Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells

Glutamate excitotoxicity is considered one of the major causes of retinal ganglion cell death in many retinal diseases. Retinal ganglion cell degeneration causes severe blindness since visual signals from the eye to the brain are conducted only through retinal ganglion cells. Objective: We aimed to...

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Autores principales:	Al-Dbass Abeer, Amina Musarat, Al Musayeib Nawal M., El-Anssary Amira A., Bhat Ramesa Shafi, Fahmy Rania, Alhamdan Majd M., El-Ansary Afaf
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	lepidium sativum glutamate excitotoxicity retinal ganglion cell cell viability comet assay Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/8063df7f121c41b685fdcf69c085c6b8
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spelling	oai:doaj.org-article:8063df7f121c41b685fdcf69c085c6b82021-12-05T14:11:05ZLepidium sativum as candidate against excitotoxicity in retinal ganglion cells2081-693610.1515/tnsci-2020-0174https://doaj.org/article/8063df7f121c41b685fdcf69c085c6b82021-06-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2020-0174https://doaj.org/toc/2081-6936Glutamate excitotoxicity is considered one of the major causes of retinal ganglion cell death in many retinal diseases. Retinal ganglion cell degeneration causes severe blindness since visual signals from the eye to the brain are conducted only through retinal ganglion cells. Objective: We aimed to explore the potential ameliorative effects of L. sativum against glutamate excitotoxicity-induced retinal ganglion cell damage. Methods: Pure retinal ganglion cells were divided into a control group (untreated); L. sativum-treated groups in which retinal ganglion cells were treated with 5, 10, 50, or 100 µg/mL L. sativum seed extract for 2 h; glutamate-treated groups in which cells were treated with 5, 10, 50, or 100 µM glutamate for 48 h; and L. sativum/glutamate groups [pretreatment with L. sativum for 2 h (50 or 100 µg/mL) before glutamate treatment at 100 µM for 48 h]. Cell damage was assessed by comet assay and cell viability was by MTT test. Results: Tailed DNA, tail length, and tail moment of the 50 and 100 mM glutamate-treated groups were significantly greater than those of the blank control group, while the L. sativum-treated groups demonstrated nonsignificantly different tailed DNA, tail length, and tail moment compared with the blank control group, but significantly lower values compared with the glutamate-treated groups. Conclusion: L. sativum ameliorated the cell viability in retinal ganglion cells after high-concentration glutamate exposure. L. sativum seed extracts were efficient anti-excitotoxic and antioxidant agent that might improve the clinical presentation of many neurological disorders.Al-Dbass AbeerAmina MusaratAl Musayeib Nawal M.El-Anssary Amira A.Bhat Ramesa ShafiFahmy RaniaAlhamdan Majd M.El-Ansary AfafDe Gruyterarticlelepidium sativumglutamate excitotoxicityretinal ganglion cellcell viabilitycomet assayNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 12, Iss 1, Pp 247-259 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lepidium sativum glutamate excitotoxicity retinal ganglion cell cell viability comet assay Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	lepidium sativum glutamate excitotoxicity retinal ganglion cell cell viability comet assay Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Al-Dbass Abeer Amina Musarat Al Musayeib Nawal M. El-Anssary Amira A. Bhat Ramesa Shafi Fahmy Rania Alhamdan Majd M. El-Ansary Afaf Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells
description	Glutamate excitotoxicity is considered one of the major causes of retinal ganglion cell death in many retinal diseases. Retinal ganglion cell degeneration causes severe blindness since visual signals from the eye to the brain are conducted only through retinal ganglion cells. Objective: We aimed to explore the potential ameliorative effects of L. sativum against glutamate excitotoxicity-induced retinal ganglion cell damage. Methods: Pure retinal ganglion cells were divided into a control group (untreated); L. sativum-treated groups in which retinal ganglion cells were treated with 5, 10, 50, or 100 µg/mL L. sativum seed extract for 2 h; glutamate-treated groups in which cells were treated with 5, 10, 50, or 100 µM glutamate for 48 h; and L. sativum/glutamate groups [pretreatment with L. sativum for 2 h (50 or 100 µg/mL) before glutamate treatment at 100 µM for 48 h]. Cell damage was assessed by comet assay and cell viability was by MTT test. Results: Tailed DNA, tail length, and tail moment of the 50 and 100 mM glutamate-treated groups were significantly greater than those of the blank control group, while the L. sativum-treated groups demonstrated nonsignificantly different tailed DNA, tail length, and tail moment compared with the blank control group, but significantly lower values compared with the glutamate-treated groups. Conclusion: L. sativum ameliorated the cell viability in retinal ganglion cells after high-concentration glutamate exposure. L. sativum seed extracts were efficient anti-excitotoxic and antioxidant agent that might improve the clinical presentation of many neurological disorders.
format	article
author	Al-Dbass Abeer Amina Musarat Al Musayeib Nawal M. El-Anssary Amira A. Bhat Ramesa Shafi Fahmy Rania Alhamdan Majd M. El-Ansary Afaf
author_facet	Al-Dbass Abeer Amina Musarat Al Musayeib Nawal M. El-Anssary Amira A. Bhat Ramesa Shafi Fahmy Rania Alhamdan Majd M. El-Ansary Afaf
author_sort	Al-Dbass Abeer
title	Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells
title_short	Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells
title_full	Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells
title_fullStr	Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells
title_full_unstemmed	Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells
title_sort	lepidium sativum as candidate against excitotoxicity in retinal ganglion cells
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/8063df7f121c41b685fdcf69c085c6b8
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Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells

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