Sequencing the connectome.

Connectivity determines the function of neural circuits. Historically, circuit mapping has usually been viewed as a problem of microscopy, but no current method can achieve high-throughput mapping of entire circuits with single neuron precision. Here we describe a novel approach to determining conne...

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Autores principales:	Anthony M Zador, Joshua Dubnau, Hassana K Oyibo, Huiqing Zhan, Gang Cao, Ian D Peikon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8050fee98e15419899cf2ef142b6ae44
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spelling	oai:doaj.org-article:8050fee98e15419899cf2ef142b6ae442021-11-18T05:37:23ZSequencing the connectome.1544-91731545-788510.1371/journal.pbio.1001411https://doaj.org/article/8050fee98e15419899cf2ef142b6ae442012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23109909/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Connectivity determines the function of neural circuits. Historically, circuit mapping has usually been viewed as a problem of microscopy, but no current method can achieve high-throughput mapping of entire circuits with single neuron precision. Here we describe a novel approach to determining connectivity. We propose BOINC ("barcoding of individual neuronal connections"), a method for converting the problem of connectivity into a form that can be read out by high-throughput DNA sequencing. The appeal of using sequencing is that its scale--sequencing billions of nucleotides per day is now routine--is a natural match to the complexity of neural circuits. An inexpensive high-throughput technique for establishing circuit connectivity at single neuron resolution could transform neuroscience research.Anthony M ZadorJoshua DubnauHassana K OyiboHuiqing ZhanGang CaoIan D PeikonPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 10, Iss 10, p e1001411 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Anthony M Zador Joshua Dubnau Hassana K Oyibo Huiqing Zhan Gang Cao Ian D Peikon Sequencing the connectome.
description	Connectivity determines the function of neural circuits. Historically, circuit mapping has usually been viewed as a problem of microscopy, but no current method can achieve high-throughput mapping of entire circuits with single neuron precision. Here we describe a novel approach to determining connectivity. We propose BOINC ("barcoding of individual neuronal connections"), a method for converting the problem of connectivity into a form that can be read out by high-throughput DNA sequencing. The appeal of using sequencing is that its scale--sequencing billions of nucleotides per day is now routine--is a natural match to the complexity of neural circuits. An inexpensive high-throughput technique for establishing circuit connectivity at single neuron resolution could transform neuroscience research.
format	article
author	Anthony M Zador Joshua Dubnau Hassana K Oyibo Huiqing Zhan Gang Cao Ian D Peikon
author_facet	Anthony M Zador Joshua Dubnau Hassana K Oyibo Huiqing Zhan Gang Cao Ian D Peikon
author_sort	Anthony M Zador
title	Sequencing the connectome.
title_short	Sequencing the connectome.
title_full	Sequencing the connectome.
title_fullStr	Sequencing the connectome.
title_full_unstemmed	Sequencing the connectome.
title_sort	sequencing the connectome.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8050fee98e15419899cf2ef142b6ae44
work_keys_str_mv	AT anthonymzador sequencingtheconnectome AT joshuadubnau sequencingtheconnectome AT hassanakoyibo sequencingtheconnectome AT huiqingzhan sequencingtheconnectome AT gangcao sequencingtheconnectome AT iandpeikon sequencingtheconnectome
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Sequencing the connectome.

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