Restriction Enzyme Digestion Exercise – An In-class Activity

Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends in the process of gene cloning, an in-class a...

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Autor principal: Michelle Parent
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2010
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Acceso en línea:https://doaj.org/article/8059b8a0971040b48de198ab55793c9f
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Sumario:Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends in the process of gene cloning, an in-class activity was designed. This restriction enzyme digestion exercise, designed for an introductory undergraduate course in genetics, molecular biology and molecular diagnostics, can be utilized in either a lecture or laboratory setting. Students are provided with information on enzyme discovery and origin, sticky, blunt and compatible ends, base-cutters, isoschizomers and isocaudomers. Students then review the components required for restriction enzyme digestion setup, such as DNA concentration, buffer volume and compatibility and multi-enzyme digestions. Upon completion of the theory review, students participate in this classroom activity where scissors and paper replace restriction enzymes and DNA, providing a visual learning experience.