Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.

<h4>Objective</h4>To assess the use of fourth-generation rapid diagnostic tests in identifying acute infection of Human Immunodeficiency Virus (HIV).<h4>Methods</h4>BCN Checkpoint promotes sexual health among men who have sex with men (MSM), with a focus on diagnosing HIV ear...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jorge Saz, Albert Dalmau-Bueno, Michael Meulbroek, Ferran Pujol, Josep Coll, Ángel Herraiz-Tomey, Félix Pérez, Giovanni Marazzi, Héctor Taboada, Dante R Culqui, Joan A Caylà, BCN Checkpoint Working Group
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8092037dbd154c13b18bedfe92165b2e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8092037dbd154c13b18bedfe92165b2e
record_format	dspace
spelling	oai:doaj.org-article:8092037dbd154c13b18bedfe92165b2e2021-12-02T20:06:22ZUse of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.1932-620310.1371/journal.pone.0255065https://doaj.org/article/8092037dbd154c13b18bedfe92165b2e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255065https://doaj.org/toc/1932-6203<h4>Objective</h4>To assess the use of fourth-generation rapid diagnostic tests in identifying acute infection of Human Immunodeficiency Virus (HIV).<h4>Methods</h4>BCN Checkpoint promotes sexual health among men who have sex with men (MSM), with a focus on diagnosing HIV early, initiating combined antiretroviral treatment (cART) promptly, and recommending regular repeat testing for those who have tested negative. This cross-sectional study included all test results obtained at the centre between 25 March 2016 and 24 March 2019. The Alere™ HIV Combo (now rebranded to Determine™ HIV Ultra, from Abbott) was used to detect p24 antigen (p24 Ag) and/or immunoglobulin M (IgM) and G (IgG) antibodies to HIV-1/HIV-2 (HIV Ab). Rapid polymerase chain reaction (PCR) confirmatory testing and Western blot (WB) were performed for clients with a positive rapid test result. Confirmed HIV cases were promptly referred to the HIV unit for care and cART prescription.<h4>Results</h4>A total of 12,961 clients attended BCN Checkpoint during the study and 27,298 rapid tests were performed. 450 tests were found to be reactive, of which 430 confirmed as HIV-positive, representing a prevalence of 3.32%. Four confirmed cases (0.93%) were detected as "p24 Ag only", nine (2.09%) as "both p24 and HIV Ab" and 417 (96.98%) as "HIV Ab only". The "p24 Ag only" group had a 1-log higher viral load than the other groups and initiated treatment on the following working day. Overall, there were 20 false-positive results (0.07% and 4.44% of total and reactive tests, respectively), of which 10 positive for "p24 Ag only" and 10 for "HIV Ab only".<h4>Conclusions</h4>Four Acute HIV Infections (AHI), with very high viral loads, have been detected with the "p24 Ag only" while the HIV Ab were still absent. Referral to the HIV unit and initiation of cART on the following working day contributed to improving persons' health and to reduce HIV transmission chain.Jorge SazAlbert Dalmau-BuenoMichael MeulbroekFerran PujolJosep CollÁngel Herraiz-TomeyFélix PérezGiovanni MarazziHéctor TaboadaDante R CulquiJoan A CaylàBCN Checkpoint Working GroupPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255065 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jorge Saz Albert Dalmau-Bueno Michael Meulbroek Ferran Pujol Josep Coll Ángel Herraiz-Tomey Félix Pérez Giovanni Marazzi Héctor Taboada Dante R Culqui Joan A Caylà BCN Checkpoint Working Group Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.
description	<h4>Objective</h4>To assess the use of fourth-generation rapid diagnostic tests in identifying acute infection of Human Immunodeficiency Virus (HIV).<h4>Methods</h4>BCN Checkpoint promotes sexual health among men who have sex with men (MSM), with a focus on diagnosing HIV early, initiating combined antiretroviral treatment (cART) promptly, and recommending regular repeat testing for those who have tested negative. This cross-sectional study included all test results obtained at the centre between 25 March 2016 and 24 March 2019. The Alere™ HIV Combo (now rebranded to Determine™ HIV Ultra, from Abbott) was used to detect p24 antigen (p24 Ag) and/or immunoglobulin M (IgM) and G (IgG) antibodies to HIV-1/HIV-2 (HIV Ab). Rapid polymerase chain reaction (PCR) confirmatory testing and Western blot (WB) were performed for clients with a positive rapid test result. Confirmed HIV cases were promptly referred to the HIV unit for care and cART prescription.<h4>Results</h4>A total of 12,961 clients attended BCN Checkpoint during the study and 27,298 rapid tests were performed. 450 tests were found to be reactive, of which 430 confirmed as HIV-positive, representing a prevalence of 3.32%. Four confirmed cases (0.93%) were detected as "p24 Ag only", nine (2.09%) as "both p24 and HIV Ab" and 417 (96.98%) as "HIV Ab only". The "p24 Ag only" group had a 1-log higher viral load than the other groups and initiated treatment on the following working day. Overall, there were 20 false-positive results (0.07% and 4.44% of total and reactive tests, respectively), of which 10 positive for "p24 Ag only" and 10 for "HIV Ab only".<h4>Conclusions</h4>Four Acute HIV Infections (AHI), with very high viral loads, have been detected with the "p24 Ag only" while the HIV Ab were still absent. Referral to the HIV unit and initiation of cART on the following working day contributed to improving persons' health and to reduce HIV transmission chain.
format	article
author	Jorge Saz Albert Dalmau-Bueno Michael Meulbroek Ferran Pujol Josep Coll Ángel Herraiz-Tomey Félix Pérez Giovanni Marazzi Héctor Taboada Dante R Culqui Joan A Caylà BCN Checkpoint Working Group
author_facet	Jorge Saz Albert Dalmau-Bueno Michael Meulbroek Ferran Pujol Josep Coll Ángel Herraiz-Tomey Félix Pérez Giovanni Marazzi Héctor Taboada Dante R Culqui Joan A Caylà BCN Checkpoint Working Group
author_sort	Jorge Saz
title	Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.
title_short	Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.
title_full	Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.
title_fullStr	Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.
title_full_unstemmed	Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.
title_sort	use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute hiv infection in a community centre for men who have sex with men, between 2016 and 2019.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8092037dbd154c13b18bedfe92165b2e
work_keys_str_mv	AT jorgesaz useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT albertdalmaubueno useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT michaelmeulbroek useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT ferranpujol useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT josepcoll useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT angelherraiztomey useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT felixperez useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT giovannimarazzi useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT hectortaboada useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT danterculqui useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT joanacayla useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019 AT bcncheckpointworkinggroup useoffourthgenerationrapidcombinedantigenandantibodydiagnostictestsforthedetectionofacutehivinfectioninacommunitycentreformenwhohavesexwithmenbetween2016and2019
_version_	1718375368819212288

Use of fourth-generation rapid combined antigen and antibody diagnostic tests for the detection of acute HIV infection in a community centre for men who have sex with men, between 2016 and 2019.

Ejemplares similares