Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling

Androgen receptor (AR) signaling is essential for maintaining spermatogenesis and male fertility. However, the molecular mechanisms by which AR acts between male germ cells and somatic cells during spermatogenesis have not begun to be revealed until recently. With the advances obtained from the use...

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Autores principales:	Congcong Cao, Qian Ma, Shaomei Mo, Ge Shu, Qunlong Liu, Jing Ye, Yaoting Gui
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	spermatogenesis AR scRNA-seq male infertility knockout Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/807224fc1ec445e8a3b5929880368353
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spelling	oai:doaj.org-article:807224fc1ec445e8a3b59298803683532021-11-15T05:58:05ZSingle-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling2296-634X10.3389/fcell.2021.763267https://doaj.org/article/807224fc1ec445e8a3b59298803683532021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.763267/fullhttps://doaj.org/toc/2296-634XAndrogen receptor (AR) signaling is essential for maintaining spermatogenesis and male fertility. However, the molecular mechanisms by which AR acts between male germ cells and somatic cells during spermatogenesis have not begun to be revealed until recently. With the advances obtained from the use of transgenic mice lacking AR in Sertoli cells (SCARKO) and single-cell transcriptomic sequencing (scRNA-seq), the cell specific targets of AR action as well as the genes and signaling pathways that are regulated by AR are being identified. In this study, we collected scRNA-seq data from wild-type (WT) and SCARKO mice testes at p20 and identified four somatic cell populations and two male germ cell populations. Further analysis identified that the distribution of Sertoli cells was completely different and uncovered the cellular heterogeneity and transcriptional changes between WT and SCARKO Sertoli cells. In addition, several differentially expressed genes (DEGs) in SCARKO Sertoli cells, many of which have been previously implicated in cell cycle, apoptosis and male infertility, have also been identified. Together, our research explores a novel perspective on the changes in the transcription level of various cell types between WT and SCARKO mice testes, providing new insights for the investigations of the molecular and cellular processes regulated by AR signaling in Sertoli cells.Congcong CaoQian MaShaomei MoGe ShuQunlong LiuJing YeYaoting GuiFrontiers Media S.A.articlespermatogenesisARscRNA-seqmale infertilityknockoutBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	spermatogenesis AR scRNA-seq male infertility knockout Biology (General) QH301-705.5
spellingShingle	spermatogenesis AR scRNA-seq male infertility knockout Biology (General) QH301-705.5 Congcong Cao Qian Ma Shaomei Mo Ge Shu Qunlong Liu Jing Ye Yaoting Gui Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling
description	Androgen receptor (AR) signaling is essential for maintaining spermatogenesis and male fertility. However, the molecular mechanisms by which AR acts between male germ cells and somatic cells during spermatogenesis have not begun to be revealed until recently. With the advances obtained from the use of transgenic mice lacking AR in Sertoli cells (SCARKO) and single-cell transcriptomic sequencing (scRNA-seq), the cell specific targets of AR action as well as the genes and signaling pathways that are regulated by AR are being identified. In this study, we collected scRNA-seq data from wild-type (WT) and SCARKO mice testes at p20 and identified four somatic cell populations and two male germ cell populations. Further analysis identified that the distribution of Sertoli cells was completely different and uncovered the cellular heterogeneity and transcriptional changes between WT and SCARKO Sertoli cells. In addition, several differentially expressed genes (DEGs) in SCARKO Sertoli cells, many of which have been previously implicated in cell cycle, apoptosis and male infertility, have also been identified. Together, our research explores a novel perspective on the changes in the transcription level of various cell types between WT and SCARKO mice testes, providing new insights for the investigations of the molecular and cellular processes regulated by AR signaling in Sertoli cells.
format	article
author	Congcong Cao Qian Ma Shaomei Mo Ge Shu Qunlong Liu Jing Ye Yaoting Gui
author_facet	Congcong Cao Qian Ma Shaomei Mo Ge Shu Qunlong Liu Jing Ye Yaoting Gui
author_sort	Congcong Cao
title	Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling
title_short	Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling
title_full	Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling
title_fullStr	Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling
title_full_unstemmed	Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling
title_sort	single-cell rna sequencing defines the regulation of spermatogenesis by sertoli-cell androgen signaling
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/807224fc1ec445e8a3b5929880368353
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Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling

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