Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.

A PEG-based, folate mediated, active tumor targeting drug delivery system using DOX-hyd-PEG-FA nanoparticles (NPs) were prepared. DOX-hyd-PEG-FA NPs showed a significantly faster DOX release in pH 5.0 medium than in pH 7.4 medium. Compared with DOX-hyd-PEG NPs, DOX-hyd-PEG-FA NPs increased the intra...

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Autores principales:	Wei-liang Ye, Jiang-bo Du, Bang-le Zhang, Ren Na, Yan-feng Song, Qi-bing Mei, Ming-gao Zhao, Si-yuan Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80a4eb0980d54e93a5c8da5ddaa2dfef
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spelling	oai:doaj.org-article:80a4eb0980d54e93a5c8da5ddaa2dfef2021-11-18T08:19:16ZCellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.1932-620310.1371/journal.pone.0097358https://doaj.org/article/80a4eb0980d54e93a5c8da5ddaa2dfef2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24828815/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A PEG-based, folate mediated, active tumor targeting drug delivery system using DOX-hyd-PEG-FA nanoparticles (NPs) were prepared. DOX-hyd-PEG-FA NPs showed a significantly faster DOX release in pH 5.0 medium than in pH 7.4 medium. Compared with DOX-hyd-PEG NPs, DOX-hyd-PEG-FA NPs increased the intracellular accumulation of DOX and showed a DOX translocation from lysosomes to nucleus. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was much higher than that of free DOX, DOX-ami-PEG-FA NPs and DOX-hyd-PEG NPs. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was attenuated in the presence of exogenous folic acid. The IC50 of DOX-hyd-PEG-FA NPs and DOX-hyd-PEG NPs on A549 cells showed no significant difference. After DOX-hyd-PEG-FA NPs were intravenously administered, the amount of DOX distributed in tumor tissue was significantly increased, while the amount of DOX distributed in heart was greatly decreased as compared with free DOX. Compared with free DOX, NPs yielded improved survival rate, prolonged life span, delayed tumor growth and reduced the cardiotoxicity in tumor bearing mice model. These results indicated that the acid sensitivity, passive and active tumor targeting abilities were likely to act synergistically to enhance the drug delivery efficiency of DOX-hyd-PEG-FA NPs. Therefore, DOX-hyd-PEG-FA NPs are a promising drug delivery system for targeted cancer therapy.Wei-liang YeJiang-bo DuBang-le ZhangRen NaYan-feng SongQi-bing MeiMing-gao ZhaoSi-yuan ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e97358 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei-liang Ye Jiang-bo Du Bang-le Zhang Ren Na Yan-feng Song Qi-bing Mei Ming-gao Zhao Si-yuan Zhou Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.
description	A PEG-based, folate mediated, active tumor targeting drug delivery system using DOX-hyd-PEG-FA nanoparticles (NPs) were prepared. DOX-hyd-PEG-FA NPs showed a significantly faster DOX release in pH 5.0 medium than in pH 7.4 medium. Compared with DOX-hyd-PEG NPs, DOX-hyd-PEG-FA NPs increased the intracellular accumulation of DOX and showed a DOX translocation from lysosomes to nucleus. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was much higher than that of free DOX, DOX-ami-PEG-FA NPs and DOX-hyd-PEG NPs. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was attenuated in the presence of exogenous folic acid. The IC50 of DOX-hyd-PEG-FA NPs and DOX-hyd-PEG NPs on A549 cells showed no significant difference. After DOX-hyd-PEG-FA NPs were intravenously administered, the amount of DOX distributed in tumor tissue was significantly increased, while the amount of DOX distributed in heart was greatly decreased as compared with free DOX. Compared with free DOX, NPs yielded improved survival rate, prolonged life span, delayed tumor growth and reduced the cardiotoxicity in tumor bearing mice model. These results indicated that the acid sensitivity, passive and active tumor targeting abilities were likely to act synergistically to enhance the drug delivery efficiency of DOX-hyd-PEG-FA NPs. Therefore, DOX-hyd-PEG-FA NPs are a promising drug delivery system for targeted cancer therapy.
format	article
author	Wei-liang Ye Jiang-bo Du Bang-le Zhang Ren Na Yan-feng Song Qi-bing Mei Ming-gao Zhao Si-yuan Zhou
author_facet	Wei-liang Ye Jiang-bo Du Bang-le Zhang Ren Na Yan-feng Song Qi-bing Mei Ming-gao Zhao Si-yuan Zhou
author_sort	Wei-liang Ye
title	Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.
title_short	Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.
title_full	Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.
title_fullStr	Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.
title_full_unstemmed	Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.
title_sort	cellular uptake and antitumor activity of dox-hyd-peg-fa nanoparticles.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/80a4eb0980d54e93a5c8da5ddaa2dfef
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Cellular uptake and antitumor activity of DOX-hyd-PEG-FA nanoparticles.

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