Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype

Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype

Yong Bi,1– 3 Xiaobin Lin,4 Huazheng Liang,2 Dehao Yang,4 Xiaowei Zhang,4 Jianming Ke,4 Jingjing Xiao,2 Zhilin Chen,2 Weian Chen,4 Xu Zhang,4 Shaoshi Wang,2 Chun-Feng Liu1,3 1Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital...

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Autores principales: Bi Y, Lin X, Liang H, Yang D, Zhang X, Ke J, Xiao J, Chen Z, Chen W, Wang S, Liu CF
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2020
Materias:
parkinson’s disease
adipose-derived mesenchymal stem cells
cd4+t cell
t helper 17 cell
t regulatory cell
peripheral blood mononuclear cells
leukemia inhibitory factor
Geriatrics
RC952-954.6
Acceso en línea:https://doaj.org/article/806396e89e894d37adc522f6189a2274
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id oai:doaj.org-article:806396e89e894d37adc522f6189a2274
record_format dspace
institution DOAJ
collection DOAJ
language EN
topic parkinson’s disease
adipose-derived mesenchymal stem cells
cd4+t cell
t helper 17 cell
t regulatory cell
peripheral blood mononuclear cells
leukemia inhibitory factor
Geriatrics
RC952-954.6
spellingShingle parkinson’s disease
adipose-derived mesenchymal stem cells
cd4+t cell
t helper 17 cell
t regulatory cell
peripheral blood mononuclear cells
leukemia inhibitory factor
Geriatrics
RC952-954.6
Bi Y
Lin X
Liang H
Yang D
Zhang X
Ke J
Xiao J
Chen Z
Chen W
Zhang X
Wang S
Liu CF
Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype
description Yong Bi,1– 3 Xiaobin Lin,4 Huazheng Liang,2 Dehao Yang,4 Xiaowei Zhang,4 Jianming Ke,4 Jingjing Xiao,2 Zhilin Chen,2 Weian Chen,4 Xu Zhang,4 Shaoshi Wang,2 Chun-Feng Liu1,3 1Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China; 2Department of Neurology, Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine, Shanghai, People’s Republic of China; 3Institute of Neuroscience, Soochow University, Suzhou, People’s Republic of China; 4Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People’s Republic of ChinaCorrespondence: Chun-Feng Liu Email liuchunfeng@suda.edu.cnBackground: Parkinson’s disease (PD) is a neurodegenerative disorder displaying a typical neuroinflammation pathology that may result from an imbalance between regulatory T cells (Treg) and T helper 17 (Th17) cells. Human adipose tissue-derived mesenchymal stem cells (Ad-MSCs) exert immunomodulatory effects by inhibiting effector T cell responses and have been used to treat diverse immune disorders. We aimed to investigate the modulating effect of human Ad-MSCs on peripheral blood mononuclear cells (PBMCs) of patients with PD, focusing on differentiation into Th17 and Treg cells.Methods: We isolated human peripheral blood CD4+T cells and co-cultured them with Ad-MSCs at a ratio of 4:1 under either Th17 or Treg cell polarizing conditions for 4 days to detect the proportions of IL-17-producing CD4+T (Th17) and CD4+CD25+Foxp3+regulatory T (Treg) cells by flow cytometry. We also determined the mRNA expression levels of the retinoid-related orphan nuclear receptor (RORγt) transcription factor and those of interleukin-6 receptor (IL-6R), interleukin-23 receptor (IL-23R), leukemia inhibitory factor (LIF), and LIF receptor (LIFR) by quantitative reverse transcription PCR. We detected levels of cytokines in the supernatant (including LIF, IL-6, IL-23, IL-10, and TGF-β) using ELISA.Results: Our results showed that Ad-MSCs specifically inhibited the differentiation of PBMCs of patients with PD into IL-17-producing CD4+T cells by decreasing expressions of IL-6R, IL-23R, and RORγt (the key transcription factor for Th17 cells). Moreover, Ad-MSCs induced a functional CD4+CD25+Foxp3+T regulatory cell phenotype as evidenced by the secretion of IL-10. The levels of IL-6, IL-23, and TGF-β remained constant after co-culture under either the Th17 or the Treg cell polarizing condition. In addition, levels of LIF protein and its receptor mRNA were significantly increased under both polarizing conditions.Conclusion: The present in vitro study found that Ad-MSCs from healthy participants were able to correct the imbalance between Th17 and Treg found in PBMCs of PD patients, which were correlated with an increase in LIF secretion and a decrease in expression of IL-6R, IL-23R, and RORγt. These findings should be confirmed by in vivo experiments.Keywords: Parkinson’s disease, adipose-derived mesenchymal stem cells, CD4+T cell, T helper 17 cell, T regulatory cell, peripheral blood mononuclear cells, leukemia inhibitory factor
format article
author Bi Y
Lin X
Liang H
Yang D
Zhang X
Ke J
Xiao J
Chen Z
Chen W
Zhang X
Wang S
Liu CF
author_facet Bi Y
Lin X
