Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy

Jianfeng Wang,1 Shutao Gao,2 Shuyu Wang,3 Zhaonan Xu,3 Limin Wei31Department of Orthodontics, 2Department of Nuclear Medicine, Handan Central Hospital, Handan, China; 3Department of Pediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China...

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Autores principales:	Wang J, Gao S, Wang S, Xu Z, Wei L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Zinc oxide nanoparticles Mitophagy Tongue cancer Anticancer therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/804ba94e4f734f0088b6c41fbf3881d5
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spelling	oai:doaj.org-article:804ba94e4f734f0088b6c41fbf3881d52021-12-02T08:11:07ZZinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy1178-2013https://doaj.org/article/804ba94e4f734f0088b6c41fbf3881d52018-06-01T00:00:00Zhttps://www.dovepress.com/zinc-oxide-nanoparticles-induce-toxicity-in-cal-27-oral-cancer-cell-li-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jianfeng Wang,1 Shutao Gao,2 Shuyu Wang,3 Zhaonan Xu,3 Limin Wei31Department of Orthodontics, 2Department of Nuclear Medicine, Handan Central Hospital, Handan, China; 3Department of Pediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China Background: Tongue squamous cell carcinoma (tongue cancer) is one of the most common malignancies in the oral maxillofacial region. The tumor easily relapses after surgery, and the prognosis remains poor. Recently, zinc oxide nanoparticles (ZnO NPs) were shown to target multiple cancer cell types. In this study, we aimed to elucidate the anticancer effect of ZnO NPs on CAL 27 human tongue cancer cells and identify the role of PINK1/Parkin-mediated mitophagy in this effect.Materials and methods: We analyzed the dose-dependent cytotoxic effects of ZnO NPs on CAL 27 cells. Cells were cultured in media containing 0, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 µg/mL ZnO NPs for 24 h. We further examined the intracellular reactive oxygen species levels, monodansylcadaverine intensity and mitochondrial membrane potential following the administration of 25 µg/mL ZnO NPs for 4, 8, 12, or 24 h and investigated the role of PINK1/Parkin-mediated mitophagy in ZnO NP-induced toxicity in CAL 27 cells.Results: The viability of CAL 27 cells decreased after treatment with increasing ZnO NP concentrations. The inhibitory concentration 50% of the ZnO NPs was calculated as 25 µg/mL. The ZnO NPs increased the intracellular reactive oxygen species levels and decreased the mitochondrial membrane potential in a time-dependent manner as well as activated the PINK1/Parkin-mediated mitophagy process in CAL 27 cells.Conclusion: Based on our findings, ZnO NPs may possess potential anticancer activity toward tongue cancer cells. Keywords: zinc oxide nanoparticles, mitophagy, tongue cancer; anticancer therapyWang JGao SWang SXu ZWei LDove Medical PressarticleZinc oxide nanoparticlesMitophagyTongue cancerAnticancer therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3441-3450 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zinc oxide nanoparticles Mitophagy Tongue cancer Anticancer therapy Medicine (General) R5-920
spellingShingle	Zinc oxide nanoparticles Mitophagy Tongue cancer Anticancer therapy Medicine (General) R5-920 Wang J Gao S Wang S Xu Z Wei L Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy
description	Jianfeng Wang,1 Shutao Gao,2 Shuyu Wang,3 Zhaonan Xu,3 Limin Wei31Department of Orthodontics, 2Department of Nuclear Medicine, Handan Central Hospital, Handan, China; 3Department of Pediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China Background: Tongue squamous cell carcinoma (tongue cancer) is one of the most common malignancies in the oral maxillofacial region. The tumor easily relapses after surgery, and the prognosis remains poor. Recently, zinc oxide nanoparticles (ZnO NPs) were shown to target multiple cancer cell types. In this study, we aimed to elucidate the anticancer effect of ZnO NPs on CAL 27 human tongue cancer cells and identify the role of PINK1/Parkin-mediated mitophagy in this effect.Materials and methods: We analyzed the dose-dependent cytotoxic effects of ZnO NPs on CAL 27 cells. Cells were cultured in media containing 0, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 µg/mL ZnO NPs for 24 h. We further examined the intracellular reactive oxygen species levels, monodansylcadaverine intensity and mitochondrial membrane potential following the administration of 25 µg/mL ZnO NPs for 4, 8, 12, or 24 h and investigated the role of PINK1/Parkin-mediated mitophagy in ZnO NP-induced toxicity in CAL 27 cells.Results: The viability of CAL 27 cells decreased after treatment with increasing ZnO NP concentrations. The inhibitory concentration 50% of the ZnO NPs was calculated as 25 µg/mL. The ZnO NPs increased the intracellular reactive oxygen species levels and decreased the mitochondrial membrane potential in a time-dependent manner as well as activated the PINK1/Parkin-mediated mitophagy process in CAL 27 cells.Conclusion: Based on our findings, ZnO NPs may possess potential anticancer activity toward tongue cancer cells. Keywords: zinc oxide nanoparticles, mitophagy, tongue cancer; anticancer therapy
format	article
author	Wang J Gao S Wang S Xu Z Wei L
author_facet	Wang J Gao S Wang S Xu Z Wei L
author_sort	Wang J
title	Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy
title_short	Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy
title_full	Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy
title_fullStr	Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy
title_full_unstemmed	Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy
title_sort	zinc oxide nanoparticles induce toxicity in cal 27 oral cancer cell lines by activating pink1/parkin-mediated mitophagy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/804ba94e4f734f0088b6c41fbf3881d5
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Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy

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