Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells

Schisandrin A (SchA) has been reported to have good anti-cancer effects. However, its anti-cancer mechanism in breast cancer remains unknown. This study aimed to explore the mechanism of SchA in breast cancer treatment using bio-informatics analysis and in vitro experiments. The Cancer Genome Atlas...

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Autores principales:	Ling Chen, Li-Quan Ren, Zhong Liu, Xin Liu, Han Tu, Xu-Ying Huang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	schisandrin a mda-mb-231 cells mechanism network pharmacology molecular docking in vitro experiments Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/80813fd0c30c4706b7efad5586a578c6
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spelling	oai:doaj.org-article:80813fd0c30c4706b7efad5586a578c62021-11-04T15:51:53ZBio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells2165-59792165-598710.1080/21655979.2021.1982307https://doaj.org/article/80813fd0c30c4706b7efad5586a578c62021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1982307https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Schisandrin A (SchA) has been reported to have good anti-cancer effects. However, its anti-cancer mechanism in breast cancer remains unknown. This study aimed to explore the mechanism of SchA in breast cancer treatment using bio-informatics analysis and in vitro experiments. The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Cards, and PharmMapper databases were used to screen the candidate targets of SchA against MDA-MB-231 cells selected as the tested cell line through MTT analysis. The functions and pathways of the targets were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and further analyzed using DAVID 6.8.1 database. Network pharmacology analysis revealed 77 candidate targets, 31 signal pathways, and 208 GO entries (P < 0.05). The targets regulated serine-type endopeptidase and protein tyrosine kinase activities, thereby promoting the migration and inhibiting the apoptosis of MDA-MB-231 cells. Comprehensive analysis of the ‘Protein–Protein Interaction’ (PPI) and ‘Component-Targets-Pathways’ (C-T-P) networks constructed using Cytoscape 3.7.1 software revealed four core targets: EGFR, PIK3R1, MMP9 and Caspase 3. Their docking scores with SchA were subsequently investigated through molecular docking. The wound healing, Hoechst 33342/PI, and western blot assays confirmed that SchA significantly down-regulated EGFR, PIK3R1, and MMP9, but up-regulated cleaved-caspase 3, thus inhibiting the migration and promoting the apoptosis of MDA-MB-231 cells. Reckoning the findings of the study, SchA is a potential adjuvant treatment for breast cancer.Ling ChenLi-Quan RenZhong LiuXin LiuHan TuXu-Ying HuangTaylor & Francis Grouparticleschisandrin amda-mb-231 cellsmechanismnetwork pharmacologymolecular dockingin vitro experimentsBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 7678-7693 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	schisandrin a mda-mb-231 cells mechanism network pharmacology molecular docking in vitro experiments Biotechnology TP248.13-248.65
spellingShingle	schisandrin a mda-mb-231 cells mechanism network pharmacology molecular docking in vitro experiments Biotechnology TP248.13-248.65 Ling Chen Li-Quan Ren Zhong Liu Xin Liu Han Tu Xu-Ying Huang Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells
description	Schisandrin A (SchA) has been reported to have good anti-cancer effects. However, its anti-cancer mechanism in breast cancer remains unknown. This study aimed to explore the mechanism of SchA in breast cancer treatment using bio-informatics analysis and in vitro experiments. The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Cards, and PharmMapper databases were used to screen the candidate targets of SchA against MDA-MB-231 cells selected as the tested cell line through MTT analysis. The functions and pathways of the targets were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and further analyzed using DAVID 6.8.1 database. Network pharmacology analysis revealed 77 candidate targets, 31 signal pathways, and 208 GO entries (P < 0.05). The targets regulated serine-type endopeptidase and protein tyrosine kinase activities, thereby promoting the migration and inhibiting the apoptosis of MDA-MB-231 cells. Comprehensive analysis of the ‘Protein–Protein Interaction’ (PPI) and ‘Component-Targets-Pathways’ (C-T-P) networks constructed using Cytoscape 3.7.1 software revealed four core targets: EGFR, PIK3R1, MMP9 and Caspase 3. Their docking scores with SchA were subsequently investigated through molecular docking. The wound healing, Hoechst 33342/PI, and western blot assays confirmed that SchA significantly down-regulated EGFR, PIK3R1, and MMP9, but up-regulated cleaved-caspase 3, thus inhibiting the migration and promoting the apoptosis of MDA-MB-231 cells. Reckoning the findings of the study, SchA is a potential adjuvant treatment for breast cancer.
format	article
author	Ling Chen Li-Quan Ren Zhong Liu Xin Liu Han Tu Xu-Ying Huang
author_facet	Ling Chen Li-Quan Ren Zhong Liu Xin Liu Han Tu Xu-Ying Huang
author_sort	Ling Chen
title	Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells
title_short	Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells
title_full	Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells
title_fullStr	Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells
title_full_unstemmed	Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells
title_sort	bio-informatics and in vitro experiments reveal the mechanism of schisandrin a against mda-mb-231 cells
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/80813fd0c30c4706b7efad5586a578c6
work_keys_str_mv	AT lingchen bioinformaticsandinvitroexperimentsrevealthemechanismofschisandrinaagainstmdamb231cells AT liquanren bioinformaticsandinvitroexperimentsrevealthemechanismofschisandrinaagainstmdamb231cells AT zhongliu bioinformaticsandinvitroexperimentsrevealthemechanismofschisandrinaagainstmdamb231cells AT xinliu bioinformaticsandinvitroexperimentsrevealthemechanismofschisandrinaagainstmdamb231cells AT hantu bioinformaticsandinvitroexperimentsrevealthemechanismofschisandrinaagainstmdamb231cells AT xuyinghuang bioinformaticsandinvitroexperimentsrevealthemechanismofschisandrinaagainstmdamb231cells
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Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells

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