Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members

Abstract Endothelial cell injury can promote the development of various cardiovascular diseases, thus, fully understanding the mechanisms underlying the maintenance of vascular endothelial cell homoeostasis may help prevent and treat cardiovascular disease. Kaiso, a zinc finger and BTB domain contai...

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Autores principales:	Xiaodong Xue, Jian Zhang, Huai Lan, Yinli Xu, Huishan Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/806a9cea9776495b9fd6c4f490abec0c
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spelling	oai:doaj.org-article:806a9cea9776495b9fd6c4f490abec0c2021-12-02T15:05:29ZKaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members10.1038/s41598-017-07559-02045-2322https://doaj.org/article/806a9cea9776495b9fd6c4f490abec0c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07559-0https://doaj.org/toc/2045-2322Abstract Endothelial cell injury can promote the development of various cardiovascular diseases, thus, fully understanding the mechanisms underlying the maintenance of vascular endothelial cell homoeostasis may help prevent and treat cardiovascular disease. Kaiso, a zinc finger and BTB domain containing transcription factor, is key to embryonic development and cancer, but how Kaiso interacts with vascular endothelium is not fully understood. We report that Kaiso has an anti-apoptotic function in human umbilical vein endothelial cells (HUVECs) and human microvascular endothelial cells (HMEC-1s). Overexpression of Kaiso significantly increased cell viability and inhibited hydrogen peroxide-induced apoptosis. Furthermore, Kaiso increased expression of B-cell CLL/lymphoma 2 (BCL2) and reduced expression of BCL2-associated X protein (BAX) and BCL2-interacting killer (BIK) by differentially regulating gene promoter activity. Methylated DNA and specific Kaiso binding site (KBS) contributed to gene regulatory activity of Kaiso. In addition, p120ctn functioned cooperatively in Kaiso-mediated transcriptional regulation.Xiaodong XueJian ZhangHuai LanYinli XuHuishan WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaodong Xue Jian Zhang Huai Lan Yinli Xu Huishan Wang Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members
description	Abstract Endothelial cell injury can promote the development of various cardiovascular diseases, thus, fully understanding the mechanisms underlying the maintenance of vascular endothelial cell homoeostasis may help prevent and treat cardiovascular disease. Kaiso, a zinc finger and BTB domain containing transcription factor, is key to embryonic development and cancer, but how Kaiso interacts with vascular endothelium is not fully understood. We report that Kaiso has an anti-apoptotic function in human umbilical vein endothelial cells (HUVECs) and human microvascular endothelial cells (HMEC-1s). Overexpression of Kaiso significantly increased cell viability and inhibited hydrogen peroxide-induced apoptosis. Furthermore, Kaiso increased expression of B-cell CLL/lymphoma 2 (BCL2) and reduced expression of BCL2-associated X protein (BAX) and BCL2-interacting killer (BIK) by differentially regulating gene promoter activity. Methylated DNA and specific Kaiso binding site (KBS) contributed to gene regulatory activity of Kaiso. In addition, p120ctn functioned cooperatively in Kaiso-mediated transcriptional regulation.
format	article
author	Xiaodong Xue Jian Zhang Huai Lan Yinli Xu Huishan Wang
author_facet	Xiaodong Xue Jian Zhang Huai Lan Yinli Xu Huishan Wang
author_sort	Xiaodong Xue
title	Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members
title_short	Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members
title_full	Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members
title_fullStr	Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members
title_full_unstemmed	Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members
title_sort	kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of b-cell cll/lymphoma 2 family members
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/806a9cea9776495b9fd6c4f490abec0c
work_keys_str_mv	AT xiaodongxue kaisoprotectshumanumbilicalveinendothelialcellsagainstapoptosisbydifferentiallyregulatingtheexpressionofbcellclllymphoma2familymembers AT jianzhang kaisoprotectshumanumbilicalveinendothelialcellsagainstapoptosisbydifferentiallyregulatingtheexpressionofbcellclllymphoma2familymembers AT huailan kaisoprotectshumanumbilicalveinendothelialcellsagainstapoptosisbydifferentiallyregulatingtheexpressionofbcellclllymphoma2familymembers AT yinlixu kaisoprotectshumanumbilicalveinendothelialcellsagainstapoptosisbydifferentiallyregulatingtheexpressionofbcellclllymphoma2familymembers AT huishanwang kaisoprotectshumanumbilicalveinendothelialcellsagainstapoptosisbydifferentiallyregulatingtheexpressionofbcellclllymphoma2familymembers
_version_	1718388842071851008

Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members

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