Liang H
Yang D
Zhang X
Ke J
Xiao J
Chen Z
Chen W
Zhang X
Wang S
Liu CF
author_sort Bi Y
title Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype
title_short Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype
title_full Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype
title_fullStr Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype
title_full_unstemmed Human Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype
title_sort human adipose tissue-derived mesenchymal stem cells in parkinson’s disease: inhibition of t helper 17 cell differentiation and regulation of immune balance towards a regulatory t cell phenotype
publisher Dove Medical Press
publishDate 2020
url https://doaj.org/article/806396e89e894d37adc522f6189a2274
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spelling oai:doaj.org-article:806396e89e894d37adc522f6189a22742021-12-02T11:08:22ZHuman Adipose Tissue-Derived Mesenchymal Stem Cells in Parkinson’s Disease: Inhibition of T Helper 17 Cell Differentiation and Regulation of Immune Balance Towards a Regulatory T Cell Phenotype1178-1998https://doaj.org/article/806396e89e894d37adc522f6189a22742020-08-01T00:00:00Zhttps://www.dovepress.com/human-adipose-tissue-derived-mesenchymal-stem-cells-in-parkinsonrsquos-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Yong Bi,1– 3 Xiaobin Lin,4 Huazheng Liang,2 Dehao Yang,4 Xiaowei Zhang,4 Jianming Ke,4 Jingjing Xiao,2 Zhilin Chen,2 Weian Chen,4 Xu Zhang,4 Shaoshi Wang,2 Chun-Feng Liu1,3 1Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China; 2Department of Neurology, Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine, Shanghai, People’s Republic of China; 3Institute of Neuroscience, Soochow University, Suzhou, People’s Republic of China; 4Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People’s Republic of ChinaCorrespondence: Chun-Feng Liu Email liuchunfeng@suda.edu.cnBackground: Parkinson’s disease (PD) is a neurodegenerative disorder displaying a typical neuroinflammation pathology that may result from an imbalance between regulatory T cells (Treg) and T helper 17 (Th17) cells. Human adipose tissue-derived mesenchymal stem cells (Ad-MSCs) exert immunomodulatory effects by inhibiting effector T cell responses and have been used to treat diverse immune disorders. We aimed to investigate the modulating effect of human Ad-MSCs on peripheral blood mononuclear cells (PBMCs) of patients with PD, focusing on differentiation into Th17 and Treg cells.Methods: We isolated human peripheral blood CD4+T cells and co-cultured them with Ad-MSCs at a ratio of 4:1 under either Th17 or Treg cell polarizing conditions for 4 days to detect the proportions of IL-17-producing CD4+T (Th17) and CD4+CD25+Foxp3+regulatory T (Treg) cells by flow cytometry. We also determined the mRNA expression levels of the retinoid-related orphan nuclear receptor (RORγt) transcription factor and those of interleukin-6 receptor (IL-6R), interleukin-23 receptor (IL-23R), leukemia inhibitory factor (LIF), and LIF receptor (LIFR) by quantitative reverse transcription PCR. We detected levels of cytokines in the supernatant (including LIF, IL-6, IL-23, IL-10, and TGF-β) using ELISA.Results: Our results showed that Ad-MSCs specifically inhibited the differentiation of PBMCs of patients with PD into IL-17-producing CD4+T cells by decreasing expressions of IL-6R, IL-23R, and RORγt (the key transcription factor for Th17 cells). Moreover, Ad-MSCs induced a functional CD4+CD25+Foxp3+T regulatory cell phenotype as evidenced by the secretion of IL-10. The levels of IL-6, IL-23, and TGF-β remained constant after co-culture under either the Th17 or the Treg cell polarizing condition. In addition, levels of LIF protein and its receptor mRNA were significantly increased under both polarizing conditions.Conclusion: The present in vitro study found that Ad-MSCs from healthy participants were able to correct the imbalance between Th17 and Treg found in PBMCs of PD patients, which were correlated with an increase in LIF secretion and a decrease in expression of IL-6R, IL-23R, and RORγt. These findings should be confirmed by in vivo experiments.Keywords: Parkinson’s disease, adipose-derived mesenchymal stem cells, CD4+T cell, T helper 17 cell, T regulatory cell, peripheral blood mononuclear cells, leukemia inhibitory factorBi YLin XLiang HYang DZhang XKe JXiao JChen ZChen WZhang XWang SLiu CFDove Medical Pressarticleparkinson’s diseaseadipose-derived mesenchymal stem cellscd4+t cellt helper 17 cellt regulatory cellperipheral blood mononuclear cellsleukemia inhibitory factorGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 15, Pp 1383-1391 (2020)

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