CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"

The Siberian tick-borne typhus (STT) is the most common tick-borne rickettsiosis (TBR) in Russia, registered in 17 administrative territories of the Southern Siberia and the Far East. The aim of this study was to describe clinical picture, pathological data and the results of laboratory diagnostics...

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Autores principales:	N. Rudakov, I. Samoylenko, S. Shtrek, S. Rudakova, L. Kumpan, Ya. Igolkina, V. Rar, E. Zhirakovskaya, S. Tkachev, N. Tikunova, T. Kostrykina, I. Blokhin, P. Lents
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	candidatus rickettsia tarasevichiae, rickettsia sibirica, etiological agents, rickettsioses, clinical manifestations, ixodid ticks Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/80b483822ff548a18ff76326598b4de8
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spelling	oai:doaj.org-article:80b483822ff548a18ff76326598b4de82021-11-22T07:09:56ZCLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"2220-76192313-739810.15789/2220-7619-CAL-1597https://doaj.org/article/80b483822ff548a18ff76326598b4de82019-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1597https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The Siberian tick-borne typhus (STT) is the most common tick-borne rickettsiosis (TBR) in Russia, registered in 17 administrative territories of the Southern Siberia and the Far East. The aim of this study was to describe clinical picture, pathological data and the results of laboratory diagnostics during fatal mixed infection caused by two rickettsia species most common in Russia - pathogenic Rickettsia sibirica and poorly examined Candidatus R. tarasevichiae, including identification of its etiological agents.A four-year-old girl in the Krasnoyarsk Territory in a hyperendemic focus of the Siberian tick-borne typhus after tick sucking revealed typical TBR symptoms (scab at the site of tick suction, fever, spotted rash, myalgia) and meningeal syndrome, which is not typical for the Siberian tick-borne typhus. The child died on the seventh day of the illness. Autopsy data (hepatosplenomegaly; cerebral edema, which was the immediate cause of death) and the results of histological examination (productive vasculitis of the brain, spinal cord and skin, polymorphic cell perivascular infiltrates in the liver and lungs, serous meningitis, myeloid hyperplasia of the spleen and lymph nodes, interstitial lymphoid infiltration in the myocardium) confirmed the clinical diagnosis of tick-borne rickettsiosis.The patient's blood and brain samples were tested for a wide range of tick-borne pathogens and enteric viruses that cause brain damage using PCR followed by sequencing of the positive samples.The DNA of Rickettsia sibirica and "Candidatus Rickettsia tarasevichiae" was found in both blood and brain samples. R. sibirica was identified by the nucleotide sequences of gene fragments gltA, OmpA and ompB, and "Candidatus R. tarasevichiae" - the gltA and ompB genes using nested PCR and sequencing. All amplified fragments were sequenced in both directions; the obtained sequences were deposited in the GenBank database under the inventory numbers: MK048467-MK048475. We have not identified other tick-borne pathogens or intestinal viruses in the patient samples able to result in meningeal syndrome.The area where the child was sucked by the tick belongs to the regions with a high incidence of STT. Several species of mites coexist in this area; of these, Haemaphisalis concinna, the carrier of R. sibirica, dominates the populations of Ixodes mites, while Ixodes persulcatus, the main reservoir of "Candidatus R. tarasevichiae", is less common.As a result of the conducted studies, for the first time in the Russian Federation, a verified case of a lethal infection associated with two species of rickettsiae - Rickettsia sibirica and "Candidatus R. tarasevichiae" was identified and described.N. RudakovI. SamoylenkoS. ShtrekS. RudakovaL. KumpanYa. IgolkinaV. RarE. ZhirakovskayaS. TkachevN. TikunovaT. KostrykinaI. BlokhinP. LentsSankt-Peterburg : NIIÈM imeni Pasteraarticlecandidatus rickettsia tarasevichiae, rickettsia sibirica, etiological agents, rickettsioses, clinical manifestations, ixodid ticksInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 0, Iss 0 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	candidatus rickettsia tarasevichiae, rickettsia sibirica, etiological agents, rickettsioses, clinical manifestations, ixodid ticks Infectious and parasitic diseases RC109-216
