Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus
Blackleg is one of the major fungal diseases in oilseed rape/canola worldwide. Most commercial cultivars carry R gene-mediated qualitative resistances that confer a high level of race-specific protection against Leptosphaeria maculans, the causal fungus of blackleg disease. However, monogenic resist...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:80aa4dc4aab24a5c90b7f86a261e40a52021-11-18T06:31:14ZDissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus1664-462X10.3389/fpls.2021.749491https://doaj.org/article/80aa4dc4aab24a5c90b7f86a261e40a52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.749491/fullhttps://doaj.org/toc/1664-462XBlackleg is one of the major fungal diseases in oilseed rape/canola worldwide. Most commercial cultivars carry R gene-mediated qualitative resistances that confer a high level of race-specific protection against Leptosphaeria maculans, the causal fungus of blackleg disease. However, monogenic resistances of this kind can potentially be rapidly overcome by mutations in the pathogen’s avirulence genes. To counteract pathogen adaptation in this evolutionary arms race, there is a tremendous demand for quantitative background resistance to enhance durability and efficacy of blackleg resistance in oilseed rape. In this study, we characterized genomic regions contributing to quantitative L. maculans resistance by genome-wide association studies in a multiparental mapping population derived from six parental elite varieties exhibiting quantitative resistance, which were all crossed to one common susceptible parental elite variety. Resistance was screened using a fungal isolate with no corresponding avirulence (AvrLm) to major R genes present in the parents of the mapping population. Genome-wide association studies revealed eight significantly associated quantitative trait loci (QTL) on chromosomes A07 and A09, with small effects explaining 3–6% of the phenotypic variance. Unexpectedly, the qualitative blackleg resistance gene Rlm9 was found to be located within a resistance-associated haploblock on chromosome A07. Furthermore, long-range sequence data spanning this haploblock revealed high levels of single-nucleotide and structural variants within the Rlm9 coding sequence among the parents of the mapping population. The results suggest that novel variants of Rlm9 could play a previously unknown role in expression of quantitative disease resistance in oilseed rape.Paul VollrathHarmeet S. ChawlaDima AlnajarIulian GaburIulian GaburHueyTyng LeeSven WeberLennard EhrigBirger KoopmannRod J. SnowdonChristian ObermeierFrontiers Media S.A.articleONTstructural variationblacklegBrassica napuslong-read sequencingRlm9Plant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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ONT structural variation blackleg Brassica napus long-read sequencing Rlm9 Plant culture SB1-1110 |
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ONT structural variation blackleg Brassica napus long-read sequencing Rlm9 Plant culture SB1-1110 Paul Vollrath Harmeet S. Chawla Dima Alnajar Iulian Gabur Iulian Gabur HueyTyng Lee Sven Weber Lennard Ehrig Birger Koopmann Rod J. Snowdon Christian Obermeier Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus |
| description |
Blackleg is one of the major fungal diseases in oilseed rape/canola worldwide. Most commercial cultivars carry R gene-mediated qualitative resistances that confer a high level of race-specific protection against Leptosphaeria maculans, the causal fungus of blackleg disease. However, monogenic resistances of this kind can potentially be rapidly overcome by mutations in the pathogen’s avirulence genes. To counteract pathogen adaptation in this evolutionary arms race, there is a tremendous demand for quantitative background resistance to enhance durability and efficacy of blackleg resistance in oilseed rape. In this study, we characterized genomic regions contributing to quantitative L. maculans resistance by genome-wide association studies in a multiparental mapping population derived from six parental elite varieties exhibiting quantitative resistance, which were all crossed to one common susceptible parental elite variety. Resistance was screened using a fungal isolate with no corresponding avirulence (AvrLm) to major R genes present in the parents of the mapping population. Genome-wide association studies revealed eight significantly associated quantitative trait loci (QTL) on chromosomes A07 and A09, with small effects explaining 3–6% of the phenotypic variance. Unexpectedly, the qualitative blackleg resistance gene Rlm9 was found to be located within a resistance-associated haploblock on chromosome A07. Furthermore, long-range sequence data spanning this haploblock revealed high levels of single-nucleotide and structural variants within the Rlm9 coding sequence among the parents of the mapping population. The results suggest that novel variants of Rlm9 could play a previously unknown role in expression of quantitative disease resistance in oilseed rape. |
| format |
article |
| author |
Paul Vollrath Harmeet S. Chawla Dima Alnajar Iulian Gabur Iulian Gabur HueyTyng Lee Sven Weber Lennard Ehrig Birger Koopmann Rod J. Snowdon Christian Obermeier |
| author_facet |
Paul Vollrath Harmeet S. Chawla Dima Alnajar Iulian Gabur Iulian Gabur HueyTyng Lee Sven Weber Lennard Ehrig Birger Koopmann Rod J. Snowdon Christian Obermeier |
| author_sort |
Paul Vollrath |
| title |
Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus |
| title_short |
Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus |
| title_full |
Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus |
| title_fullStr |
Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus |
| title_full_unstemmed |
Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance Gene Rlm9 in Elite Brassica napus |
| title_sort |
dissection of quantitative blackleg resistance reveals novel variants of resistance gene rlm9 in elite brassica napus |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/80aa4dc4aab24a5c90b7f86a261e40a5 |
| work_keys_str_mv |
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