Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen

Abstract Recent studies have highlighted great challenges of transgene silencing for transgenic plants facing climate change. In order to understand the impacts of elevated CO2 on exogenous Bacillus thuringiensis (Bt) toxins and transgene expression in transgenic rice under different levels of N-fer...

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Autores principales:	Shoulin Jiang, Yongqing Lu, Yang Dai, Lei Qian, Adnan Bodlah Muhammad, Teng Li, Guijun Wan, Megha N. Parajulee, Fajun Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8023c54999104929b8f56deb9a0356ec
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spelling	oai:doaj.org-article:8023c54999104929b8f56deb9a0356ec2021-12-02T15:05:42ZImpacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen10.1038/s41598-017-15321-92045-2322https://doaj.org/article/8023c54999104929b8f56deb9a0356ec2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15321-9https://doaj.org/toc/2045-2322Abstract Recent studies have highlighted great challenges of transgene silencing for transgenic plants facing climate change. In order to understand the impacts of elevated CO2 on exogenous Bacillus thuringiensis (Bt) toxins and transgene expression in transgenic rice under different levels of N-fertilizer supply, we investigated the biomass, exogenous Bt toxins, Bt-transgene expression and methylation status in Bt rice exposed to two levels of CO2 concentrations and nitrogen (N) supply (1/8, 1/4, 1/2, 1 and 2 N). It is elucidated that the increased levels of global atmospheric CO2 concentration will trigger up-regulation of Bt toxin expression in transgenic rice, especially with appropriate increase of N fertilizer supply, while, to some extent, the exogenous Bt-transgene expression is reduced at sub-N levels (1/4 and 1/2N), even though the total protein of plant tissues is reduced and the plant growth is restricted. The unpredictable and stochastic occurrence of transgene silencing and epigenetic alternations remains unresolved for most transgenic plants. It is expected that N fertilization supply may promote the expression of transgenic Bt toxin in transgenic Bt rice, particularly under elevated CO2.Shoulin JiangYongqing LuYang DaiLei QianAdnan Bodlah MuhammadTeng LiGuijun WanMegha N. ParajuleeFajun ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shoulin Jiang Yongqing Lu Yang Dai Lei Qian Adnan Bodlah Muhammad Teng Li Guijun Wan Megha N. Parajulee Fajun Chen Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen
description	Abstract Recent studies have highlighted great challenges of transgene silencing for transgenic plants facing climate change. In order to understand the impacts of elevated CO2 on exogenous Bacillus thuringiensis (Bt) toxins and transgene expression in transgenic rice under different levels of N-fertilizer supply, we investigated the biomass, exogenous Bt toxins, Bt-transgene expression and methylation status in Bt rice exposed to two levels of CO2 concentrations and nitrogen (N) supply (1/8, 1/4, 1/2, 1 and 2 N). It is elucidated that the increased levels of global atmospheric CO2 concentration will trigger up-regulation of Bt toxin expression in transgenic rice, especially with appropriate increase of N fertilizer supply, while, to some extent, the exogenous Bt-transgene expression is reduced at sub-N levels (1/4 and 1/2N), even though the total protein of plant tissues is reduced and the plant growth is restricted. The unpredictable and stochastic occurrence of transgene silencing and epigenetic alternations remains unresolved for most transgenic plants. It is expected that N fertilization supply may promote the expression of transgenic Bt toxin in transgenic Bt rice, particularly under elevated CO2.
format	article
author	Shoulin Jiang Yongqing Lu Yang Dai Lei Qian Adnan Bodlah Muhammad Teng Li Guijun Wan Megha N. Parajulee Fajun Chen
author_facet	Shoulin Jiang Yongqing Lu Yang Dai Lei Qian Adnan Bodlah Muhammad Teng Li Guijun Wan Megha N. Parajulee Fajun Chen
author_sort	Shoulin Jiang
title	Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen
title_short	Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen
title_full	Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen
title_fullStr	Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen
title_full_unstemmed	Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen
title_sort	impacts of elevated co2 on exogenous bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8023c54999104929b8f56deb9a0356ec
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Impacts of elevated CO2 on exogenous Bacillus thuringiensis toxins and transgene expression in transgenic rice under different levels of nitrogen

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