Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China
<i>Enterocytozoon bieneusi</i> is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of <i>E. bieneusi</i> has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution...
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| Main Authors: | , , , , , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
MDPI AG
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/806dcad2f92b4deeb6db586a6480f701 |
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| Summary: | <i>Enterocytozoon bieneusi</i> is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of <i>E. bieneusi</i> has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution of <i>E. bieneusi</i> in dairy cattle in Yunnan province in China is still absent. In this study, 490 Holstein Cows and 351 dairy buffalo fecal samples were collected from three regions in Yunnan province, China. By using nest-PCR that targets the internal transcribed spacer (ITS), we found that the prevalence of <i>E. bieneusi</i> was 0.59% (5/841). DNA sequence analysis showed that five <i>E. bieneusi</i> genotypes were identified in this study, including two novel genotypes, YNDCEB-90 and YNDCEB-174, and three known genotypes (I, J, BEB4). Phylogenetic analysis revealed that two novel genotypes, YNDCEB-90 and YNDCEB-174, were clustered into Group 1, representing the zoonotic potential. The remaining genotypes I, J, and BEB4, which are the most frequent genotypes of <i>E. bieneusi</i> infection in cattle and lead to <i>E. bieneusi</i> infection in humans, belonged to Group 2. Although the lower prevalence of <i>E. bieneusi</i> was detected in dairy cattle in Yunnan province, it indicates that dairy cattle should be considered to be one of the potential hosts for transmitting <i>E. bieneusi</i> to humans. These findings are important for the development of effective prevention strategies for microsporidiosis. |
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