A robust in vitro model for trans-lymphatic endothelial migration

Abstract Trans-endothelial migration (TEM) is essential for leukocyte circulation. While much is known about trans-blood endothelial migration, far less is known about trans-lymphatic endothelial migration. We established an in vitro system to evaluate lymphatic TEM for various cell types across pri...

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Autores principales:	Yanbao Xiong, C. Colin Brinkman, Konrad S Famulski, Emmanuel F. Mongodin, Colin J. Lord, Keli L. Hippen, Bruce R. Blazar, Jonathan S. Bromberg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80899e2ada1c4615aa4c1a37324ff037
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spelling	oai:doaj.org-article:80899e2ada1c4615aa4c1a37324ff0372021-12-02T15:05:00ZA robust in vitro model for trans-lymphatic endothelial migration10.1038/s41598-017-01575-w2045-2322https://doaj.org/article/80899e2ada1c4615aa4c1a37324ff0372017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01575-whttps://doaj.org/toc/2045-2322Abstract Trans-endothelial migration (TEM) is essential for leukocyte circulation. While much is known about trans-blood endothelial migration, far less is known about trans-lymphatic endothelial migration. We established an in vitro system to evaluate lymphatic TEM for various cell types across primary mouse and human lymphatic endothelial cells (LEC), and validated the model for the murine LEC cell line SVEC4-10. T cells exhibited enhanced unidirectional migration from the basal (abluminal) to the apical (luminal) surface across LEC, whereas for blood endothelial cells (BEC) they migrated similarly in both directions. This preferential, vectorial migration was chemotactic toward many different chemoattractants and dose-dependent. Stromal protein fibers, interstitial type fluid flow, distribution of chemokines in the stromal layer, and inflammatory cytokines influenced LEC phenotype and leukocyte TEM. Activated and memory CD4 T cells, macrophages, and dendritic cell (DC) showed chemoattractantΔdriven vectorial migration, while CD8 T cell migration across LEC was not. The system was further validated for studying cancer cell transmigration across lymphatic endothelium. This model for lymphatic TEM for various migrating and endothelial cell types possesses the capacity to be high-throughput, highly reproducible and integrate the complexities of lymphatic biology, stromal variability, chemoattractant distribution, and fluid flow.Yanbao XiongC. Colin BrinkmanKonrad S FamulskiEmmanuel F. MongodinColin J. LordKeli L. HippenBruce R. BlazarJonathan S. BrombergNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanbao Xiong C. Colin Brinkman Konrad S Famulski Emmanuel F. Mongodin Colin J. Lord Keli L. Hippen Bruce R. Blazar Jonathan S. Bromberg A robust in vitro model for trans-lymphatic endothelial migration
description	Abstract Trans-endothelial migration (TEM) is essential for leukocyte circulation. While much is known about trans-blood endothelial migration, far less is known about trans-lymphatic endothelial migration. We established an in vitro system to evaluate lymphatic TEM for various cell types across primary mouse and human lymphatic endothelial cells (LEC), and validated the model for the murine LEC cell line SVEC4-10. T cells exhibited enhanced unidirectional migration from the basal (abluminal) to the apical (luminal) surface across LEC, whereas for blood endothelial cells (BEC) they migrated similarly in both directions. This preferential, vectorial migration was chemotactic toward many different chemoattractants and dose-dependent. Stromal protein fibers, interstitial type fluid flow, distribution of chemokines in the stromal layer, and inflammatory cytokines influenced LEC phenotype and leukocyte TEM. Activated and memory CD4 T cells, macrophages, and dendritic cell (DC) showed chemoattractantΔdriven vectorial migration, while CD8 T cell migration across LEC was not. The system was further validated for studying cancer cell transmigration across lymphatic endothelium. This model for lymphatic TEM for various migrating and endothelial cell types possesses the capacity to be high-throughput, highly reproducible and integrate the complexities of lymphatic biology, stromal variability, chemoattractant distribution, and fluid flow.
format	article
author	Yanbao Xiong C. Colin Brinkman Konrad S Famulski Emmanuel F. Mongodin Colin J. Lord Keli L. Hippen Bruce R. Blazar Jonathan S. Bromberg
author_facet	Yanbao Xiong C. Colin Brinkman Konrad S Famulski Emmanuel F. Mongodin Colin J. Lord Keli L. Hippen Bruce R. Blazar Jonathan S. Bromberg
author_sort	Yanbao Xiong
title	A robust in vitro model for trans-lymphatic endothelial migration
title_short	A robust in vitro model for trans-lymphatic endothelial migration
title_full	A robust in vitro model for trans-lymphatic endothelial migration
title_fullStr	A robust in vitro model for trans-lymphatic endothelial migration
title_full_unstemmed	A robust in vitro model for trans-lymphatic endothelial migration
title_sort	robust in vitro model for trans-lymphatic endothelial migration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/80899e2ada1c4615aa4c1a37324ff037
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_version_	1718388965389631488

A robust in vitro model for trans-lymphatic endothelial migration

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