High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.

Additional chromosomal abnormalities are currently detected in Burkitt's lymphoma. They play major roles in the progression of BL and in prognosis. The genes involved remain elusive. A whole-genome oligonucleotide array CGH analysis correlated with karyotype and FISH was performed in a set of 2...

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Autores principales:	Saloua Toujani, Philippe Dessen, Nathalie Ithzar, Gisèle Danglot, Catherine Richon, Yegor Vassetzky, Thomas Robert, Vladimir Lazar, Jacques Bosq, Lydie Da Costa, Christine Pérot, Vincent Ribrag, Catherine Patte, Jöelle Wiels, Alain Bernheim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/809e18ff1b0b415a95e53c9a21fbdb2a
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spelling	oai:doaj.org-article:809e18ff1b0b415a95e53c9a21fbdb2a2021-11-25T06:20:19ZHigh resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.1932-620310.1371/journal.pone.0007089https://doaj.org/article/809e18ff1b0b415a95e53c9a21fbdb2a2009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19759907/?tool=EBIhttps://doaj.org/toc/1932-6203Additional chromosomal abnormalities are currently detected in Burkitt's lymphoma. They play major roles in the progression of BL and in prognosis. The genes involved remain elusive. A whole-genome oligonucleotide array CGH analysis correlated with karyotype and FISH was performed in a set of 27 Burkitt's lymphoma-derived cell lines and primary tumors. More than half of the 145 CNAs<2 Mb were mapped to Mendelian CNVs, including GSTT1, glutathione s-transferase and BIRC6, an anti-apoptotic protein, possibly predisposing to some cancers. Somatic cell line-specific CNVs localized to the IG locus were consistently observed with the 244 K aCGH platform. Among 136 CNAs >2 Mb, gains were found in 1q (12/27), 13q (7/27), 7q (6/27), 8q(4/27), 2p (3/27), 11q (2/27) and 15q (2/27). Losses were found in 3p (5/27), 4p (4/27), 4q (4/27), 9p (4/27), 13q (4/27), 6p (3/27), 17p (3/27), 6q (2/27),11pterp13 (2/27) and 14q12q21.3 (2/27). Twenty one minimal critical regions (MCR), (range 0.04-71.36 Mb), were delineated in tumors and cell lines. Three MCRs were localized to 1q. The proximal one was mapped to 1q21.1q25.2 with a 6.3 Mb amplicon (1q21.1q21.3) harboring BCA2 and PIAS3. In the other 2 MCRs, 1q32.1 and 1q44, MDM4 and AKT3 appeared as possible drivers of these gains respectively. The 13q31.3q32.1 <89.58-96.81> MCR contained an amplicon and ABCC4 might be the driver of this amplicon. The 40 Kb 2p16.1 <60.96-61> MCR was the smallest gained MCR and specifically encompassed the REL oncogene which is already implicated in B cell lymphomas. The most frequently deleted MCR was 3p14.1 <60.43-60.53> that removed the fifth exon of FHIT. Further investigations which combined gene expression and functional studies are essential to understand the lymphomagenesis mechanism and for the development of more effective, targeted therapeutic strategies.Saloua ToujaniPhilippe DessenNathalie IthzarGisèle DanglotCatherine RichonYegor VassetzkyThomas RobertVladimir LazarJacques BosqLydie Da CostaChristine PérotVincent RibragCatherine PatteJöelle WielsAlain BernheimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 9, p e7089 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saloua Toujani Philippe Dessen Nathalie Ithzar Gisèle Danglot Catherine Richon Yegor Vassetzky Thomas Robert Vladimir Lazar Jacques Bosq Lydie Da Costa Christine Pérot Vincent Ribrag Catherine Patte Jöelle Wiels Alain Bernheim High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.
description	Additional chromosomal abnormalities are currently detected in Burkitt's lymphoma. They play major roles in the progression of BL and in prognosis. The genes involved remain elusive. A whole-genome oligonucleotide array CGH analysis correlated with karyotype and FISH was performed in a set of 27 Burkitt's lymphoma-derived cell lines and primary tumors. More than half of the 145 CNAs<2 Mb were mapped to Mendelian CNVs, including GSTT1, glutathione s-transferase and BIRC6, an anti-apoptotic protein, possibly predisposing to some cancers. Somatic cell line-specific CNVs localized to the IG locus were consistently observed with the 244 K aCGH platform. Among 136 CNAs >2 Mb, gains were found in 1q (12/27), 13q (7/27), 7q (6/27), 8q(4/27), 2p (3/27), 11q (2/27) and 15q (2/27). Losses were found in 3p (5/27), 4p (4/27), 4q (4/27), 9p (4/27), 13q (4/27), 6p (3/27), 17p (3/27), 6q (2/27),11pterp13 (2/27) and 14q12q21.3 (2/27). Twenty one minimal critical regions (MCR), (range 0.04-71.36 Mb), were delineated in tumors and cell lines. Three MCRs were localized to 1q. The proximal one was mapped to 1q21.1q25.2 with a 6.3 Mb amplicon (1q21.1q21.3) harboring BCA2 and PIAS3. In the other 2 MCRs, 1q32.1 and 1q44, MDM4 and AKT3 appeared as possible drivers of these gains respectively. The 13q31.3q32.1 <89.58-96.81> MCR contained an amplicon and ABCC4 might be the driver of this amplicon. The 40 Kb 2p16.1 <60.96-61> MCR was the smallest gained MCR and specifically encompassed the REL oncogene which is already implicated in B cell lymphomas. The most frequently deleted MCR was 3p14.1 <60.43-60.53> that removed the fifth exon of FHIT. Further investigations which combined gene expression and functional studies are essential to understand the lymphomagenesis mechanism and for the development of more effective, targeted therapeutic strategies.
format	article
author	Saloua Toujani Philippe Dessen Nathalie Ithzar Gisèle Danglot Catherine Richon Yegor Vassetzky Thomas Robert Vladimir Lazar Jacques Bosq Lydie Da Costa Christine Pérot Vincent Ribrag Catherine Patte Jöelle Wiels Alain Bernheim
author_facet	Saloua Toujani Philippe Dessen Nathalie Ithzar Gisèle Danglot Catherine Richon Yegor Vassetzky Thomas Robert Vladimir Lazar Jacques Bosq Lydie Da Costa Christine Pérot Vincent Ribrag Catherine Patte Jöelle Wiels Alain Bernheim
author_sort	Saloua Toujani
title	High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.
title_short	High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.
title_full	High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.
title_fullStr	High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.
title_full_unstemmed	High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.
title_sort	high resolution genome-wide analysis of chromosomal alterations in burkitt's lymphoma.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/809e18ff1b0b415a95e53c9a21fbdb2a
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High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.

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