Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response

Abstract Previous work using non-invasive radiofrequency field treatment (RFT) in cancer has demonstrated its therapeutic potential as it can increase intratumoral blood perfusion, localization of intravenously delivered drugs, and promote a hyperthermic intratumoral state. Despite the well-known im...

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Autores principales:	Jared M. Newton, Jose H. Flores-Arredondo, Sarah Suki, Matthew J. Ware, Martyna Krzykawska-Serda, Mahdi Agha, Justin J. Law, Andrew G. Sikora, Steven A. Curley, Stuart J. Corr
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/809ec0e77ba64c8fbfe3cba2d3bfacd3
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spelling	oai:doaj.org-article:809ec0e77ba64c8fbfe3cba2d3bfacd32021-12-02T16:08:15ZNon-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response10.1038/s41598-018-21719-w2045-2322https://doaj.org/article/809ec0e77ba64c8fbfe3cba2d3bfacd32018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21719-whttps://doaj.org/toc/2045-2322Abstract Previous work using non-invasive radiofrequency field treatment (RFT) in cancer has demonstrated its therapeutic potential as it can increase intratumoral blood perfusion, localization of intravenously delivered drugs, and promote a hyperthermic intratumoral state. Despite the well-known immunologic benefits that febrile hyperthermia can induce, an investigation of how RFT could modulate the intra-tumoral immune microenvironment had not been studied. Thus, using an established 4T1 breast cancer model in immune competent mice, we demonstrate that RFT induces a transient, localized, and T-cell dependent intratumoral inflammatory response. More specifically we show that multi- and singlet-dose RFT promote an increase in tumor volume in immune competent Balb/c mice, which does not occur in athymic nude models. Further leukocyte subset analysis at 24, 48, and 120 hours after a single RFT show a rapid increase in tumoral trafficking of CD4+ and CD8+ T-cells 24 hours post-treatment. Additional serum cytokine analysis reveals an increase in numerous pro-inflammatory cytokines and chemokines associated with enhanced T-cell trafficking. Overall, these data demonstrate that non-invasive RFT could be an effective immunomodulatory strategy in solid tumors, especially for enhancing the tumoral trafficking of lymphocytes, which is currently a major hindrance of numerous cancer immunotherapeutic strategies.Jared M. NewtonJose H. Flores-ArredondoSarah SukiMatthew J. WareMartyna Krzykawska-SerdaMahdi AghaJustin J. LawAndrew G. SikoraSteven A. CurleyStuart J. CorrNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jared M. Newton Jose H. Flores-Arredondo Sarah Suki Matthew J. Ware Martyna Krzykawska-Serda Mahdi Agha Justin J. Law Andrew G. Sikora Steven A. Curley Stuart J. Corr Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response
description	Abstract Previous work using non-invasive radiofrequency field treatment (RFT) in cancer has demonstrated its therapeutic potential as it can increase intratumoral blood perfusion, localization of intravenously delivered drugs, and promote a hyperthermic intratumoral state. Despite the well-known immunologic benefits that febrile hyperthermia can induce, an investigation of how RFT could modulate the intra-tumoral immune microenvironment had not been studied. Thus, using an established 4T1 breast cancer model in immune competent mice, we demonstrate that RFT induces a transient, localized, and T-cell dependent intratumoral inflammatory response. More specifically we show that multi- and singlet-dose RFT promote an increase in tumor volume in immune competent Balb/c mice, which does not occur in athymic nude models. Further leukocyte subset analysis at 24, 48, and 120 hours after a single RFT show a rapid increase in tumoral trafficking of CD4+ and CD8+ T-cells 24 hours post-treatment. Additional serum cytokine analysis reveals an increase in numerous pro-inflammatory cytokines and chemokines associated with enhanced T-cell trafficking. Overall, these data demonstrate that non-invasive RFT could be an effective immunomodulatory strategy in solid tumors, especially for enhancing the tumoral trafficking of lymphocytes, which is currently a major hindrance of numerous cancer immunotherapeutic strategies.
format	article
author	Jared M. Newton Jose H. Flores-Arredondo Sarah Suki Matthew J. Ware Martyna Krzykawska-Serda Mahdi Agha Justin J. Law Andrew G. Sikora Steven A. Curley Stuart J. Corr
author_facet	Jared M. Newton Jose H. Flores-Arredondo Sarah Suki Matthew J. Ware Martyna Krzykawska-Serda Mahdi Agha Justin J. Law Andrew G. Sikora Steven A. Curley Stuart J. Corr
author_sort	Jared M. Newton
title	Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response
title_short	Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response
title_full	Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response
title_fullStr	Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response
title_full_unstemmed	Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response
title_sort	non-invasive radiofrequency field treatment of 4t1 breast tumors induces t-cell dependent inflammatory response
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/809ec0e77ba64c8fbfe3cba2d3bfacd3
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Non-Invasive Radiofrequency Field Treatment of 4T1 Breast Tumors Induces T-cell Dependent Inflammatory Response

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