Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer

Wnt/β‐catenin signaling is an evolutionarily conserved pathway and plays a crucial role in regulating cancer cell proliferation and tumorigenesis. However, the molecular mechanism behind the Wnt/β‐catenin signaling-mediated carcinogenesis and apoptosis resistance in oral squamous cell carcinoma is n...

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Autores principales:	Wang Yufeng, Cao Zheng, Liu Fengjia, Ou Yuejian
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	apoptosis β-catenin cell proliferation oral cancer tumorigenesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/803aa05acdef4096bc2376c9b2cec12b
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spelling	oai:doaj.org-article:803aa05acdef4096bc2376c9b2cec12b2021-12-05T14:10:41ZClinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer2391-541210.1515/biol-2021-0104https://doaj.org/article/803aa05acdef4096bc2376c9b2cec12b2021-09-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0104https://doaj.org/toc/2391-5412Wnt/β‐catenin signaling is an evolutionarily conserved pathway and plays a crucial role in regulating cancer cell proliferation and tumorigenesis. However, the molecular mechanism behind the Wnt/β‐catenin signaling-mediated carcinogenesis and apoptosis resistance in oral squamous cell carcinoma is not well characterized so far. In the present study, we have investigated the effect of β‐catenin depletion of the perversely activated Wnt/β-catenin signaling pathway on apoptosis resistance and tumorigenesis of the human OSCC cell line SCC-55. RT-PCR and western blot analysis demonstrated that the Wnt/β-catenin signaling pathway and its downstream targets such as DKK1 and AXIN2 are aberrantly activated in SCC-55 cells. Furthermore, upon silencing (RNA interference) of β‐catenin in SCC-55, cells became more sensitive toward the chemotherapeutic drugs and thus resulted in apoptotic cell death. Meanwhile, flow cytometry analysis confirmed the enhanced apoptosis and activation of caspases in β‐catenin RNAi cells. Besides ensuing β-catenin–siRNA transfection, the cell proliferation and cancer colony generating efficiencies are significantly impeded compared to the non-transfected cells. Furthermore, the tumorigenicity was inhibited by the downregulation of OCT-4 in β‐catenin-silenced SCC-55 cells. Altogether, Wnt/β‐catenin signaling could potentially target anti-cancer drugs to induce apoptosis and achieve a better clinical outcome.Wang YufengCao ZhengLiu FengjiaOu YuejianDe Gruyterarticleapoptosisβ-catenincell proliferationoral cancertumorigenesisBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1045-1052 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis β-catenin cell proliferation oral cancer tumorigenesis Biology (General) QH301-705.5
spellingShingle	apoptosis β-catenin cell proliferation oral cancer tumorigenesis Biology (General) QH301-705.5 Wang Yufeng Cao Zheng Liu Fengjia Ou Yuejian Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
description	Wnt/β‐catenin signaling is an evolutionarily conserved pathway and plays a crucial role in regulating cancer cell proliferation and tumorigenesis. However, the molecular mechanism behind the Wnt/β‐catenin signaling-mediated carcinogenesis and apoptosis resistance in oral squamous cell carcinoma is not well characterized so far. In the present study, we have investigated the effect of β‐catenin depletion of the perversely activated Wnt/β-catenin signaling pathway on apoptosis resistance and tumorigenesis of the human OSCC cell line SCC-55. RT-PCR and western blot analysis demonstrated that the Wnt/β-catenin signaling pathway and its downstream targets such as DKK1 and AXIN2 are aberrantly activated in SCC-55 cells. Furthermore, upon silencing (RNA interference) of β‐catenin in SCC-55, cells became more sensitive toward the chemotherapeutic drugs and thus resulted in apoptotic cell death. Meanwhile, flow cytometry analysis confirmed the enhanced apoptosis and activation of caspases in β‐catenin RNAi cells. Besides ensuing β-catenin–siRNA transfection, the cell proliferation and cancer colony generating efficiencies are significantly impeded compared to the non-transfected cells. Furthermore, the tumorigenicity was inhibited by the downregulation of OCT-4 in β‐catenin-silenced SCC-55 cells. Altogether, Wnt/β‐catenin signaling could potentially target anti-cancer drugs to induce apoptosis and achieve a better clinical outcome.
format	article
author	Wang Yufeng Cao Zheng Liu Fengjia Ou Yuejian
author_facet	Wang Yufeng Cao Zheng Liu Fengjia Ou Yuejian
author_sort	Wang Yufeng
title	Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
title_short	Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
title_full	Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
title_fullStr	Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
title_full_unstemmed	Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer
title_sort	clinical significance of activated wnt/β-catenin signaling in apoptosis inhibition of oral cancer
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/803aa05acdef4096bc2376c9b2cec12b
work_keys_str_mv	AT wangyufeng clinicalsignificanceofactivatedwntbcateninsignalinginapoptosisinhibitionoforalcancer AT caozheng clinicalsignificanceofactivatedwntbcateninsignalinginapoptosisinhibitionoforalcancer AT liufengjia clinicalsignificanceofactivatedwntbcateninsignalinginapoptosisinhibitionoforalcancer AT ouyuejian clinicalsignificanceofactivatedwntbcateninsignalinginapoptosisinhibitionoforalcancer
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Clinical significance of activated Wnt/β-catenin signaling in apoptosis inhibition of oral cancer

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