Prevalence and Novel Genotypes Identification of Enterocytozoon bieneusi in Dairy Cattle in Yunnan Province, China

Enterocytozoon bieneusi is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of E. bieneusi has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution...

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Autores principales:	Hai-Yang Song, Kai-Sheng Wang, Jian-Fa Yang, Hua-Ming Mao, Li-Hua Pu, Yang Zou, Jun Ma, Xing-Quan Zhu, Feng-Cai Zou, Jun-Jun He
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dairy cattle <i>Enterocytozoon bieneusi</i> prevalence zoonotic potential Yunnan province China Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/806dcad2f92b4deeb6db586a6480f701
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spelling	oai:doaj.org-article:806dcad2f92b4deeb6db586a6480f7012021-11-25T16:13:35ZPrevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China10.3390/ani111130142076-2615https://doaj.org/article/806dcad2f92b4deeb6db586a6480f7012021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3014https://doaj.org/toc/2076-2615<i>Enterocytozoon bieneusi</i> is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of <i>E. bieneusi</i> has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution of <i>E. bieneusi</i> in dairy cattle in Yunnan province in China is still absent. In this study, 490 Holstein Cows and 351 dairy buffalo fecal samples were collected from three regions in Yunnan province, China. By using nest-PCR that targets the internal transcribed spacer (ITS), we found that the prevalence of <i>E. bieneusi</i> was 0.59% (5/841). DNA sequence analysis showed that five <i>E. bieneusi</i> genotypes were identified in this study, including two novel genotypes, YNDCEB-90 and YNDCEB-174, and three known genotypes (I, J, BEB4). Phylogenetic analysis revealed that two novel genotypes, YNDCEB-90 and YNDCEB-174, were clustered into Group 1, representing the zoonotic potential. The remaining genotypes I, J, and BEB4, which are the most frequent genotypes of <i>E. bieneusi</i> infection in cattle and lead to <i>E. bieneusi</i> infection in humans, belonged to Group 2. Although the lower prevalence of <i>E. bieneusi</i> was detected in dairy cattle in Yunnan province, it indicates that dairy cattle should be considered to be one of the potential hosts for transmitting <i>E. bieneusi</i> to humans. These findings are important for the development of effective prevention strategies for microsporidiosis.Hai-Yang SongKai-Sheng WangJian-Fa YangHua-Ming MaoLi-Hua PuYang ZouJun MaXing-Quan ZhuFeng-Cai ZouJun-Jun HeMDPI AGarticledairy cattle<i>Enterocytozoon bieneusi</i>prevalencezoonotic potentialYunnan provinceChinaVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3014, p 3014 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dairy cattle <i>Enterocytozoon bieneusi</i> prevalence zoonotic potential Yunnan province China Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	dairy cattle <i>Enterocytozoon bieneusi</i> prevalence zoonotic potential Yunnan province China Veterinary medicine SF600-1100 Zoology QL1-991 Hai-Yang Song Kai-Sheng Wang Jian-Fa Yang Hua-Ming Mao Li-Hua Pu Yang Zou Jun Ma Xing-Quan Zhu Feng-Cai Zou Jun-Jun He Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China
description	<i>Enterocytozoon bieneusi</i> is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of <i>E. bieneusi</i> has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution of <i>E. bieneusi</i> in dairy cattle in Yunnan province in China is still absent. In this study, 490 Holstein Cows and 351 dairy buffalo fecal samples were collected from three regions in Yunnan province, China. By using nest-PCR that targets the internal transcribed spacer (ITS), we found that the prevalence of <i>E. bieneusi</i> was 0.59% (5/841). DNA sequence analysis showed that five <i>E. bieneusi</i> genotypes were identified in this study, including two novel genotypes, YNDCEB-90 and YNDCEB-174, and three known genotypes (I, J, BEB4). Phylogenetic analysis revealed that two novel genotypes, YNDCEB-90 and YNDCEB-174, were clustered into Group 1, representing the zoonotic potential. The remaining genotypes I, J, and BEB4, which are the most frequent genotypes of <i>E. bieneusi</i> infection in cattle and lead to <i>E. bieneusi</i> infection in humans, belonged to Group 2. Although the lower prevalence of <i>E. bieneusi</i> was detected in dairy cattle in Yunnan province, it indicates that dairy cattle should be considered to be one of the potential hosts for transmitting <i>E. bieneusi</i> to humans. These findings are important for the development of effective prevention strategies for microsporidiosis.
format	article
author	Hai-Yang Song Kai-Sheng Wang Jian-Fa Yang Hua-Ming Mao Li-Hua Pu Yang Zou Jun Ma Xing-Quan Zhu Feng-Cai Zou Jun-Jun He
author_facet	Hai-Yang Song Kai-Sheng Wang Jian-Fa Yang Hua-Ming Mao Li-Hua Pu Yang Zou Jun Ma Xing-Quan Zhu Feng-Cai Zou Jun-Jun He
author_sort	Hai-Yang Song
title	Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China
title_short	Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China
title_full	Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China
title_fullStr	Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China
title_full_unstemmed	Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China
title_sort	prevalence and novel genotypes identification of <i>enterocytozoon bieneusi</i> in dairy cattle in yunnan province, china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/806dcad2f92b4deeb6db586a6480f701
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Prevalence and Novel Genotypes Identification of <i>Enterocytozoon bieneusi</i> in Dairy Cattle in Yunnan Province, China

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