Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis

Abstract γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid that has hypotensive effects. Tomato (Solanum lycopersicum L.) is among the most widely cultivated and consumed vegetables in the world and contains higher levels of GABA than other major crops. Increasing these levels can further...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Satoko Nonaka, Chikako Arai, Mariko Takayama, Chiaki Matsukura, Hiroshi Ezura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8045483563c54d27942337ddcc936d70
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8045483563c54d27942337ddcc936d70
record_format	dspace
spelling	oai:doaj.org-article:8045483563c54d27942337ddcc936d702021-12-02T16:06:42ZEfficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis10.1038/s41598-017-06400-y2045-2322https://doaj.org/article/8045483563c54d27942337ddcc936d702017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06400-yhttps://doaj.org/toc/2045-2322Abstract γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid that has hypotensive effects. Tomato (Solanum lycopersicum L.) is among the most widely cultivated and consumed vegetables in the world and contains higher levels of GABA than other major crops. Increasing these levels can further enhance the blood pressure-lowering function of tomato fruit. Glutamate decarboxylase (GAD) is a key enzyme in GABA biosynthesis; it has a C-terminal autoinhibitory domain that regulates enzymatic function, and deleting this domain increases GAD activity. The tomato genome has five GAD genes (SlGAD1–5), of which two (SlGAD2 and SlGAD3) are expressed during tomato fruit development. To increase GABA content in tomato, we deleted the autoinhibitory domain of SlGAD2 and SlGAD3 using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)9 technology. Introducing a stop codon immediately before the autoinhibitory domain increased GABA accumulation by 7 to 15 fold while having variable effects on plant and fruit size and yield. This is the first study describing the application of the CRISPR/Cas9 system to increase GABA content in tomato fruits. Our findings provide a basis for the improvement of other types of crop by CRISPR/Cas9-based genetic modification.Satoko NonakaChikako AraiMariko TakayamaChiaki MatsukuraHiroshi EzuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoko Nonaka Chikako Arai Mariko Takayama Chiaki Matsukura Hiroshi Ezura Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis
description	Abstract γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid that has hypotensive effects. Tomato (Solanum lycopersicum L.) is among the most widely cultivated and consumed vegetables in the world and contains higher levels of GABA than other major crops. Increasing these levels can further enhance the blood pressure-lowering function of tomato fruit. Glutamate decarboxylase (GAD) is a key enzyme in GABA biosynthesis; it has a C-terminal autoinhibitory domain that regulates enzymatic function, and deleting this domain increases GAD activity. The tomato genome has five GAD genes (SlGAD1–5), of which two (SlGAD2 and SlGAD3) are expressed during tomato fruit development. To increase GABA content in tomato, we deleted the autoinhibitory domain of SlGAD2 and SlGAD3 using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)9 technology. Introducing a stop codon immediately before the autoinhibitory domain increased GABA accumulation by 7 to 15 fold while having variable effects on plant and fruit size and yield. This is the first study describing the application of the CRISPR/Cas9 system to increase GABA content in tomato fruits. Our findings provide a basis for the improvement of other types of crop by CRISPR/Cas9-based genetic modification.
format	article
author	Satoko Nonaka Chikako Arai Mariko Takayama Chiaki Matsukura Hiroshi Ezura
author_facet	Satoko Nonaka Chikako Arai Mariko Takayama Chiaki Matsukura Hiroshi Ezura
author_sort	Satoko Nonaka
title	Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis
title_short	Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis
title_full	Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis
title_fullStr	Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis
title_full_unstemmed	Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis
title_sort	efficient increase of ɣ-aminobutyric acid (gaba) content in tomato fruits by targeted mutagenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8045483563c54d27942337ddcc936d70
work_keys_str_mv	AT satokononaka efficientincreaseofɣaminobutyricacidgabacontentintomatofruitsbytargetedmutagenesis AT chikakoarai efficientincreaseofɣaminobutyricacidgabacontentintomatofruitsbytargetedmutagenesis AT marikotakayama efficientincreaseofɣaminobutyricacidgabacontentintomatofruitsbytargetedmutagenesis AT chiakimatsukura efficientincreaseofɣaminobutyricacidgabacontentintomatofruitsbytargetedmutagenesis AT hiroshiezura efficientincreaseofɣaminobutyricacidgabacontentintomatofruitsbytargetedmutagenesis
_version_	1718384910493810688

Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis

Ejemplares similares