Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide

Glioblastoma (GBM) is the most common malignant primary brain tumor, and GBM patients have a poor overall prognosis. CDC20 expression is increased in a variety of tumors and associated with temozolomide (TMZ) resistance in glioma cells. Apcin specifically binds to CDC20 to inhibit APC/C-CDC20 intera...

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Autores principales:	Yiming Ding, Chuanbao Zhang, Lei He, Xinyu Song, Chengjun Zheng, Yuchu Pan, Shuqing Yu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	glioblastoma cdc20 apcin temozolomide Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/8068b882860c4b75a9f0f754d9266177
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spelling	oai:doaj.org-article:8068b882860c4b75a9f0f754d92661772021-12-01T14:41:00ZApcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide2165-59792165-598710.1080/21655979.2021.2003927https://doaj.org/article/8068b882860c4b75a9f0f754d92661772021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2003927https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Glioblastoma (GBM) is the most common malignant primary brain tumor, and GBM patients have a poor overall prognosis. CDC20 expression is increased in a variety of tumors and associated with temozolomide (TMZ) resistance in glioma cells. Apcin specifically binds to CDC20 to inhibit APC/C-CDC20 interaction and exhibits antitumor properties. The purpose of this article was to assess whether apcin inhibits tumor growth in glioma cell lines and increases the sensitivity of GBM to TMZ. In this study, a series of biochemical assays, such as Cell Counting Kit-8 (CCK-8), wound healing, apoptosis and colony formation assays, were performed to determine the antitumor properties of apcin in glioma cells. GBM cell apoptosis was detected by western blotting analysis of related proteins. Apcin increased the sensitivity of glioma to TMZ, as confirmed by CCK-8 and western blotting analysis. The results showed that apcin significantly inhibited the proliferation of glioma cells in a time- and dose-dependent manner. The migration decreased with increasing apcin concentrations. Increased Bim expression indicated that apcin promotes the apoptosis of glioma cells. Furthermore, apcin improved glioma sensitivity to TMZ. The results showed that apcin can effectively inhibit GBM growth and improve TMZ sensitivity. Apcin has the potential to treat GBM and is expected to provide new ideas for individualized treatment.Yiming DingChuanbao ZhangLei HeXinyu SongChengjun ZhengYuchu PanShuqing YuTaylor & Francis Grouparticleglioblastomacdc20apcintemozolomideBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 10791-10798 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioblastoma cdc20 apcin temozolomide Biotechnology TP248.13-248.65
spellingShingle	glioblastoma cdc20 apcin temozolomide Biotechnology TP248.13-248.65 Yiming Ding Chuanbao Zhang Lei He Xinyu Song Chengjun Zheng Yuchu Pan Shuqing Yu Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide
description	Glioblastoma (GBM) is the most common malignant primary brain tumor, and GBM patients have a poor overall prognosis. CDC20 expression is increased in a variety of tumors and associated with temozolomide (TMZ) resistance in glioma cells. Apcin specifically binds to CDC20 to inhibit APC/C-CDC20 interaction and exhibits antitumor properties. The purpose of this article was to assess whether apcin inhibits tumor growth in glioma cell lines and increases the sensitivity of GBM to TMZ. In this study, a series of biochemical assays, such as Cell Counting Kit-8 (CCK-8), wound healing, apoptosis and colony formation assays, were performed to determine the antitumor properties of apcin in glioma cells. GBM cell apoptosis was detected by western blotting analysis of related proteins. Apcin increased the sensitivity of glioma to TMZ, as confirmed by CCK-8 and western blotting analysis. The results showed that apcin significantly inhibited the proliferation of glioma cells in a time- and dose-dependent manner. The migration decreased with increasing apcin concentrations. Increased Bim expression indicated that apcin promotes the apoptosis of glioma cells. Furthermore, apcin improved glioma sensitivity to TMZ. The results showed that apcin can effectively inhibit GBM growth and improve TMZ sensitivity. Apcin has the potential to treat GBM and is expected to provide new ideas for individualized treatment.
format	article
author	Yiming Ding Chuanbao Zhang Lei He Xinyu Song Chengjun Zheng Yuchu Pan Shuqing Yu
author_facet	Yiming Ding Chuanbao Zhang Lei He Xinyu Song Chengjun Zheng Yuchu Pan Shuqing Yu
author_sort	Yiming Ding
title	Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide
title_short	Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide
title_full	Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide
title_fullStr	Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide
title_full_unstemmed	Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide
title_sort	apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8068b882860c4b75a9f0f754d9266177
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Apcin inhibits the growth and invasion of glioblastoma cells and improves glioma sensitivity to temozolomide

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