Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle

The genetic architecture of methane (CH<sub>4</sub>) production remains largely unknown. We aimed to estimate its heritability and to perform genome-wide association studies (GWAS) for the identification of candidate genes associated with two phenotypes: CH<sub>4</sub> in par...

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Autores principales:	Mateusz Sypniewski, Tomasz Strabel, Marcin Pszczola
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	methane emission dairy cows genome-wide association study Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/80b7c6c7b90c48f38b6193b763941024
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spelling	oai:doaj.org-article:80b7c6c7b90c48f38b6193b7639410242021-11-25T16:18:15ZGenetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle10.3390/ani111131752076-2615https://doaj.org/article/80b7c6c7b90c48f38b6193b7639410242021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3175https://doaj.org/toc/2076-2615The genetic architecture of methane (CH<sub>4</sub>) production remains largely unknown. We aimed to estimate its heritability and to perform genome-wide association studies (GWAS) for the identification of candidate genes associated with two phenotypes: CH<sub>4</sub> in parts per million/day (CH<sub>4</sub> ppm/d) and CH<sub>4</sub> in grams/day (CH<sub>4</sub> g/d). We studied 483 Polish Holstein-Friesian cows kept on two commercial farms in Poland. Measurements of CH<sub>4</sub> and carbon dioxide (CO<sub>2</sub>) concentrations exhaled by cows during milking were obtained using gas analyzers installed in the automated milking system on the farms. Genomic analyses were performed using a single-step BLUP approach. The percentage of genetic variance explained by SNPs was calculated for each SNP separately and then for the windows of neighbouring SNPs. The heritability of CH<sub>4</sub> ppm/d ranged from 0 to 0.14, with an average of 0.085. The heritability of CH<sub>4</sub> g/d ranged from 0.13 to 0.26, with an average of 0.22. The GWAS detected potential candidate SNPs on BTA 14 which explained ~0.9% of genetic variance for CH<sub>4</sub> ppm/d and ~1% of genetic variance for CH<sub>4</sub> g/d. All identified SNPs were located in the TRPS1 gene. We showed that methane traits are partially controlled by genes; however, the detected SNPs explained only a small part of genetic variation—implying that both CH<sub>4</sub> ppm/d and CH<sub>4</sub> g/d are highly polygenic traits.Mateusz SypniewskiTomasz StrabelMarcin PszczolaMDPI AGarticlemethane emissiondairy cowsgenome-wide association studyVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3175, p 3175 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	methane emission dairy cows genome-wide association study Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	methane emission dairy cows genome-wide association study Veterinary medicine SF600-1100 Zoology QL1-991 Mateusz Sypniewski Tomasz Strabel Marcin Pszczola Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle
description	The genetic architecture of methane (CH<sub>4</sub>) production remains largely unknown. We aimed to estimate its heritability and to perform genome-wide association studies (GWAS) for the identification of candidate genes associated with two phenotypes: CH<sub>4</sub> in parts per million/day (CH<sub>4</sub> ppm/d) and CH<sub>4</sub> in grams/day (CH<sub>4</sub> g/d). We studied 483 Polish Holstein-Friesian cows kept on two commercial farms in Poland. Measurements of CH<sub>4</sub> and carbon dioxide (CO<sub>2</sub>) concentrations exhaled by cows during milking were obtained using gas analyzers installed in the automated milking system on the farms. Genomic analyses were performed using a single-step BLUP approach. The percentage of genetic variance explained by SNPs was calculated for each SNP separately and then for the windows of neighbouring SNPs. The heritability of CH<sub>4</sub> ppm/d ranged from 0 to 0.14, with an average of 0.085. The heritability of CH<sub>4</sub> g/d ranged from 0.13 to 0.26, with an average of 0.22. The GWAS detected potential candidate SNPs on BTA 14 which explained ~0.9% of genetic variance for CH<sub>4</sub> ppm/d and ~1% of genetic variance for CH<sub>4</sub> g/d. All identified SNPs were located in the TRPS1 gene. We showed that methane traits are partially controlled by genes; however, the detected SNPs explained only a small part of genetic variation—implying that both CH<sub>4</sub> ppm/d and CH<sub>4</sub> g/d are highly polygenic traits.
format	article
author	Mateusz Sypniewski Tomasz Strabel Marcin Pszczola
author_facet	Mateusz Sypniewski Tomasz Strabel Marcin Pszczola
author_sort	Mateusz Sypniewski
title	Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle
title_short	Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle
title_full	Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle
title_fullStr	Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle
title_full_unstemmed	Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle
title_sort	genetic variability of methane production and concentration measured in the breath of polish holstein-friesian cattle
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/80b7c6c7b90c48f38b6193b763941024
work_keys_str_mv	AT mateuszsypniewski geneticvariabilityofmethaneproductionandconcentrationmeasuredinthebreathofpolishholsteinfriesiancattle AT tomaszstrabel geneticvariabilityofmethaneproductionandconcentrationmeasuredinthebreathofpolishholsteinfriesiancattle AT marcinpszczola geneticvariabilityofmethaneproductionandconcentrationmeasuredinthebreathofpolishholsteinfriesiancattle
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Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle

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