Bracoviruses recruit host integrases for their integration into caterpillar's genome.

Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles i...

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Autores principales:	Zehua Wang, Xiqian Ye, Yuenan Zhou, Xiaotong Wu, Rongmin Hu, Jiachen Zhu, Ting Chen, Elisabeth Huguet, Min Shi, Jean-Michel Drezen, Jianhua Huang, Xuexin Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/80ac222e67bc44f1a608400f34952dc4
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spelling	oai:doaj.org-article:80ac222e67bc44f1a608400f34952dc42021-12-02T20:03:21ZBracoviruses recruit host integrases for their integration into caterpillar's genome.1553-73901553-740410.1371/journal.pgen.1009751https://doaj.org/article/80ac222e67bc44f1a608400f34952dc42021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009751https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential "8+5" nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar's genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts.Zehua WangXiqian YeYuenan ZhouXiaotong WuRongmin HuJiachen ZhuTing ChenElisabeth HuguetMin ShiJean-Michel DrezenJianhua HuangXuexin ChenPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009751 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Zehua Wang Xiqian Ye Yuenan Zhou Xiaotong Wu Rongmin Hu Jiachen Zhu Ting Chen Elisabeth Huguet Min Shi Jean-Michel Drezen Jianhua Huang Xuexin Chen Bracoviruses recruit host integrases for their integration into caterpillar's genome.
description	Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential "8+5" nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar's genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts.
format	article
author	Zehua Wang Xiqian Ye Yuenan Zhou Xiaotong Wu Rongmin Hu Jiachen Zhu Ting Chen Elisabeth Huguet Min Shi Jean-Michel Drezen Jianhua Huang Xuexin Chen
author_facet	Zehua Wang Xiqian Ye Yuenan Zhou Xiaotong Wu Rongmin Hu Jiachen Zhu Ting Chen Elisabeth Huguet Min Shi Jean-Michel Drezen Jianhua Huang Xuexin Chen
author_sort	Zehua Wang
title	Bracoviruses recruit host integrases for their integration into caterpillar's genome.
title_short	Bracoviruses recruit host integrases for their integration into caterpillar's genome.
title_full	Bracoviruses recruit host integrases for their integration into caterpillar's genome.
title_fullStr	Bracoviruses recruit host integrases for their integration into caterpillar's genome.
title_full_unstemmed	Bracoviruses recruit host integrases for their integration into caterpillar's genome.
title_sort	bracoviruses recruit host integrases for their integration into caterpillar's genome.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/80ac222e67bc44f1a608400f34952dc4
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Bracoviruses recruit host integrases for their integration into caterpillar's genome.

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