Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft

Abstract Tissue engineered vascular grafts (TEVG) are potentially clear from ethical and epidemiological concerns sources for reconstructive surgery for small diameter blood vessels replacement. Here, we proposed a novel method to create three-layered TEVG on biocompatible glass fiber scaffolds star...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kazutomo Baba, Andrey Mikhailov, Yoshiyuki Sankai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8078d869d7714a8faf1ca5265763369b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8078d869d7714a8faf1ca5265763369b
record_format	dspace
spelling	oai:doaj.org-article:8078d869d7714a8faf1ca5265763369b2021-12-02T17:56:56ZDynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft10.1038/s41598-021-94023-92045-2322https://doaj.org/article/8078d869d7714a8faf1ca5265763369b2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94023-9https://doaj.org/toc/2045-2322Abstract Tissue engineered vascular grafts (TEVG) are potentially clear from ethical and epidemiological concerns sources for reconstructive surgery for small diameter blood vessels replacement. Here, we proposed a novel method to create three-layered TEVG on biocompatible glass fiber scaffolds starting from flat sheet state into tubular shape and to train the resulting tissue by our developed bioreactor system. Constructed tubular tissues were matured and trained under 3 types of individual flow programs, and their mechanical and biological properties were analyzed. Training in the bioreactor significantly increased the tissue burst pressure resistance (up to 18 kPa) comparing to untrained tissue. Fluorescent imaging and histological examination of trained vascular tissue revealed that each cell layer has its own individual response to training flow rates. Histological analysis suggested reverse relationship between tissue thickness and shear stress, and the thickness variation profiles were individual between all three types of cell layers. Concluding: a three-layered tissue structure similar to physiological can be assembled by seeding different cell types in succession; the following training of the formed tissue with increasing flow in a bioreactor is effective for promoting cell survival, improving pressure resistance, and cell layer formation of desired properties.Kazutomo BabaAndrey MikhailovYoshiyuki SankaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazutomo Baba Andrey Mikhailov Yoshiyuki Sankai Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft
description	Abstract Tissue engineered vascular grafts (TEVG) are potentially clear from ethical and epidemiological concerns sources for reconstructive surgery for small diameter blood vessels replacement. Here, we proposed a novel method to create three-layered TEVG on biocompatible glass fiber scaffolds starting from flat sheet state into tubular shape and to train the resulting tissue by our developed bioreactor system. Constructed tubular tissues were matured and trained under 3 types of individual flow programs, and their mechanical and biological properties were analyzed. Training in the bioreactor significantly increased the tissue burst pressure resistance (up to 18 kPa) comparing to untrained tissue. Fluorescent imaging and histological examination of trained vascular tissue revealed that each cell layer has its own individual response to training flow rates. Histological analysis suggested reverse relationship between tissue thickness and shear stress, and the thickness variation profiles were individual between all three types of cell layers. Concluding: a three-layered tissue structure similar to physiological can be assembled by seeding different cell types in succession; the following training of the formed tissue with increasing flow in a bioreactor is effective for promoting cell survival, improving pressure resistance, and cell layer formation of desired properties.
format	article
author	Kazutomo Baba Andrey Mikhailov Yoshiyuki Sankai
author_facet	Kazutomo Baba Andrey Mikhailov Yoshiyuki Sankai
author_sort	Kazutomo Baba
title	Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft
title_short	Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft
title_full	Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft
title_fullStr	Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft
title_full_unstemmed	Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft
title_sort	dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8078d869d7714a8faf1ca5265763369b
work_keys_str_mv	AT kazutomobaba dynamicflowprimingprogramsallowtuningupthecelllayerspropertiesforengineeredvasculargraft AT andreymikhailov dynamicflowprimingprogramsallowtuningupthecelllayerspropertiesforengineeredvasculargraft AT yoshiyukisankai dynamicflowprimingprogramsallowtuningupthecelllayerspropertiesforengineeredvasculargraft
_version_	1718379055419490304

Dynamic flow priming programs allow tuning up the cell layers properties for engineered vascular graft

Ejemplares similares