Restriction Enzyme Digestion Exercise – An In-class Activity

Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends in the process of gene cloning, an in-class a...

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Autor principal:	Michelle Parent
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8059b8a0971040b48de198ab55793c9f
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spelling	oai:doaj.org-article:8059b8a0971040b48de198ab55793c9f2021-11-15T15:04:04ZRestriction Enzyme Digestion Exercise – An In-class Activity10.1128/jmbe.v1.i2.1291935-78851935-7877https://doaj.org/article/8059b8a0971040b48de198ab55793c9f2010-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v1.i2.129https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends in the process of gene cloning, an in-class activity was designed. This restriction enzyme digestion exercise, designed for an introductory undergraduate course in genetics, molecular biology and molecular diagnostics, can be utilized in either a lecture or laboratory setting. Students are provided with information on enzyme discovery and origin, sticky, blunt and compatible ends, base-cutters, isoschizomers and isocaudomers. Students then review the components required for restriction enzyme digestion setup, such as DNA concentration, buffer volume and compatibility and multi-enzyme digestions. Upon completion of the theory review, students participate in this classroom activity where scissors and paper replace restriction enzymes and DNA, providing a visual learning experience.Michelle ParentAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 11, Iss 1, Pp 56-57 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Michelle Parent Restriction Enzyme Digestion Exercise – An In-class Activity
description	Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends in the process of gene cloning, an in-class activity was designed. This restriction enzyme digestion exercise, designed for an introductory undergraduate course in genetics, molecular biology and molecular diagnostics, can be utilized in either a lecture or laboratory setting. Students are provided with information on enzyme discovery and origin, sticky, blunt and compatible ends, base-cutters, isoschizomers and isocaudomers. Students then review the components required for restriction enzyme digestion setup, such as DNA concentration, buffer volume and compatibility and multi-enzyme digestions. Upon completion of the theory review, students participate in this classroom activity where scissors and paper replace restriction enzymes and DNA, providing a visual learning experience.
format	article
author	Michelle Parent
author_facet	Michelle Parent
author_sort	Michelle Parent
title	Restriction Enzyme Digestion Exercise – An In-class Activity
title_short	Restriction Enzyme Digestion Exercise – An In-class Activity
title_full	Restriction Enzyme Digestion Exercise – An In-class Activity
title_fullStr	Restriction Enzyme Digestion Exercise – An In-class Activity
title_full_unstemmed	Restriction Enzyme Digestion Exercise – An In-class Activity
title_sort	restriction enzyme digestion exercise – an in-class activity
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/8059b8a0971040b48de198ab55793c9f
work_keys_str_mv	AT michelleparent restrictionenzymedigestionexerciseaninclassactivity
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Restriction Enzyme Digestion Exercise – An In-class Activity

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