Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats

Introduction: Isoprenaline or isoproterenol (1-(3,4-dihydroxyphenyl)-2-isopropylaminoethanolhydrochloride; ISO), a synthetic b-adrenergic agonist, can be used to establish myocardial ischemia, cardiotoxicity, necrosis and/or an experimental model of infarction in rats. Aim: Determination of the dyna...

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Autores principales:	Gatarić Nina, Ilić Ana, Todorović Dušan, Mutavdžin Slavica, Jakovljević-Uzelac Jovana, Stanković Sanja, Đurić Dragan
Formato:	article
Lenguaje:	EN SR
Publicado:	University of Belgrade, Medical Faculty 2021
Materias:	acute myocardial injury isoprenaline cardiac injury biomarkers ecg rat Medicine R
Acceso en línea:	https://doaj.org/article/807e0d3a04144ff6b41f29b3f2cc754c
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spelling	oai:doaj.org-article:807e0d3a04144ff6b41f29b3f2cc754c2021-12-05T21:26:44ZFunctional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats0369-15272466-552510.5937/mp72-31512https://doaj.org/article/807e0d3a04144ff6b41f29b3f2cc754c2021-01-01T00:00:00Zhttps://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2021/0369-15272102011G.pdfhttps://doaj.org/toc/0369-1527https://doaj.org/toc/2466-5525Introduction: Isoprenaline or isoproterenol (1-(3,4-dihydroxyphenyl)-2-isopropylaminoethanolhydrochloride; ISO), a synthetic b-adrenergic agonist, can be used to establish myocardial ischemia, cardiotoxicity, necrosis and/or an experimental model of infarction in rats. Aim: Determination of the dynamics of myocardial injury biomarkers production of aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), and high-sensitive troponin T (hsTnT), with changes on electrocardiogram (ECG) parameters during the subcutaneous aplication of ISO in male Wistar rats. Material and methods: All animals (n = 23) were divided into two groups: control group (n = 11) treated with a saline solution, during two consecutive days (0,2 ml/kg b.m. daily, sc); and the ISO group (n = 12) treated with isoprenaline, during two consecutive days (85 mg/kg b.m. daily, sc). Blood was drawn from the rat tail vein in both groups, in order to determine serum activity levels of myocardial injury biomarkers, and an ECG (n = 6) was registered prior to the application, as well as 48h following the first dose of of saline solution or isoprenaline. Results: In comparison to the control group, in which no significant enzyme activities elevation (p > 0.05) nor ECG changes were registered, ISO group presented a significant rise of two clinically significant biomarkers of acute myocardial injury/myocardial infarction (AMI), CK (p = 0.05) and hsTnT (p < 0.01), as well as an ST segment elevation, with a patognomonic ECG change. Conclusion: Obtained results support previous studies, proving that isoprenaline represents an adequate experimental model for myocardial injury/AMI induction, and a "golden standard" for evaluating potential cardioprotective effects of pharmacological and non-pharmacological therapeutic modalities, with the ultimate goal of lowering the degree of lesions and improving post-infarction myocardium function.Gatarić NinaIlić AnaTodorović DušanMutavdžin SlavicaJakovljević-Uzelac JovanaStanković SanjaĐurić DraganUniversity of Belgrade, Medical Facultyarticleacute myocardial injuryisoprenalinecardiac injury biomarkersecgratMedicineRENSRMedicinski Podmladak, Vol 72, Iss 2, Pp 11-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN SR
topic	acute myocardial injury isoprenaline cardiac injury biomarkers ecg rat Medicine R
spellingShingle	acute myocardial injury isoprenaline cardiac injury biomarkers ecg rat Medicine R Gatarić Nina Ilić Ana Todorović Dušan Mutavdžin Slavica Jakovljević-Uzelac Jovana Stanković Sanja Đurić Dragan Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats
description	Introduction: Isoprenaline or isoproterenol (1-(3,4-dihydroxyphenyl)-2-isopropylaminoethanolhydrochloride; ISO), a synthetic b-adrenergic agonist, can be used to establish myocardial ischemia, cardiotoxicity, necrosis and/or an experimental model of infarction in rats. Aim: Determination of the dynamics of myocardial injury biomarkers production of aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), and high-sensitive troponin T (hsTnT), with changes on electrocardiogram (ECG) parameters during the subcutaneous aplication of ISO in male Wistar rats. Material and methods: All animals (n = 23) were divided into two groups: control group (n = 11) treated with a saline solution, during two consecutive days (0,2 ml/kg b.m. daily, sc); and the ISO group (n = 12) treated with isoprenaline, during two consecutive days (85 mg/kg b.m. daily, sc). Blood was drawn from the rat tail vein in both groups, in order to determine serum activity levels of myocardial injury biomarkers, and an ECG (n = 6) was registered prior to the application, as well as 48h following the first dose of of saline solution or isoprenaline. Results: In comparison to the control group, in which no significant enzyme activities elevation (p > 0.05) nor ECG changes were registered, ISO group presented a significant rise of two clinically significant biomarkers of acute myocardial injury/myocardial infarction (AMI), CK (p = 0.05) and hsTnT (p < 0.01), as well as an ST segment elevation, with a patognomonic ECG change. Conclusion: Obtained results support previous studies, proving that isoprenaline represents an adequate experimental model for myocardial injury/AMI induction, and a "golden standard" for evaluating potential cardioprotective effects of pharmacological and non-pharmacological therapeutic modalities, with the ultimate goal of lowering the degree of lesions and improving post-infarction myocardium function.
format	article
author	Gatarić Nina Ilić Ana Todorović Dušan Mutavdžin Slavica Jakovljević-Uzelac Jovana Stanković Sanja Đurić Dragan
author_facet	Gatarić Nina Ilić Ana Todorović Dušan Mutavdžin Slavica Jakovljević-Uzelac Jovana Stanković Sanja Đurić Dragan
author_sort	Gatarić Nina
title	Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats
title_short	Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats
title_full	Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats
title_fullStr	Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats
title_full_unstemmed	Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats
title_sort	functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats
publisher	University of Belgrade, Medical Faculty
publishDate	2021
url	https://doaj.org/article/807e0d3a04144ff6b41f29b3f2cc754c
work_keys_str_mv	AT gataricnina functionaldynamicsofmyocardialinjurybiomarkersproductionduringacuteisoprenalinetreatmentinrats AT ilicana functionaldynamicsofmyocardialinjurybiomarkersproductionduringacuteisoprenalinetreatmentinrats AT todorovicdusan functionaldynamicsofmyocardialinjurybiomarkersproductionduringacuteisoprenalinetreatmentinrats AT mutavdzinslavica functionaldynamicsofmyocardialinjurybiomarkersproductionduringacuteisoprenalinetreatmentinrats AT jakovljevicuzelacjovana functionaldynamicsofmyocardialinjurybiomarkersproductionduringacuteisoprenalinetreatmentinrats AT stankovicsanja functionaldynamicsofmyocardialinjurybiomarkersproductionduringacuteisoprenalinetreatmentinrats AT đuricdragan functionaldynamicsofmyocardialinjurybiomarkersproductionduringacuteisoprenalinetreatmentinrats
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Functional dynamics of myocardial injury biomarkers production during acute isoprenaline treatment in rats

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