Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

Abstract A large number of insect-specific viruses (ISVs) have recently been discovered, mostly from hematophagous insect vectors because of their medical importance, but little attention has been paid to important plant virus vectors such as the whitefly Bemisia tabaci, which exists as a complex of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hai-Jian Huang, Zhuang-Xin Ye, Xin Wang, Xiao-Tian Yan, Yan Zhang, Yu-Juan He, Yu-Hua Qi, Xiao-Di Zhang, Ji-Chong Zhuo, Gang Lu, Jia-Bao Lu, Qian-Zhuo Mao, Zong-Tao Sun, Fei Yan, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/80926bddc68146b884eb23b2e67e9e69
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:80926bddc68146b884eb23b2e67e9e69
record_format	dspace
spelling	oai:doaj.org-article:80926bddc68146b884eb23b2e67e9e692021-12-02T15:55:18ZDiversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci10.1038/s41522-021-00216-52055-5008https://doaj.org/article/80926bddc68146b884eb23b2e67e9e692021-05-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00216-5https://doaj.org/toc/2055-5008Abstract A large number of insect-specific viruses (ISVs) have recently been discovered, mostly from hematophagous insect vectors because of their medical importance, but little attention has been paid to important plant virus vectors such as the whitefly Bemisia tabaci, which exists as a complex of cryptic species. Public SRA datasets of B. tabaci and newly generated transcriptomes of three Chinese populations are here comprehensively investigated to characterize the whitefly viromes of different cryptic species. Twenty novel ISVs were confidently identified, mostly associated with a particular cryptic species while different cryptic species harbored one or more core ISVs. Microinjection experiments showed that some ISVs might cross-infect between the two invasive whitefly cryptic species, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), but others appeared to have a more restricted host range, reflecting the possibility of distinct long-term coevolution of these ISVs and whitefly hosts. Moreover, analysis of the profiles of virus-derived small-interfering RNAs indicated that some of the ISVs can successfully replicate in whitefly and the antiviral RNAi pathway of B. tabaci is actively involved in response to ISV infections. Our study provides a comprehensive analysis of the RNA virome, the distinct relationships and cross-cryptic species infectivity of ISVs in an agriculturally important insect vector.Hai-Jian HuangZhuang-Xin YeXin WangXiao-Tian YanYan ZhangYu-Juan HeYu-Hua QiXiao-Di ZhangJi-Chong ZhuoGang LuJia-Bao LuQian-Zhuo MaoZong-Tao SunFei YanJian-Ping ChenChuan-Xi ZhangJun-Min LiNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Hai-Jian Huang Zhuang-Xin Ye Xin Wang Xiao-Tian Yan Yan Zhang Yu-Juan He Yu-Hua Qi Xiao-Di Zhang Ji-Chong Zhuo Gang Lu Jia-Bao Lu Qian-Zhuo Mao Zong-Tao Sun Fei Yan Jian-Ping Chen Chuan-Xi Zhang Jun-Min Li Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
description	Abstract A large number of insect-specific viruses (ISVs) have recently been discovered, mostly from hematophagous insect vectors because of their medical importance, but little attention has been paid to important plant virus vectors such as the whitefly Bemisia tabaci, which exists as a complex of cryptic species. Public SRA datasets of B. tabaci and newly generated transcriptomes of three Chinese populations are here comprehensively investigated to characterize the whitefly viromes of different cryptic species. Twenty novel ISVs were confidently identified, mostly associated with a particular cryptic species while different cryptic species harbored one or more core ISVs. Microinjection experiments showed that some ISVs might cross-infect between the two invasive whitefly cryptic species, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), but others appeared to have a more restricted host range, reflecting the possibility of distinct long-term coevolution of these ISVs and whitefly hosts. Moreover, analysis of the profiles of virus-derived small-interfering RNAs indicated that some of the ISVs can successfully replicate in whitefly and the antiviral RNAi pathway of B. tabaci is actively involved in response to ISV infections. Our study provides a comprehensive analysis of the RNA virome, the distinct relationships and cross-cryptic species infectivity of ISVs in an agriculturally important insect vector.
format	article
author	Hai-Jian Huang Zhuang-Xin Ye Xin Wang Xiao-Tian Yan Yan Zhang Yu-Juan He Yu-Hua Qi Xiao-Di Zhang Ji-Chong Zhuo Gang Lu Jia-Bao Lu Qian-Zhuo Mao Zong-Tao Sun Fei Yan Jian-Ping Chen Chuan-Xi Zhang Jun-Min Li
author_facet	Hai-Jian Huang Zhuang-Xin Ye Xin Wang Xiao-Tian Yan Yan Zhang Yu-Juan He Yu-Hua Qi Xiao-Di Zhang Ji-Chong Zhuo Gang Lu Jia-Bao Lu Qian-Zhuo Mao Zong-Tao Sun Fei Yan Jian-Ping Chen Chuan-Xi Zhang Jun-Min Li
author_sort	Hai-Jian Huang
title	Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_short	Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_full	Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_fullStr	Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_full_unstemmed	Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci
title_sort	diversity and infectivity of the rna virome among different cryptic species of an agriculturally important insect vector: whitefly bemisia tabaci
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/80926bddc68146b884eb23b2e67e9e69
work_keys_str_mv	AT haijianhuang diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT zhuangxinye diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT xinwang diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT xiaotianyan diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT yanzhang diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT yujuanhe diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT yuhuaqi diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT xiaodizhang diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT jichongzhuo diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT ganglu diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT jiabaolu diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT qianzhuomao diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT zongtaosun diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT feiyan diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT jianpingchen diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT chuanxizhang diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci AT junminli diversityandinfectivityofthernaviromeamongdifferentcrypticspeciesofanagriculturallyimportantinsectvectorwhiteflybemisiatabaci
_version_	1718385399931338752

Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

Ejemplares similares