High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells

Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.

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Autores principales:	Arndt Steube, Tino Schenk, Alexander Tretyakov, Hans Peter Saluz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8044421ab89d460e975d3817b5e10749
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spelling	oai:doaj.org-article:8044421ab89d460e975d3817b5e107492021-12-02T14:42:25ZHigh-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells10.1038/s41467-017-01251-72041-1723https://doaj.org/article/8044421ab89d460e975d3817b5e107492017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01251-7https://doaj.org/toc/2041-1723Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.Arndt SteubeTino SchenkAlexander TretyakovHans Peter SaluzNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Arndt Steube Tino Schenk Alexander Tretyakov Hans Peter Saluz High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
description	Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.
format	article
author	Arndt Steube Tino Schenk Alexander Tretyakov Hans Peter Saluz
author_facet	Arndt Steube Tino Schenk Alexander Tretyakov Hans Peter Saluz
author_sort	Arndt Steube
title	High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
title_short	High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
title_full	High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
title_fullStr	High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
title_full_unstemmed	High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
title_sort	high-intensity uv laser chip-seq for the study of protein-dna interactions in living cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8044421ab89d460e975d3817b5e10749
work_keys_str_mv	AT arndtsteube highintensityuvlaserchipseqforthestudyofproteindnainteractionsinlivingcells AT tinoschenk highintensityuvlaserchipseqforthestudyofproteindnainteractionsinlivingcells AT alexandertretyakov highintensityuvlaserchipseqforthestudyofproteindnainteractionsinlivingcells AT hanspetersaluz highintensityuvlaserchipseqforthestudyofproteindnainteractionsinlivingcells
_version_	1718389697337622528

High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells

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