Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing

Abstract Corneal wound healing depends on extracellular matrix (ECM) and topographical cues that modulate migration and proliferation of regenerating cells. In our study, silk films with either flat or nanotopography patterned parallel ridge widths of 2000, 1000, 800 nm surfaces were combined with E...

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Autores principales:	Yuncin Luo, Kai B. Kang, Rachel Sartaj, Michael G. Sun, Qiang Zhou, Victor H. Guaiquil, Mark I. Rosenblatt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8028378e37924883a8098fa07dfd293a
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spelling	oai:doaj.org-article:8028378e37924883a8098fa07dfd293a2021-12-02T14:26:25ZSilk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing10.1038/s41598-021-87658-12045-2322https://doaj.org/article/8028378e37924883a8098fa07dfd293a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87658-1https://doaj.org/toc/2045-2322Abstract Corneal wound healing depends on extracellular matrix (ECM) and topographical cues that modulate migration and proliferation of regenerating cells. In our study, silk films with either flat or nanotopography patterned parallel ridge widths of 2000, 1000, 800 nm surfaces were combined with ECMs which include collagen type I (collagen I), fibronectin, laminin, and Poly-d-Lysine to accelerate corneal wound healing. Silk films with 800 nm ridge width provided better cell spreading and wound recovery than other size topographies. Coating 800 nm patterned silk films with collagen I proves to optimally further increased mouse and rabbit corneal epithelial cells growth and wound recovery. This enhanced cellular response correlated with redistribution and increase in size and total amount of focal adhesion. Transcriptomics and signaling pathway analysis suggested that silk topography regulates cell behaviors via actin nucleation ARP-WASP complex pathway, which regulate filopodia formation. This mechanism was further explored and inhibition of Cdc42, a key protein in this pathway, delayed wound healing and decreased the length, density, and alignment of filopodia. Inhibition of Cdc42 in vivo resulted in delayed re-epithelization of injured corneas. We conclude that silk film nanotopography in combination with collagen I constitutes a better substrate for corneal wound repair than either nanotopography or ECM alone.Yuncin LuoKai B. KangRachel SartajMichael G. SunQiang ZhouVictor H. GuaiquilMark I. RosenblattNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuncin Luo Kai B. Kang Rachel Sartaj Michael G. Sun Qiang Zhou Victor H. Guaiquil Mark I. Rosenblatt Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing
description	Abstract Corneal wound healing depends on extracellular matrix (ECM) and topographical cues that modulate migration and proliferation of regenerating cells. In our study, silk films with either flat or nanotopography patterned parallel ridge widths of 2000, 1000, 800 nm surfaces were combined with ECMs which include collagen type I (collagen I), fibronectin, laminin, and Poly-d-Lysine to accelerate corneal wound healing. Silk films with 800 nm ridge width provided better cell spreading and wound recovery than other size topographies. Coating 800 nm patterned silk films with collagen I proves to optimally further increased mouse and rabbit corneal epithelial cells growth and wound recovery. This enhanced cellular response correlated with redistribution and increase in size and total amount of focal adhesion. Transcriptomics and signaling pathway analysis suggested that silk topography regulates cell behaviors via actin nucleation ARP-WASP complex pathway, which regulate filopodia formation. This mechanism was further explored and inhibition of Cdc42, a key protein in this pathway, delayed wound healing and decreased the length, density, and alignment of filopodia. Inhibition of Cdc42 in vivo resulted in delayed re-epithelization of injured corneas. We conclude that silk film nanotopography in combination with collagen I constitutes a better substrate for corneal wound repair than either nanotopography or ECM alone.
format	article
author	Yuncin Luo Kai B. Kang Rachel Sartaj Michael G. Sun Qiang Zhou Victor H. Guaiquil Mark I. Rosenblatt
author_facet	Yuncin Luo Kai B. Kang Rachel Sartaj Michael G. Sun Qiang Zhou Victor H. Guaiquil Mark I. Rosenblatt
author_sort	Yuncin Luo
title	Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing
title_short	Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing
title_full	Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing
title_fullStr	Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing
title_full_unstemmed	Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing
title_sort	silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8028378e37924883a8098fa07dfd293a
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Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing

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