Centromere size scales with genome size across Eukaryotes

Abstract Previous studies on grass species suggested that the total centromere size (sum of all centromere sizes in a cell) may be determined by the genome size, possibly because stable scaling is important for proper cell division. However, it is unclear whether this relationship is universal. Here...

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Autores principales:	Klára Plačková, Petr Bureš, František Zedek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80309d4c0e9e41d4aab06e225212d91d
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spelling	oai:doaj.org-article:80309d4c0e9e41d4aab06e225212d91d2021-12-02T19:16:10ZCentromere size scales with genome size across Eukaryotes10.1038/s41598-021-99386-72045-2322https://doaj.org/article/80309d4c0e9e41d4aab06e225212d91d2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99386-7https://doaj.org/toc/2045-2322Abstract Previous studies on grass species suggested that the total centromere size (sum of all centromere sizes in a cell) may be determined by the genome size, possibly because stable scaling is important for proper cell division. However, it is unclear whether this relationship is universal. Here we analyze the total centromere size using the CenH3-immunofluorescence area as a proxy in 130 taxa including plants, animals, fungi, and protists. We verified the reliability of our methodological approach by comparing our measurements with available ChIP-seq-based measurements of the size of CenH3-binding domains. Data based on these two independent methods showed the same positive relationship between the total centromere size and genome size. Our results demonstrate that the genome size is a strong predictor (R-squared = 0.964) of the total centromere size universally across Eukaryotes. We also show that this relationship is independent of phylogenetic relatedness and centromere type (monocentric, metapolycentric, and holocentric), implying a common mechanism maintaining stable total centromere size in Eukaryotes.Klára PlačkováPetr BurešFrantišek ZedekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Klára Plačková Petr Bureš František Zedek Centromere size scales with genome size across Eukaryotes
description	Abstract Previous studies on grass species suggested that the total centromere size (sum of all centromere sizes in a cell) may be determined by the genome size, possibly because stable scaling is important for proper cell division. However, it is unclear whether this relationship is universal. Here we analyze the total centromere size using the CenH3-immunofluorescence area as a proxy in 130 taxa including plants, animals, fungi, and protists. We verified the reliability of our methodological approach by comparing our measurements with available ChIP-seq-based measurements of the size of CenH3-binding domains. Data based on these two independent methods showed the same positive relationship between the total centromere size and genome size. Our results demonstrate that the genome size is a strong predictor (R-squared = 0.964) of the total centromere size universally across Eukaryotes. We also show that this relationship is independent of phylogenetic relatedness and centromere type (monocentric, metapolycentric, and holocentric), implying a common mechanism maintaining stable total centromere size in Eukaryotes.
format	article
author	Klára Plačková Petr Bureš František Zedek
author_facet	Klára Plačková Petr Bureš František Zedek
author_sort	Klára Plačková
title	Centromere size scales with genome size across Eukaryotes
title_short	Centromere size scales with genome size across Eukaryotes
title_full	Centromere size scales with genome size across Eukaryotes
title_fullStr	Centromere size scales with genome size across Eukaryotes
title_full_unstemmed	Centromere size scales with genome size across Eukaryotes
title_sort	centromere size scales with genome size across eukaryotes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/80309d4c0e9e41d4aab06e225212d91d
work_keys_str_mv	AT klaraplackova centromeresizescaleswithgenomesizeacrosseukaryotes AT petrbures centromeresizescaleswithgenomesizeacrosseukaryotes AT frantisekzedek centromeresizescaleswithgenomesizeacrosseukaryotes
_version_	1718376979557777408

Centromere size scales with genome size across Eukaryotes

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