Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador

Abstract. Sarmiento-Tomalá G, Santos-Ordóñez E, Miranda-Martínez M, Pacheco-Coello R, Scull-Lizama R, Gutiérrez-Gaitén Y, Delgado-Hernández R. 2020. Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L from Ecuador. Biodiver...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	GLENDA SARMIENTO-TOMALÁ, Efrén Santos-Ordóñez, MIGDALIA MIRANDA-MARTÍNEZ, RICARDO PACHECO-COELLO, RAMÓN SCULL-LIZAMA, YAMILET GUTIÉRREZ-GAITÉN, RENÉ RENÉ DELGADO-HERNÁNDEZ
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	internal transcribed spacer its1 its2 matk micromorphology molecular markers rbcl Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/809aa923e76f4e7bad73531557fdfdd7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:809aa923e76f4e7bad73531557fdfdd7
record_format	dspace
spelling	oai:doaj.org-article:809aa923e76f4e7bad73531557fdfdd72021-11-22T12:17:57ZShort Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador1412-033X2085-472210.13057/biodiv/d210818https://doaj.org/article/809aa923e76f4e7bad73531557fdfdd72020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6204https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sarmiento-Tomalá G, Santos-Ordóñez E, Miranda-Martínez M, Pacheco-Coello R, Scull-Lizama R, Gutiérrez-Gaitén Y, Delgado-Hernández R. 2020. Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L from Ecuador. Biodiversitas 21: 3554-3560. In Ecuador, several plant species are used in traditional medicine without a criterion of family, genera, or chemical composition. The species of the genus Malva (Malva pseudolavatera Webb & Berthel and Malva sylvestris L), introduced in Ecuador, are widely used by the population; however, unlike the species M. sylvestris, for M. pseudolavatera there is no information about its composition and properties. Plant material was collected in the province of Chimborazo in Ecuador and taxonomic classification was performed. Histological study was performed in leaves and powder drug. Molecular barcodes were generated using the ribulose bisphosphate carboxylase large chain (rbcL), maturase K (matK), internal transcribed spacer 1 (ITS1) and ITS2 sequences. Micro-morphological analysis revealed that no major structural differences were observed between the two species. Sequence analysis of molecular barcodes revealed that samples of the different species showed a close relation to each other due to the high percentage of similarity. The ITS sequences showed that the two samples correspond to different species of Malva; while for the rbcL and matK, interspecies differentiation could not be detected. Therefore, ITS could be used for interspecific analysis.GLENDA SARMIENTO-TOMALÁEfrén Santos-OrdóñezMIGDALIA MIRANDA-MARTÍNEZRICARDO PACHECO-COELLORAMÓN SCULL-LIZAMAYAMILET GUTIÉRREZ-GAITÉNRENÉ RENÉ DELGADO-HERNÁNDEZMBI & UNS Soloarticleinternal transcribed spacerits1its2matkmicromorphologymolecular markersrbclBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	internal transcribed spacer its1 its2 matk micromorphology molecular markers rbcl Biology (General) QH301-705.5
spellingShingle	internal transcribed spacer its1 its2 matk micromorphology molecular markers rbcl Biology (General) QH301-705.5 GLENDA SARMIENTO-TOMALÁ Efrén Santos-Ordóñez MIGDALIA MIRANDA-MARTÍNEZ RICARDO PACHECO-COELLO RAMÓN SCULL-LIZAMA YAMILET GUTIÉRREZ-GAITÉN RENÉ RENÉ DELGADO-HERNÁNDEZ Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador
description	Abstract. Sarmiento-Tomalá G, Santos-Ordóñez E, Miranda-Martínez M, Pacheco-Coello R, Scull-Lizama R, Gutiérrez-Gaitén Y, Delgado-Hernández R. 2020. Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L from Ecuador. Biodiversitas 21: 3554-3560. In Ecuador, several plant species are used in traditional medicine without a criterion of family, genera, or chemical composition. The species of the genus Malva (Malva pseudolavatera Webb & Berthel and Malva sylvestris L), introduced in Ecuador, are widely used by the population; however, unlike the species M. sylvestris, for M. pseudolavatera there is no information about its composition and properties. Plant material was collected in the province of Chimborazo in Ecuador and taxonomic classification was performed. Histological study was performed in leaves and powder drug. Molecular barcodes were generated using the ribulose bisphosphate carboxylase large chain (rbcL), maturase K (matK), internal transcribed spacer 1 (ITS1) and ITS2 sequences. Micro-morphological analysis revealed that no major structural differences were observed between the two species. Sequence analysis of molecular barcodes revealed that samples of the different species showed a close relation to each other due to the high percentage of similarity. The ITS sequences showed that the two samples correspond to different species of Malva; while for the rbcL and matK, interspecies differentiation could not be detected. Therefore, ITS could be used for interspecific analysis.
format	article
author	GLENDA SARMIENTO-TOMALÁ Efrén Santos-Ordóñez MIGDALIA MIRANDA-MARTÍNEZ RICARDO PACHECO-COELLO RAMÓN SCULL-LIZAMA YAMILET GUTIÉRREZ-GAITÉN RENÉ RENÉ DELGADO-HERNÁNDEZ
author_facet	GLENDA SARMIENTO-TOMALÁ Efrén Santos-Ordóñez MIGDALIA MIRANDA-MARTÍNEZ RICARDO PACHECO-COELLO RAMÓN SCULL-LIZAMA YAMILET GUTIÉRREZ-GAITÉN RENÉ RENÉ DELGADO-HERNÁNDEZ
author_sort	GLENDA SARMIENTO-TOMALÁ
title	Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador
title_short	Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador
title_full	Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador
title_fullStr	Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador
title_full_unstemmed	Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador
title_sort	short communication: molecular barcode and morphology analysis of malva pseudolavatera webb & berthel and malva sylvestris l. from ecuador
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/809aa923e76f4e7bad73531557fdfdd7
work_keys_str_mv	AT glendasarmientotomala shortcommunicationmolecularbarcodeandmorphologyanalysisofmalvapseudolavaterawebbberthelandmalvasylvestrislfromecuador AT efrensantosordonez shortcommunicationmolecularbarcodeandmorphologyanalysisofmalvapseudolavaterawebbberthelandmalvasylvestrislfromecuador AT migdaliamirandamartinez shortcommunicationmolecularbarcodeandmorphologyanalysisofmalvapseudolavaterawebbberthelandmalvasylvestrislfromecuador AT ricardopachecocoello shortcommunicationmolecularbarcodeandmorphologyanalysisofmalvapseudolavaterawebbberthelandmalvasylvestrislfromecuador AT ramonsculllizama shortcommunicationmolecularbarcodeandmorphologyanalysisofmalvapseudolavaterawebbberthelandmalvasylvestrislfromecuador AT yamiletgutierrezgaiten shortcommunicationmolecularbarcodeandmorphologyanalysisofmalvapseudolavaterawebbberthelandmalvasylvestrislfromecuador AT renerenedelgadohernandez shortcommunicationmolecularbarcodeandmorphologyanalysisofmalvapseudolavaterawebbberthelandmalvasylvestrislfromecuador
_version_	1718417620612415488

Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador

Ejemplares similares