High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells

Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.

Guardado en:
Detalles Bibliográficos
Autores principales: Arndt Steube, Tino Schenk, Alexander Tretyakov, Hans Peter Saluz
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Q
Acceso en línea:https://doaj.org/article/8044421ab89d460e975d3817b5e10749
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
Descripción
Sumario:Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.