Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.

The outcome of many infections depends on the initial interactions between agent and host. Aiming at elucidating the effect of the M. tuberculosis Mce1 protein complex on host transcriptional and immunological responses to infection with M. tuberculosis, RNA from murine macrophages at 15, 30, 60 min...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ruth Stavrum, Anne-Kristin Stavrum, Håvard Valvatne, Lee W Riley, Elling Ulvestad, Inge Jonassen, Jörg Assmus, T Mark Doherty, Harleen M S Grewal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80895a8479bf4a1daf90a098e2e50040
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:80895a8479bf4a1daf90a098e2e50040
record_format	dspace
spelling	oai:doaj.org-article:80895a8479bf4a1daf90a098e2e500402021-11-18T07:35:50ZModulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.1932-620310.1371/journal.pone.0026295https://doaj.org/article/80895a8479bf4a1daf90a098e2e500402011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039457/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The outcome of many infections depends on the initial interactions between agent and host. Aiming at elucidating the effect of the M. tuberculosis Mce1 protein complex on host transcriptional and immunological responses to infection with M. tuberculosis, RNA from murine macrophages at 15, 30, 60 min, 4 and 10 hrs post-infection with M. tuberculosis H37Rv or Δ-mce1 H37Rv was analyzed by whole-genome microarrays and RT-QPCR. Immunological responses were measured using a 23-plex cytokine assay. Compared to uninfected controls, 524 versus 64 genes were up-regulated by 15 min post H37Rv- and Δ-mce1 H37Rv-infection, respectively. By 15 min post-H37Rv infection, a decline of 17 cytokines combined with up-regulation of Ccl24 (26.5-fold), Clec4a2 (23.2-fold) and Pparγ (10.5-fold) indicated an anti-inflammatory response initiated by IL-13. Down-regulation of Il13ra1 combined with up-regulation of Il12b (30.2-fold), suggested switch to a pro-inflammatory response by 4 hrs post H37Rv-infection. Whereas no significant change in cytokine concentration or transcription was observed during the first hour post Δ-mce1 H37Rv-infection, a significant decline of IL-1b, IL-9, IL-13, Eotaxin and GM-CSF combined with increased transcription of Il12b (25.1-fold) and Inb1 (17.9-fold) by 4 hrs, indicated a pro-inflammatory response. The balance between pro-and anti-inflammatory responses during the early stages of infection may have significant bearing on outcome.Ruth StavrumAnne-Kristin StavrumHåvard ValvatneLee W RileyElling UlvestadInge JonassenJörg AssmusT Mark DohertyHarleen M S GrewalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26295 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruth Stavrum Anne-Kristin Stavrum Håvard Valvatne Lee W Riley Elling Ulvestad Inge Jonassen Jörg Assmus T Mark Doherty Harleen M S Grewal Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.
description	The outcome of many infections depends on the initial interactions between agent and host. Aiming at elucidating the effect of the M. tuberculosis Mce1 protein complex on host transcriptional and immunological responses to infection with M. tuberculosis, RNA from murine macrophages at 15, 30, 60 min, 4 and 10 hrs post-infection with M. tuberculosis H37Rv or Δ-mce1 H37Rv was analyzed by whole-genome microarrays and RT-QPCR. Immunological responses were measured using a 23-plex cytokine assay. Compared to uninfected controls, 524 versus 64 genes were up-regulated by 15 min post H37Rv- and Δ-mce1 H37Rv-infection, respectively. By 15 min post-H37Rv infection, a decline of 17 cytokines combined with up-regulation of Ccl24 (26.5-fold), Clec4a2 (23.2-fold) and Pparγ (10.5-fold) indicated an anti-inflammatory response initiated by IL-13. Down-regulation of Il13ra1 combined with up-regulation of Il12b (30.2-fold), suggested switch to a pro-inflammatory response by 4 hrs post H37Rv-infection. Whereas no significant change in cytokine concentration or transcription was observed during the first hour post Δ-mce1 H37Rv-infection, a significant decline of IL-1b, IL-9, IL-13, Eotaxin and GM-CSF combined with increased transcription of Il12b (25.1-fold) and Inb1 (17.9-fold) by 4 hrs, indicated a pro-inflammatory response. The balance between pro-and anti-inflammatory responses during the early stages of infection may have significant bearing on outcome.
format	article
author	Ruth Stavrum Anne-Kristin Stavrum Håvard Valvatne Lee W Riley Elling Ulvestad Inge Jonassen Jörg Assmus T Mark Doherty Harleen M S Grewal
author_facet	Ruth Stavrum Anne-Kristin Stavrum Håvard Valvatne Lee W Riley Elling Ulvestad Inge Jonassen Jörg Assmus T Mark Doherty Harleen M S Grewal
author_sort	Ruth Stavrum
title	Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.
title_short	Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.
title_full	Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.
title_fullStr	Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.
title_full_unstemmed	Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.
title_sort	modulation of transcriptional and inflammatory responses in murine macrophages by the mycobacterium tuberculosis mammalian cell entry (mce) 1 complex.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/80895a8479bf4a1daf90a098e2e50040
work_keys_str_mv	AT ruthstavrum modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT annekristinstavrum modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT havardvalvatne modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT leewriley modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT ellingulvestad modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT ingejonassen modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT jorgassmus modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT tmarkdoherty modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex AT harleenmsgrewal modulationoftranscriptionalandinflammatoryresponsesinmurinemacrophagesbythemycobacteriumtuberculosismammaliancellentrymce1complex
_version_	1718423232721190912

Modulation of transcriptional and inflammatory responses in murine macrophages by the Mycobacterium tuberculosis mammalian cell entry (Mce) 1 complex.

Ejemplares similares