spellingShingle	candidatus rickettsia tarasevichiae, rickettsia sibirica, etiological agents, rickettsioses, clinical manifestations, ixodid ticks Infectious and parasitic diseases RC109-216 N. Rudakov I. Samoylenko S. Shtrek S. Rudakova L. Kumpan Ya. Igolkina V. Rar E. Zhirakovskaya S. Tkachev N. Tikunova T. Kostrykina I. Blokhin P. Lents CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"
description	The Siberian tick-borne typhus (STT) is the most common tick-borne rickettsiosis (TBR) in Russia, registered in 17 administrative territories of the Southern Siberia and the Far East. The aim of this study was to describe clinical picture, pathological data and the results of laboratory diagnostics during fatal mixed infection caused by two rickettsia species most common in Russia - pathogenic Rickettsia sibirica and poorly examined Candidatus R. tarasevichiae, including identification of its etiological agents.A four-year-old girl in the Krasnoyarsk Territory in a hyperendemic focus of the Siberian tick-borne typhus after tick sucking revealed typical TBR symptoms (scab at the site of tick suction, fever, spotted rash, myalgia) and meningeal syndrome, which is not typical for the Siberian tick-borne typhus. The child died on the seventh day of the illness. Autopsy data (hepatosplenomegaly; cerebral edema, which was the immediate cause of death) and the results of histological examination (productive vasculitis of the brain, spinal cord and skin, polymorphic cell perivascular infiltrates in the liver and lungs, serous meningitis, myeloid hyperplasia of the spleen and lymph nodes, interstitial lymphoid infiltration in the myocardium) confirmed the clinical diagnosis of tick-borne rickettsiosis.The patient's blood and brain samples were tested for a wide range of tick-borne pathogens and enteric viruses that cause brain damage using PCR followed by sequencing of the positive samples.The DNA of Rickettsia sibirica and "Candidatus Rickettsia tarasevichiae" was found in both blood and brain samples. R. sibirica was identified by the nucleotide sequences of gene fragments gltA, OmpA and ompB, and "Candidatus R. tarasevichiae" - the gltA and ompB genes using nested PCR and sequencing. All amplified fragments were sequenced in both directions; the obtained sequences were deposited in the GenBank database under the inventory numbers: MK048467-MK048475. We have not identified other tick-borne pathogens or intestinal viruses in the patient samples able to result in meningeal syndrome.The area where the child was sucked by the tick belongs to the regions with a high incidence of STT. Several species of mites coexist in this area; of these, Haemaphisalis concinna, the carrier of R. sibirica, dominates the populations of Ixodes mites, while Ixodes persulcatus, the main reservoir of "Candidatus R. tarasevichiae", is less common.As a result of the conducted studies, for the first time in the Russian Federation, a verified case of a lethal infection associated with two species of rickettsiae - Rickettsia sibirica and "Candidatus R. tarasevichiae" was identified and described.
format	article
author	N. Rudakov I. Samoylenko S. Shtrek S. Rudakova L. Kumpan Ya. Igolkina V. Rar E. Zhirakovskaya S. Tkachev N. Tikunova T. Kostrykina I. Blokhin P. Lents
author_facet	N. Rudakov I. Samoylenko S. Shtrek S. Rudakova L. Kumpan Ya. Igolkina V. Rar E. Zhirakovskaya S. Tkachev N. Tikunova T. Kostrykina I. Blokhin P. Lents
author_sort	N. Rudakov
title	CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"
title_short	CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"
title_full	CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"
title_fullStr	CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"
title_full_unstemmed	CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"
title_sort	clinical and laboratory characteristics of tick-borned rickettsiosis related to rickettsia sibirica and "candidatus rickettsia tarasevichiae"
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/80b483822ff548a18ff76326598b4de8
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_version_	1718417866187866112

CLINICAL AND LABORATORY CHARACTERISTICS OF TICK-BORNED RICKETTSIOSIS RELATED TO RICKETTSIA SIBIRICA AND "CANDIDATUS RICKETTSIA TARASEVICHIAE"

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