Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus

Abstract. Raharja NI, Widanarni, Wahyudi AT. 2019. Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus. Biodiversitas 20: 3164-3169. Sponge-associated bacteria are commonly known as an excellent source of microbial's bioactive...

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Autores principales:	NEPTU ISLAMY RAHARJA, WIDANARNI, Aris Tri Wahyudi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	16s-rrna, bioactive compounds, gc-ms, sponge-associated bacteria, vibrio parahaemolyticus, whiteleg shrimp Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/802f34d5178647a0a792ef192858a52e
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spelling	oai:doaj.org-article:802f34d5178647a0a792ef192858a52e2021-11-21T21:57:24ZMarine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus1412-033X2085-472210.13057/biodiv/d201108https://doaj.org/article/802f34d5178647a0a792ef192858a52e2019-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4370https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Raharja NI, Widanarni, Wahyudi AT. 2019. Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus. Biodiversitas 20: 3164-3169. Sponge-associated bacteria are commonly known as an excellent source of microbial's bioactive compounds attributed to biological properties, including antibacterial activity. In our previous study, 12 isolates from sponge Aaptos sp. and Hyrtios have been screened to have anti-Vibrio spp. activities in vitro. The objective of this study was to evaluate these bacterial isolates for controlling Vibrio parahaemolyticus infection in vivo, identify the most potential isolate, and analyze chemical composition of bacterial crude extract. As tested by pathogenicity assay on post-larvae of whiteleg shrimp stadia PL 10, all 12 isolates were not pathogenic bacteria as indicated by the survival rate of shrimps ranging from 80 ± 8.1 - 93 ± 4.7%. Conversely, more than 50% died after inoculated with pathogenic V. parahaemolyticus. Interestingly, all isolates could increase the survival rate of shrimps infected with V. parahaemolyticus (105 cells/mL) reach of 80 ± 0.8 - 91 ± 0.4%, nearly 40% higher than the positive control, as evaluated by challenge test. The best anti-Vibrio activity showed by D2.Z13 isolate which could improve the survival rate up to 91.11 ± 0.4%. 16S-rRNA-based identification showed that this isolate was closely related to Pseudomonas aeruginosa 1-4-b-9 strain. The extract derived from this isolate contained some major compounds such as 4,8-dihydroxy-2-2 (1’-hydroxyheptyl) and 1,2,4-oxadiazol which have been reported as antibacterial compounds. Our study indicates that these bacterial isolates could be developed as biocontrol agents of vibriosis in whiteleg shrimp.NEPTU ISLAMY RAHARJAWIDANARNIAris Tri WahyudiMBI & UNS Soloarticle16s-rrna, bioactive compounds, gc-ms, sponge-associated bacteria, vibrio parahaemolyticus, whiteleg shrimpBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s-rrna, bioactive compounds, gc-ms, sponge-associated bacteria, vibrio parahaemolyticus, whiteleg shrimp Biology (General) QH301-705.5
spellingShingle	16s-rrna, bioactive compounds, gc-ms, sponge-associated bacteria, vibrio parahaemolyticus, whiteleg shrimp Biology (General) QH301-705.5 NEPTU ISLAMY RAHARJA WIDANARNI Aris Tri Wahyudi Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus
description	Abstract. Raharja NI, Widanarni, Wahyudi AT. 2019. Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus. Biodiversitas 20: 3164-3169. Sponge-associated bacteria are commonly known as an excellent source of microbial's bioactive compounds attributed to biological properties, including antibacterial activity. In our previous study, 12 isolates from sponge Aaptos sp. and Hyrtios have been screened to have anti-Vibrio spp. activities in vitro. The objective of this study was to evaluate these bacterial isolates for controlling Vibrio parahaemolyticus infection in vivo, identify the most potential isolate, and analyze chemical composition of bacterial crude extract. As tested by pathogenicity assay on post-larvae of whiteleg shrimp stadia PL 10, all 12 isolates were not pathogenic bacteria as indicated by the survival rate of shrimps ranging from 80 ± 8.1 - 93 ± 4.7%. Conversely, more than 50% died after inoculated with pathogenic V. parahaemolyticus. Interestingly, all isolates could increase the survival rate of shrimps infected with V. parahaemolyticus (105 cells/mL) reach of 80 ± 0.8 - 91 ± 0.4%, nearly 40% higher than the positive control, as evaluated by challenge test. The best anti-Vibrio activity showed by D2.Z13 isolate which could improve the survival rate up to 91.11 ± 0.4%. 16S-rRNA-based identification showed that this isolate was closely related to Pseudomonas aeruginosa 1-4-b-9 strain. The extract derived from this isolate contained some major compounds such as 4,8-dihydroxy-2-2 (1’-hydroxyheptyl) and 1,2,4-oxadiazol which have been reported as antibacterial compounds. Our study indicates that these bacterial isolates could be developed as biocontrol agents of vibriosis in whiteleg shrimp.
format	article
author	NEPTU ISLAMY RAHARJA WIDANARNI Aris Tri Wahyudi
author_facet	NEPTU ISLAMY RAHARJA WIDANARNI Aris Tri Wahyudi
author_sort	NEPTU ISLAMY RAHARJA
title	Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus
title_short	Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus
title_full	Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus
title_fullStr	Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus
title_full_unstemmed	Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus
title_sort	marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by vibrio parahaemolyticus
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/802f34d5178647a0a792ef192858a52e
work_keys_str_mv	AT neptuislamyraharja marinespongeassociatedbacteriaasbiocontrolagentsofvibriosisonwhitelegshrimpcausedbyvibrioparahaemolyticus AT widanarni marinespongeassociatedbacteriaasbiocontrolagentsofvibriosisonwhitelegshrimpcausedbyvibrioparahaemolyticus AT aristriwahyudi marinespongeassociatedbacteriaasbiocontrolagentsofvibriosisonwhitelegshrimpcausedbyvibrioparahaemolyticus
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Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus

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