Eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.

Eccentric, or lengthening, contractions result in injury and subsequently stimulate the activation and proliferation of satellite stem cells which are important for skeletal muscle regeneration. The discovery of alternative myogenic progenitors in skeletal muscle raises the question as to whether st...

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Autores principales: M Carmen Valero, Heather D Huntsman, Jianming Liu, Kai Zou, Marni D Boppart
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Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/803bc16326aa42f8ad078cfa1ca5d0df
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spelling oai:doaj.org-article:803bc16326aa42f8ad078cfa1ca5d0df2021-11-18T07:30:29ZEccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.1932-620310.1371/journal.pone.0029760https://doaj.org/article/803bc16326aa42f8ad078cfa1ca5d0df2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253772/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Eccentric, or lengthening, contractions result in injury and subsequently stimulate the activation and proliferation of satellite stem cells which are important for skeletal muscle regeneration. The discovery of alternative myogenic progenitors in skeletal muscle raises the question as to whether stem cells other than satellite cells accumulate in muscle in response to exercise and contribute to post-exercise repair and/or growth. In this study, stem cell antigen-1 (Sca-1) positive, non-hematopoetic (CD45⁻) cells were evaluated in wild type (WT) and α7 integrin transgenic (α7Tg) mouse muscle, which is resistant to injury yet liable to strain, 24 hr following a single bout of eccentric exercise. Sca-1⁺CD45⁻ stem cells were increased 2-fold in WT muscle post-exercise. The α7 integrin regulated the presence of Sca-1⁺ cells, with expansion occurring in α7Tg muscle and minimal cells present in muscle lacking the α7 integrin. Sca-1⁺CD45⁻ cells isolated from α7Tg muscle following exercise were characterized as mesenchymal-like stem cells (mMSCs), predominantly pericytes. In vitro multiaxial strain upregulated mMSC stem cells markers in the presence of laminin, but not gelatin, identifying a potential mechanistic basis for the accumulation of these cells in muscle following exercise. Transplantation of DiI-labeled mMSCs into WT muscle increased Pax7⁺ cells and facilitated formation of eMHC⁺DiI⁻ fibers. This study provides the first demonstration that mMSCs rapidly appear in skeletal muscle in an α7 integrin dependent manner post-exercise, revealing an early event that may be necessary for effective repair and/or growth following exercise. The results from this study also support a role for the α7 integrin and/or mMSCs in molecular- and cellular-based therapeutic strategies that can effectively combat disuse muscle atrophy.M Carmen ValeroHeather D HuntsmanJianming LiuKai ZouMarni D BoppartPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29760 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
M Carmen Valero
Heather D Huntsman
Jianming Liu
Kai Zou
Marni D Boppart
Eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.
description Eccentric, or lengthening, contractions result in injury and subsequently stimulate the activation and proliferation of satellite stem cells which are important for skeletal muscle regeneration. The discovery of alternative myogenic progenitors in skeletal muscle raises the question as to whether stem cells other than satellite cells accumulate in muscle in response to exercise and contribute to post-exercise repair and/or growth. In this study, stem cell antigen-1 (Sca-1) positive, non-hematopoetic (CD45⁻) cells were evaluated in wild type (WT) and α7 integrin transgenic (α7Tg) mouse muscle, which is resistant to injury yet liable to strain, 24 hr following a single bout of eccentric exercise. Sca-1⁺CD45⁻ stem cells were increased 2-fold in WT muscle post-exercise. The α7 integrin regulated the presence of Sca-1⁺ cells, with expansion occurring in α7Tg muscle and minimal cells present in muscle lacking the α7 integrin. Sca-1⁺CD45⁻ cells isolated from α7Tg muscle following exercise were characterized as mesenchymal-like stem cells (mMSCs), predominantly pericytes. In vitro multiaxial strain upregulated mMSC stem cells markers in the presence of laminin, but not gelatin, identifying a potential mechanistic basis for the accumulation of these cells in muscle following exercise. Transplantation of DiI-labeled mMSCs into WT muscle increased Pax7⁺ cells and facilitated formation of eMHC⁺DiI⁻ fibers. This study provides the first demonstration that mMSCs rapidly appear in skeletal muscle in an α7 integrin dependent manner post-exercise, revealing an early event that may be necessary for effective repair and/or growth following exercise. The results from this study also support a role for the α7 integrin and/or mMSCs in molecular- and cellular-based therapeutic strategies that can effectively combat disuse muscle atrophy.
format article
author M Carmen Valero
Heather D Huntsman
Jianming Liu
Kai Zou
Marni D Boppart
author_facet M Carmen Valero
Heather D Huntsman
Jianming Liu
Kai Zou
Marni D Boppart
author_sort M Carmen Valero
title Eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.
title_short Eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.
title_full Eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.
title_fullStr Eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.
title_full_unstemmed Eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.
title_sort eccentric exercise facilitates mesenchymal stem cell appearance in skeletal muscle.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/803bc16326aa42f8ad078cfa1ca5d0df
work_keys_str_mv AT mcarmenvalero eccentricexercisefacilitatesmesenchymalstemcellappearanceinskeletalmuscle
AT heatherdhuntsman eccentricexercisefacilitatesmesenchymalstemcellappearanceinskeletalmuscle
AT jianmingliu eccentricexercisefacilitatesmesenchymalstemcellappearanceinskeletalmuscle
AT kaizou eccentricexercisefacilitatesmesenchymalstemcellappearanceinskeletalmuscle
AT marnidboppart eccentricexercisefacilitatesmesenchymalstemcellappearanceinskeletalmuscle
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