Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.

Land plants are characterised by haplo-diploid life cycles, and developing ovules are the organs in which the haploid and diploid generations coexist. Recently it has been shown that hormones such as auxin and cytokinins play important roles in ovule development and patterning. The establishment and...

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Autores principales:	Luca Ceccato, Simona Masiero, Dola Sinha Roy, Stefano Bencivenga, Irma Roig-Villanova, Franck Anicet Ditengou, Klaus Palme, Rüdiger Simon, Lucia Colombo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/805eb194da7e49c5b9a2b404783e8caa
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spelling	oai:doaj.org-article:805eb194da7e49c5b9a2b404783e8caa2021-11-18T07:41:33ZMaternal control of PIN1 is required for female gametophyte development in Arabidopsis.1932-620310.1371/journal.pone.0066148https://doaj.org/article/805eb194da7e49c5b9a2b404783e8caa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23799075/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Land plants are characterised by haplo-diploid life cycles, and developing ovules are the organs in which the haploid and diploid generations coexist. Recently it has been shown that hormones such as auxin and cytokinins play important roles in ovule development and patterning. The establishment and regulation of auxin levels in cells is predominantly determined by the activity of the auxin efflux carrier proteins PIN-FORMED (PIN). To study the roles of PIN1 and PIN3 during ovule development we have used mutant alleles of both genes and also perturbed PIN1 and PIN3 expression using micro-RNAs controlled by the ovule specific DEFH9 (DEFIFICENS Homologue 9) promoter. PIN1 down-regulation and pin1-5 mutation severely affect female gametophyte development since embryo sacs arrest at the mono- and/or bi-nuclear stages (FG1 and FG3 stage). PIN3 function is not required for ovule development in wild-type or PIN1-silenced plants. We show that sporophytically expressed PIN1 is required for megagametogenesis, suggesting that sporophytic auxin flux might control the early stages of female gametophyte development, although auxin response is not visible in developing embryo sacs.Luca CeccatoSimona MasieroDola Sinha RoyStefano BencivengaIrma Roig-VillanovaFranck Anicet DitengouKlaus PalmeRüdiger SimonLucia ColomboPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66148 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luca Ceccato Simona Masiero Dola Sinha Roy Stefano Bencivenga Irma Roig-Villanova Franck Anicet Ditengou Klaus Palme Rüdiger Simon Lucia Colombo Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.
description	Land plants are characterised by haplo-diploid life cycles, and developing ovules are the organs in which the haploid and diploid generations coexist. Recently it has been shown that hormones such as auxin and cytokinins play important roles in ovule development and patterning. The establishment and regulation of auxin levels in cells is predominantly determined by the activity of the auxin efflux carrier proteins PIN-FORMED (PIN). To study the roles of PIN1 and PIN3 during ovule development we have used mutant alleles of both genes and also perturbed PIN1 and PIN3 expression using micro-RNAs controlled by the ovule specific DEFH9 (DEFIFICENS Homologue 9) promoter. PIN1 down-regulation and pin1-5 mutation severely affect female gametophyte development since embryo sacs arrest at the mono- and/or bi-nuclear stages (FG1 and FG3 stage). PIN3 function is not required for ovule development in wild-type or PIN1-silenced plants. We show that sporophytically expressed PIN1 is required for megagametogenesis, suggesting that sporophytic auxin flux might control the early stages of female gametophyte development, although auxin response is not visible in developing embryo sacs.
format	article
author	Luca Ceccato Simona Masiero Dola Sinha Roy Stefano Bencivenga Irma Roig-Villanova Franck Anicet Ditengou Klaus Palme Rüdiger Simon Lucia Colombo
author_facet	Luca Ceccato Simona Masiero Dola Sinha Roy Stefano Bencivenga Irma Roig-Villanova Franck Anicet Ditengou Klaus Palme Rüdiger Simon Lucia Colombo
author_sort	Luca Ceccato
title	Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.
title_short	Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.
title_full	Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.
title_fullStr	Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.
title_full_unstemmed	Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.
title_sort	maternal control of pin1 is required for female gametophyte development in arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/805eb194da7e49c5b9a2b404783e8caa
work_keys_str_mv	AT lucaceccato maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT simonamasiero maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT dolasinharoy maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT stefanobencivenga maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT irmaroigvillanova maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT franckanicetditengou maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT klauspalme maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT rudigersimon maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis AT luciacolombo maternalcontrolofpin1isrequiredforfemalegametophytedevelopmentinarabidopsis
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Maternal control of PIN1 is required for female gametophyte development in Arabidopsis.

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