and normal groups. Collectively, our results establish the possibility of Fe3O4-RGD serving as a feasible MRI agent for the noninvasive diagnosis of IgA nephropathy.Yaoyao WuQiang HuangJunli WangYuhua DaiMing XiaoYangyang LiHongbo ZhangWenbo XiaoFrontiers Media S.A.articleIgA nephropathy (IgAN)Fe3O4-RGDαvβ3-targetednoninvasively diagnosisT2 weighted MR imagingBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	IgA nephropathy (IgAN) Fe3O4-RGD αvβ3-targeted noninvasively diagnosis T2 weighted MR imaging Biotechnology TP248.13-248.65
spellingShingle	IgA nephropathy (IgAN) Fe3O4-RGD αvβ3-targeted noninvasively diagnosis T2 weighted MR imaging Biotechnology TP248.13-248.65 Yaoyao Wu Qiang Huang Junli Wang Yuhua Dai Ming Xiao Yangyang Li Hongbo Zhang Wenbo Xiao The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis
description	IgA nephropathy is the most common glomerular disease in the world and has become a serious threat to human health. Accurate and non-invasive molecular imaging to detect and recognize the IgA nephropathy is critical for the subsequent timely treatment; otherwise, it may progress to end-stage renal disease and lead to glomerular dysfunction. In this study, we have developed a sensitive, specific, and biocompatible integrin αvβ3-targeted superparamagnetic Fe3O4 nanoparticles (NPs) for the noninvasive magnetic resonance imaging (MRI) of integrin αvβ3, which is overexpressed in glomerular mesangial region of IgA nephropathy. The rat model of IgA nephropathy was successfully established and verified by biochemical tests and histological staining. Meanwhile, the clinical 18F-AlF-NOTA-PRGD2 probe molecule was utilized to visualize and further confirmed the IgA nephropathy in vivo via positron emission computed tomography. Subsequently, the Fe3O4 NPs were conjugated with arginine–glycine–aspartic acid (RGD) molecules (Fe3O4-RGD), and their integrin αvβ3-targeted T2-weighted imaging (T2WI) potential has been carefully evaluated. The Fe3O4-RGD demonstrated great relaxation in vivo. The T2WI signal of renal layers in the targeted group at 3 h after intravenous injection of Fe3O4-RGD was distinctly lower than baseline, indicating MRI signal decreased in the established IgA nephropathy rat model. Moreover, the TEM characterization and Prussian blue staining confirmed that the Fe3O4-RGD was located at the region of glomerulus and tubular interstitium. Moreover, no obvious signal decreased was detected in the untargeted Fe3O4 treated and normal groups. Collectively, our results establish the possibility of Fe3O4-RGD serving as a feasible MRI agent for the noninvasive diagnosis of IgA nephropathy.
format	article
author	Yaoyao Wu Qiang Huang Junli Wang Yuhua Dai Ming Xiao Yangyang Li Hongbo Zhang Wenbo Xiao
author_facet	Yaoyao Wu Qiang Huang Junli Wang Yuhua Dai Ming Xiao Yangyang Li Hongbo Zhang Wenbo Xiao
author_sort	Yaoyao Wu
title	The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis
title_short	The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis
title_full	The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis
title_fullStr	The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis
title_full_unstemmed	The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis
title_sort	feasibility of targeted magnetic iron oxide nanoagent for noninvasive iga nephropathy diagnosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/801c823dc20748a59e0d8caed4273d84
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The Feasibility of Targeted Magnetic Iron Oxide Nanoagent for Noninvasive IgA Nephropathy Diagnosis

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