Molecular principles of recruitment and dynamics of guest proteins in liquid droplets

Abstract Despite the continuous discovery of host and guest proteins in membraneless organelles, complex host–guest interactions hinder the understanding of the molecular grammar governing liquid–liquid phase separation. In this study, we characterized the localization and dynamic properties of gues...

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Autores principales:	Kiyoto Kamagata, Nanako Iwaki, Milan Kumar Hazra, Saori Kanbayashi, Trishit Banerjee, Rika Chiba, Seiji Sakomoto, Virginie Gaudon, Bertrand Castaing, Hiroto Takahashi, Michiko Kimura, Hiroyuki Oikawa, Satoshi Takahashi, Yaakov Levy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8076c09dbce8459386b631ea88ef4285
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spelling	oai:doaj.org-article:8076c09dbce8459386b631ea88ef42852021-12-02T19:16:54ZMolecular principles of recruitment and dynamics of guest proteins in liquid droplets10.1038/s41598-021-98955-02045-2322https://doaj.org/article/8076c09dbce8459386b631ea88ef42852021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98955-0https://doaj.org/toc/2045-2322Abstract Despite the continuous discovery of host and guest proteins in membraneless organelles, complex host–guest interactions hinder the understanding of the molecular grammar governing liquid–liquid phase separation. In this study, we characterized the localization and dynamic properties of guest proteins in liquid droplets using single-molecule fluorescence microscopy. Eighteen guest proteins of different sizes, structures, and oligomeric states were examined in host p53 liquid droplets. Recruitment did not significantly depend on the structural properties of the guest proteins, but was moderately correlated with their length, total charge, and number of R and Y residues. In contrast, the diffusion of disordered guest proteins was comparable to that of host p53, whereas that of folded proteins varied widely. Molecular dynamics simulations suggest that folded proteins diffuse within the voids of the liquid droplet while interacting weakly with neighboring host proteins, whereas disordered proteins adapt their structures to form tight interactions with the host proteins. Our study provides insights into the key molecular principles of the localization and dynamics of guest proteins in liquid droplets.Kiyoto KamagataNanako IwakiMilan Kumar HazraSaori KanbayashiTrishit BanerjeeRika ChibaSeiji SakomotoVirginie GaudonBertrand CastaingHiroto TakahashiMichiko KimuraHiroyuki OikawaSatoshi TakahashiYaakov LevyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kiyoto Kamagata Nanako Iwaki Milan Kumar Hazra Saori Kanbayashi Trishit Banerjee Rika Chiba Seiji Sakomoto Virginie Gaudon Bertrand Castaing Hiroto Takahashi Michiko Kimura Hiroyuki Oikawa Satoshi Takahashi Yaakov Levy Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
description	Abstract Despite the continuous discovery of host and guest proteins in membraneless organelles, complex host–guest interactions hinder the understanding of the molecular grammar governing liquid–liquid phase separation. In this study, we characterized the localization and dynamic properties of guest proteins in liquid droplets using single-molecule fluorescence microscopy. Eighteen guest proteins of different sizes, structures, and oligomeric states were examined in host p53 liquid droplets. Recruitment did not significantly depend on the structural properties of the guest proteins, but was moderately correlated with their length, total charge, and number of R and Y residues. In contrast, the diffusion of disordered guest proteins was comparable to that of host p53, whereas that of folded proteins varied widely. Molecular dynamics simulations suggest that folded proteins diffuse within the voids of the liquid droplet while interacting weakly with neighboring host proteins, whereas disordered proteins adapt their structures to form tight interactions with the host proteins. Our study provides insights into the key molecular principles of the localization and dynamics of guest proteins in liquid droplets.
format	article
author	Kiyoto Kamagata Nanako Iwaki Milan Kumar Hazra Saori Kanbayashi Trishit Banerjee Rika Chiba Seiji Sakomoto Virginie Gaudon Bertrand Castaing Hiroto Takahashi Michiko Kimura Hiroyuki Oikawa Satoshi Takahashi Yaakov Levy
author_facet	Kiyoto Kamagata Nanako Iwaki Milan Kumar Hazra Saori Kanbayashi Trishit Banerjee Rika Chiba Seiji Sakomoto Virginie Gaudon Bertrand Castaing Hiroto Takahashi Michiko Kimura Hiroyuki Oikawa Satoshi Takahashi Yaakov Levy
author_sort	Kiyoto Kamagata
title	Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
title_short	Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
title_full	Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
title_fullStr	Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
title_full_unstemmed	Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
title_sort	molecular principles of recruitment and dynamics of guest proteins in liquid droplets
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8076c09dbce8459386b631ea88ef4285
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_version_	1718376922830864384

Molecular principles of recruitment and dynamics of guest proteins in liquid droplets

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