The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death

Abstract This work highlights how our silver ultra nanoclusters (ARGIRIUM-SUNc) hand-made synthesized, are very useful as a bactericide and anti-biofilm agent. The Argirium-SUNc effective antibacterial concentrations are very low (< 1 ppm) as compared to the corresponding values reported in the l...

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Autores principales:	Junior Bernardo Molina-Hernandez, Antonio Aceto, Tonino Bucciarelli, Domenico Paludi, Luca Valbonetti, Katiuscia Zilli, Luca Scotti, Clemencia Chaves-López
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80950618f232480bae1e158697c31ad0
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spelling	oai:doaj.org-article:80950618f232480bae1e158697c31ad02021-11-08T10:50:39ZThe membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death10.1038/s41598-021-00545-72045-2322https://doaj.org/article/80950618f232480bae1e158697c31ad02021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00545-7https://doaj.org/toc/2045-2322Abstract This work highlights how our silver ultra nanoclusters (ARGIRIUM-SUNc) hand-made synthesized, are very useful as a bactericide and anti-biofilm agent. The Argirium-SUNc effective antibacterial concentrations are very low (< 1 ppm) as compared to the corresponding values reported in the literature. Different bacterial defense mechanisms are observed dependent on ARGIRIUM-SUNc concentrations. Biochemical investigations (volatilome) have been performed to understand the pathways involved in cell death. By using fluorescence techniques and cell viability measurements we show, for the first time, that membrane depolarization and calcium intracellular level are both primary events in bacteria death. The ARGIRIUM-SUNc determined eradication of different biofilm at a concentration as low as 0.6 ppm. This suggests that the effect of the nanoparticles follows a common mechanism in different bacteria. It is highly probable that the chemical constitution of the crosslinks could be a key target in the disrupting mechanism of our nanoparticles. Since the biofilms and their constituents are essential for bacterial survival in contact with humans, the silver nanoparticles represent a logical target for new antibacterial treatments.Junior Bernardo Molina-HernandezAntonio AcetoTonino BucciarelliDomenico PaludiLuca ValbonettiKatiuscia ZilliLuca ScottiClemencia Chaves-LópezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Junior Bernardo Molina-Hernandez Antonio Aceto Tonino Bucciarelli Domenico Paludi Luca Valbonetti Katiuscia Zilli Luca Scotti Clemencia Chaves-López The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death
description	Abstract This work highlights how our silver ultra nanoclusters (ARGIRIUM-SUNc) hand-made synthesized, are very useful as a bactericide and anti-biofilm agent. The Argirium-SUNc effective antibacterial concentrations are very low (< 1 ppm) as compared to the corresponding values reported in the literature. Different bacterial defense mechanisms are observed dependent on ARGIRIUM-SUNc concentrations. Biochemical investigations (volatilome) have been performed to understand the pathways involved in cell death. By using fluorescence techniques and cell viability measurements we show, for the first time, that membrane depolarization and calcium intracellular level are both primary events in bacteria death. The ARGIRIUM-SUNc determined eradication of different biofilm at a concentration as low as 0.6 ppm. This suggests that the effect of the nanoparticles follows a common mechanism in different bacteria. It is highly probable that the chemical constitution of the crosslinks could be a key target in the disrupting mechanism of our nanoparticles. Since the biofilms and their constituents are essential for bacterial survival in contact with humans, the silver nanoparticles represent a logical target for new antibacterial treatments.
format	article
author	Junior Bernardo Molina-Hernandez Antonio Aceto Tonino Bucciarelli Domenico Paludi Luca Valbonetti Katiuscia Zilli Luca Scotti Clemencia Chaves-López
author_facet	Junior Bernardo Molina-Hernandez Antonio Aceto Tonino Bucciarelli Domenico Paludi Luca Valbonetti Katiuscia Zilli Luca Scotti Clemencia Chaves-López
author_sort	Junior Bernardo Molina-Hernandez
title	The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death
title_short	The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death
title_full	The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death
title_fullStr	The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death
title_full_unstemmed	The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death
title_sort	membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/80950618f232480bae1e158697c31ad0
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The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death

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